Multicolor Immunofluorescence (computer assisted)

When you want to follow more than one protein at a time, you have to use antibodies with different colors on them, or use multiple cells. A computer program can help you merge the multiple images into a single image as shown in these figures. Here are some examples that let you see the power of labeling more than one protein at the same time.

These images were genrously provided by Stephen W. Paddock, Eric J. Hazen, and Peter J. DeVries, HHMI, University of Wisconsin, Madison, WI, USA.

This is an image that shows a Drosophila embryo at the cellular blastoderm stage triple-labeled for three segmentation proteins

hairy in red
Kruppel in green
giant in blue

This is an image of a Drosophila third instar wing imaginal disk double-labeled for:

apterous in red and achaete in geen.

Note what happens when there is an overlap of colocalized proteins (yellow).

Click here to see the original figures as they appeared in BioTechniques. 1997. 22: (1) 120 - 126.

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