Bio113 Lab Schedule - Dr. Campbell at Davidson College

Your oral presentation will be divided into four sections: Introduction; Materials and Methods; Results; Discussion. Each person will present one section. The oral presentation must be pledged to indicate that each person made substantial contributions. You will not know which section you are presenting until 1 hour before lab, therefore you should learn all 4 parts.

For the final written lab report, each person must write his or her own (including figure legends), though you may confer about the content. The figures may be shared resources since they were produced as a team. Put your name as the first author and your labmates' names as coauthors.

Date	Topics		Lab Manual Word Files
August 24	Introduction	1) Safety Issues 2) Submit info to CATME for Team maker 3) baseline survey 4) How to Use a "Pipetman" 5) Synthetic Biology Introduction (PDF version) 6) TOSLS survey	Lab Manual Week #1 Practice Calculations
August 31	Information & Cells	Before Lab... 1) Answer 4 Questions 2 ) Create a Registry account (top right "login", apply for new account) 3) Radio show about Synthetic Biology (7 minutes) During Lab... 4) Basics of cloning DNA parts 5) Registry of Parts 6) Read papers pClone (J119137) paper one rClone (J119384) paper one actClone (J100204) paper one and paper two repClone (J100205) paper one and paper two 7) Home in on DNA control element to test 7) CATME first week	Lab Manual Week #2
September 7	Information & Cells	Before Lab... 1) Answer 4 Questions 2) What is golden gate assembly (GGA)? Overview and Detailed explanations During Lab... 3) Identify genetic control element DNA sequence 4) generate oligos from GGA Oligator 5) make a new part on Registry 6) name sequences and email for synthesis 7) one volunteer/group for next Wed. 4:30 pm 8) do calculations, steps 1-6 9) CATME peer review	Lab Manual Week #3
September 14	Information & Cells	Before Lab... 1) 4:30 pm day before lab, boil oligos (one per group) 2) Answer 4 Questions 3) View your receiving plasmid pClone rClone actClone repClone During Lab... 4) dilute oligos for ligation (step 2 lab manual) 5) do GGA ligation for oligos 6) do paper GGA activity + optional review session in lab 7) transform 3 tubes of E. coli ( N and P controls, plus X) 8) design next week's experiments (with controls) 9) one volunteer/group for next Wed. 4:30 pm 10) CATME peer review	Lab Manual Week #4
September 21	Information & Cells	Before Lab... 1) 4:30 pm day before lab, start cells growing (+/- treatment). 2) Answer 4 Questions During Lab... 3) start confirmation PCR, practice loading gel 4) test cells in Synergy machine 5) quantify Synergy data (use Excel) 6) update data in Registry 7) CATME peer review	Lab Manual Week #5
September 28	Information & Cells Oral Report Grading Rubric	Before Lab... 1) Answer 4 Questions 2) PCR animation During Lab... 2) run PCR products on gel (1 kb ladder) 3) prepare for oral presentation (iBOP Bingo) 4) CATME peer review	Lab Manual Week #6
October 5	Information & Cells Oral Report Grading Rubric	Before Lab... 1) Answer 4 Questions 2) prepare oral presentation, assigning email 1 hour before During Lab... 3) give oral presentation, read peer comments (iBOP Bingo) 4) choose the next iteration experiment (v2.0) Research Proposal Template 5) CATME peer review	Lab Manual Week #7
October 12	Information & Cells	Before Lab... 1) Evaluate experimental design v2.0 2) Answer 4 Questions During Lab... 3) write research proposal draft 4) CATME peer review	Lab Manual Week #8
October 19	Information & Cells	Before Lab... 1) review draft research proposal 2) DNA sequencing 3) Answer 4 Questions During Lab... 4) refine research proposal 5) generate oligos from GGA Oligator 6) make a new part on Registry 7) name sequences and email for synthesis 8) submit group research proposal for grading 9) one volunteer/group for next Wed. 4:30 pm 10) CATME peer review	Lab Manual Week #9
October 26	Information & Cells	Before Lab... 1) review GGA and transformation protocols 2) Answer 4 Questions During Lab... 3) dilute oligos for ligation (calculations last week) 4) do GGA ligation for oligos 5) transform E. coli (including + and - controls) 6) design experiments (with controls) 7) one volunteer/group for next Wed. 4:00 pm 8) CATME peer review	Lab Manual Week #10
November 2	Information & Cells	Before Lab... 1) review experimental design v2.0 2) Answer 4 Questions During Lab... 3) test cells in Synergy machine 4) quantify Synergy data (use Excel) 5) update data in Registry 6) CATME peer review	Lab Manual Week #11
November 9	Information & Cells	Before Lab... 1) read through mini-prep protocol 2) Answer 4 Questions During Lab... 3) miniprep plasmid DNA 4) nanodrop DNA to determine concentration 5) send DNA off to be sequenced 6) CATME peer review	Lab Manual Week #12
November 16	Information & Cells Written Report Grading Rubric	Before Lab... 1) review how cycle sequencing works (download ApE software - optional; on all college computers) 2) Answer 4 Questions During Lab... 3) analyze sequencing results (instructions here) 4) CLUSTAL omega web site download receiving plasmids, download your sequences 4) finalize all data from semester-long projects 5) prepare for final graded lab report (each person) 6) CATME peer review	Lab Manual Week #13
November 23		Thanksgiving Break	Week #14
November 30	Information & Cells	Before Lab... 1) write final lab report During Lab... 2) submit written lab report for grading (email Word file) 3) Fellowships for you 4) Lab evaluation 5) Course evaluation emailed to you	week #15 lab manual

Bio 113 Lab Schedule - Dr. C. Fall 2017

List of Protocols Used in Bio113

Practice Calculations

Dr. Campbell's Bio113 Page