E. coli amino acid sequence used shown in bold.
LOCUS ECAE000161 16170 bp DNA BCT 02-SEP-1997
DEFINITION Escherichia coli K-12 MG1655 section 51 of 400 of the complete
genome.
ACCESSION AE000161 U00096
NID g1786766
KEYWORDS .
SOURCE Escherichia coli.
ORGANISM Escherichia coli
Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
Escherichia.
REFERENCE 1 (bases 1 to 16170)
AUTHORS Blattner,F.R., Plunkett III,G., Bloch,C.A., Perna,N.T., Burland,V.,
Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
Mau,B. and Shao,Y.
TITLE The complete genome sequence of Escherichia coli K-12
JOURNAL Science 277 (5331), 1453-1474 (1997)
MEDLINE 97426617
REFERENCE 2 (bases 1 to 16170)
AUTHORS Blattner,F.R.
TITLE Direct Submission
JOURNAL Submitted (16-JAN-1997) Guy Plunkett III, Laboratory of Genetics,
University of Wisconsin, 445 Henry Mall, Madison, WI 53706, USA.
Email: ecoli@genetics.wisc.edu Phone: 608-262-2534 Fax:
608-263-7459
REFERENCE 3 (bases 1 to 16170)
AUTHORS Blattner,F.R.
TITLE Direct Submission
JOURNAL Submitted (02-SEP-1997) Guy Plunkett III, Laboratory of Genetics,
University of Wisconsin, 445 Henry Mall, Madison, WI 53706, USA.
Email: ecoli@genetics.wisc.edu Phone: 608-262-2534 Fax:
608-263-7459
COMMENT The E. coli K-12 sequence and its annotations have been updated.
All of the ambiguous residues in our original submission have been
resolved, and mis-assemblies in two repetitive regions have been
realigned. The annotations have been improved and updated as well.
With this release we begin designating a version number for the
annotated sequence, to assist in keeping track of corrections,
updates, and other changes. This is version M52 (SEPT. 02, 1997).
In addition, a revised notation has been instituted which assigns
each gene (protein- or RNA-encoding) a unique numeric identifier
beginning with a lowercase 'b' (in the '/label' field); this will
remain constant through further updates, gene identifications, etc.
This sequence was determined by the E. coli Genome Project at the
University of Wisconsin-Madison (Frederick R. Blattner, director).
Supported by NIH grants HG00301 and HG01428 (from the Human Genome
Project and NCHGR). The entire sequence was independently
determined from E. coli K-12 strain MG1655.
Predicted open reading frames were determined using GeneMark
software, kindly supplied by Mark Borodovsky, Georgia Institute of
Technology, Atlanta, GA, 30332.
e-mail: mark@amber.gatech.edu
Open reading frames that have been correlated with genetic loci are
being annotated with CG Site Nos., unique ID nos. for the genes in
the E. coli Genetic Stock Center (CGSC) database at Yale
University, kindly supplied by Mary Berlyn. A public version of the
database is accessible (http://cgsc.biology.yale.edu).
Annotation of the genome is an ongoing task whose goal is to make
the genome sequence more useful by correlating it with other data.
Comments to the authors are appreciated. Updated information will
be available at the E. coli Genome Project's World Wide Web site
(http://www.genetics.wisc.edu).
FEATURES Location/Qualifiers
source 1..16170
/organism="Escherichia coli"
/strain="K-12"
/sub_strain="MG1655"
/db_xref="taxon:562"
CDS 287..502
/note="o71; 85 pct identical amino acid sequence and equal
length to VLYS_BPP21 SW: P27360"
/codon_start=1
/label=b0554
/db_xref="PID:g1786767"
/transl_table=11
/translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
LVFGLLTYLTNLYFKIKEDKRKAARGE"
CDS 502..999
/EC_number="3.2.1.17"
/note="o165; 99 pct identical to LYCV_BPPA2 SW: P10439"
/codon_start=1
/label=b0555
/product="bacteriophage lambda lysozyme homolog"
/db_xref="PID:g1786768"
/transl_table=11
/translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
LWGQQ"
CDS 996..1457
/EC_number="3.4.-.-"
/note="o153; 96 pct identical to ENPP_LAMBD SW: P00726"
/codon_start=1
/label=b0556
/product="bacteriophage lambda endopeptidase homolog"
/db_xref="PID:g1786769"
/transl_table=11
/translation="MSRVTAIISALIICIIVSLSWAVNHYRDNAIAYKVQRDKNAREL
KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSV
REATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
CDS complement(1489..1782)
/note="f97; 96 pct identical amino acid sequence and equal
length to VBOR_LAMBD SW: P26814"
/codon_start=1
/label=b0557
/product="bacteriophage lambda Bor protein homolog"
/db_xref="PID:g1786770"
/transl_table=11
/translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
CDS complement(2073..2525)
/note="f150; phage stats; 24 pct identical (6 gaps) to 125
residues of approx. 2136 aa protein YCF2_SPIOL SW: P08973"
/codon_start=1
/label=b0558
/db_xref="PID:g1786771"
/transl_table=11
/translation="MQTTRPRITWKVLPMAQVAIFKEIFDQVRKDLNCELFYSELKRH
NVSHYIYYLATDNIHIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITF
QQYRENLAKAGVFRWITNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
CDS 2769..2975
/note="o68; 28 pct identical (3 gaps) to 57 residues of
approx. 1488 aa protein CFTR_XENLA SW: P26363"
/codon_start=1
/label=b0559
/db_xref="PID:g1786772"
/transl_table=11
/translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
AFRAIEAALVKHDNNMKDYSLVVD"
gene 3723..4268
/gene="nohB"
CDS 3723..4268
/gene="nohB"
/note="o181; 100 pct identical to fragment NOHB_ECOLI SW:
P31062 (147 aa); 98 pct identical to TERS_LAMBD SW:
P03707"
/codon_start=1
/label=b0560
/product="DNA packaging protein"
/db_xref="PID:g1786773"
/transl_table=11
/translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
AVIKWYAERDAEIENEKLRREVEELLQASETDLQPGTIEYERHRLTRAQADAQELKNA
RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
KAAALDELIPGLLSEYIEQSG"
CDS 4243..4986
/note="o247; residues 1-103 are 100 pct identical to aa
1-103 from TERL_LAMBD SW: P03708 (641 aa); residues
166-233 are 66 pct identical to aa 30-97 from YCDD_SALTY
SW: P40784 (106 aa)"
/codon_start=1
/label=b0561
/db_xref="PID:g1786774"
/transl_table=11
/translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
IPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEFQ
KWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEETSLLE
AWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
CDS complement(5041..5472)
/note="f143; This 143 aa ORF is 30 pct identical (7 gaps)
to 96 residues of an approx. 568 aa protein NPRM_BACME SW:
Q00891"
/codon_start=1
/label=b0562
/db_xref="PID:g1786775"
/transl_table=11
/translation="MDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSIS
MSYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQK
GIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
CDS 5764..5949
/note="o61; 100 pct identical to hypothetical E. coli
protein from Genbank Accession U82598; residues 32-61 are
80 pct identical to aa 137-166 from tail fiber assembly
protein, Genbank Accession D90798"
/codon_start=1
/label=b0563
/db_xref="PID:g2367110"
/transl_table=11
/translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
AVDLEIATEEERSLLEA"
gene 6570..7319
/gene="appY"
CDS 6570..7319
/gene="appY"
/note="o249; 100 pct identical to GB: ECM5_1 ACCESSION:
Y00138; 100 pct identical to 219 aa of APPY_ECOLI SW:
P05052 (243 aa)"
/codon_start=1
/label=b0564
/product="M5 polypeptide"
/db_xref="PID:g1786776"
/transl_table=11
/translation="MDYVCSVVFICQSFDLIINRRVISFKKNSLFIVSDKIRRELPVC
PSKLRIVDIDKKTCLSFFIDVNNELPGKFTLDKNGYIAEEEPPLSLVFSLFEGIKIAD
SHSLWLKERLCISLLAMFKKRESVNSFILTNINTFTCKITGIISFNIERQWHLKDIAE
LIYTSESLIKKRLRDEGTSFTEILRDTRMRYAKKLITSNSYSINVVAQKCGYNSTSYF
ICAFKDYYGVTPSHYFEKIIGVTDGINKTID"
gene complement(7569..8522)
/gene="ompT"
CDS complement(7569..8522)
/gene="ompT"
/EC_number="3.4.21.87"
/note="f317; 100 pct identical to OMPT_ECOLI SW: P09169"
/codon_start=1
/label=b0565
/product="protease VII precursor"
/db_xref="PID:g1786777"
/transl_table=11
/translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
gene complement(9036..9797)
/gene="envY"
CDS complement(9036..9797)
/gene="envY"
/note="f253; This 253 aa ORF is 98 pct identical (2 gaps)
to ENVY_ECOLI SW: P10805"
/codon_start=1
/label=b0566
/db_xref="PID:g1786778"
/transl_table=11
/translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
NHSKQQPSRPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
gene complement(9980..10870)
/gene="ybcH"
CDS complement(9980..10870)
/gene="ybcH"
/note="f296; 99 pct identical to 130 aa of YBCH_ECOLI SW:
P37325 (145 aa) but has 167 alternate C-terminal residues"
/codon_start=1
/label=b0567
/db_xref="PID:g1786779"
/transl_table=11
/translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
TAEQRERYLQASADCQSPAPASGVVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
KDTLYFSLRYLPVAHGILEY"
gene complement(10871..13843)
/gene="nfrA"
CDS complement(10871..13843)
/gene="nfrA"
/note="f990; 100 pct identical to NFRA_ECOLI SW: P31600"
/codon_start=1
/label=b0568
/product="bacteriophage N4 adsorption protein A"
/db_xref="PID:g1786780"
/transl_table=11
/translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEPAHKGLPDDPAL
IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFIAVEQQLPLNGQNGASDTMLRA
SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
NQRNGERNNAFLTIGVHW"
gene complement(13830..16067)
/gene="nfrB"
CDS complement(13830..16067)
/gene="nfrB"
/note="f745; 100 pct identical to NFRB_ECOLI SW: P31599"
/codon_start=1
/label=b0569
/product="bacteriophage N4 adsorption protein B precursor"
/db_xref="PID:g1786781"
/transl_table=11
/translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
ENEGE"
BASE COUNT 4164 a 3582 c 3915 g 4509 t
ORIGIN
1 ttggttattt gttgagattt gcttatgtat ttgtagtggt gttttcaata ctcggtagca
61 ttctctcaaa tatcatttag tggtttacgt acgtaaaaaa ttggttatgc tgttaagagt
121 ggttacttcg tcacacagct taaacccgcc gtcgagcggg tttttccatt ttttgagtct
181 cgatattagc tgataaccca atacctgagt tattcactga ctccgagtct gttacgtttc
241 gtagtattcc ctcaatttac acccgctttg tctgcgaggt ggggttatga aatccatgga
301 taagttaaca acgggtgtcg cctatggcac ctcagcaggt agtgccgggt actggttttt
361 acagctgcta gataaagtca ctccctcaca gtgggcagca ataggtgtgc tgggtagcct
421 ggtatttggc ctgctgacgt acctgacaaa cctttatttc aagattaaag aagataagcg
481 caaggctgcg agaggtgaat aatgcctcca tcattacgaa aagccgttgc tgctgctatt
541 ggtggcggag caattgctat agcatcagtg ttaatcactg gcccaagtgg taacgatggt
601 ctggaaggtg tcagctacat accatacaaa gatattgttg gtgtatggac tgtatgtcac
661 ggacacaccg gaaaagacat catgctcggt aaaacgtata ccaaagcaga atgcaaagca
721 ctcttgaata aagaccttgc cactgtcgcc agacaaatta acccgtatat caaagtcgat
781 ataccggaaa caacgcgcgg cgctctttac tcattcgttt acaacgtggg tgctggcaat
841 tttagaacat cgacgcttct tcgcaaaata aaccagggcg atatcaaagg cgcatgtgat
901 cagctgcgtc gctggacata cgctggcggt aagcaatgga aaggcctgat gactcgtcgt
961 gagattgagc gtgaagtctg tttgtggggg caacagtgag cagagtaacc gcgattatat
1021 ccgctctgat tatctgcatc atcgtcagcc tgtcatgggc ggtcaatcat taccgtgata
1081 acgcaatcgc ctacaaagtc cagcgcgaca aaaatgccag agaactgaag ctagcgaacg
1141 cggcaattac tgacatgcag atgcgtcagc gtgatgttgc tgcgctcgat gcaaaataca
1201 cgaaggagtt agctgatgcg aaagctgaaa atgatgctct gcgtgatgat gttgccgctg
1261 gtcgtcgtcg gttgcacatc aaagcagtct gtcagtcagt gcgtgaagcc accacggcct
1321 ccggcgtgga taatgcagcc tccccccgac tggcagacac cgctgaacgg gattatttca
1381 ccctcagaga gaggctgatc actatgcaaa aacaactgga aggaacccag aagtatatta
1441 atgagcagtg cagatagagc tgaccatatc gatgggcaac tcatgcaatt attttgagca
1501 atacacacgc gcttccagcg gagtataaat gcctaaagta ataaaaccga gcaatccatt
1561 tacgaatgtt tgctgggttt ctgttttaac aacattttct gcgccgccac aaattttagc
1621 tgcatcgaca gttttcttct gcccaattcc agaaacgaag aaatgatggg tgatggtttc
1681 ctttggtgct actgctgtct gtttgttttg aacagtaaat gtctgttgag cacatcctgt
1741 aataagcagg gccagcgcag tagcgagtag catttttttc atggtgttat tcccgatgct
1801 ttttgaagtt cgcagaatcg tatgtgtaga aaattaaaca aaccctaaac aatgagttga
1861 aatttcatat tgttaatatt tattaatgta tgccaggtgc gatgaatcgt cattgtattc
1921 ccggattaac tatgtccaca gccctgacgg ggaacttctc tgcgggagtg tccgggaata
1981 attaaaaacg atgcacacag ggtttagcgc gtacatgtat tgtattatgc caacaccccg
2041 gtgctgacac ggaagaaacc ggacgttatg atttagcgtg gaaagatttg tgtagtgttc
2101 tgaatgctct cagtaaatag taatgaatta tcaaaggtat agtaatatct tttatgttcg
2161 tggatatttg taatccatcg gaaaactcct gctttagcaa gattttccct gtattgctga
2221 aatgtgattt ctcttgattt caacctatca taggacgttt ctataagatg cgtatttctt
2281 gagaatttaa catttacaac ctttttaagt ccttttatta acacggtgtt atcgttttct
2341 aacacaatgt gaatattatc tgtggctaga tagtaaatat aatgtgagac attgtgacgt
2401 tttagttcag aataaaacaa ttcacagttt aaatcttttc gcacttgatc gaatatttct
2461 ttaaaaatgg caacctgagc cattggtaaa accttccatg tgatacgagg gcgcgtagtt
2521 tgcattatcg tttttatcgc ttcaatctgg tctgacctct ttgtgttttg ttgatgattt
2581 atgtcaaata ttaggaatgt tttcaattaa tagtattggt tgcgtaacaa agtgcggtcc
2641 tgctggcatt ctggagggaa atacaaccga cagatgtatg taaggccaac gtgctcaaac
2701 cttcatacag aaagatttga agtaatattt taaccgctag atgaagagca agcgcatgga
2761 gcgacaaaat gaataaagaa caatctgctg atgatccctc cgtggatctg attcgtgtaa
2821 aaaatatgct taatagcacc atttctatga gttaccctga tgttgtaatt gcatgtatag
2881 aacataaggt gtctctggaa gcattcaggg caattgaggc agcgttggtg aagcacgata
2941 ataatatgaa ggattattcc ctggtggttg actgatcacc ataactgcta atcattcaaa
3001 ctacttaacc tgtgacagag ccaacacgca gtctgtcact gtcaggaaag tggtaaaact
3061 gcaactcaat tactgcaatg ccctcgtaat taagtgaatt tacaatatcg tcctgttcgg
3121 agggaagaac gcgggatgtt cattcttcat cacttttaat tgatgtatat gctctctttt
3181 ctgacgttag cctccgacgg caggcttcaa tgacccaggc tgagaaattc ccggaccctt
3241 tttgctcaag agcgatgtta atttgttcaa tcatttggtt aggaaagcgg atgttgcggg
3301 ttgttgttct gcgggttctg ttcttagttg acatgaggtt gccccgtatt cagtgtcgct
3361 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
3421 ctgcgtcata attgattatt tgacgtggtt tgatggcgta gatgcacgtt gtgacatgta
3481 gatgataatt attatcattt tgtgggtcct ttccggcgat ccgacaggtt acggggcggc
3541 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggtgttt ccgttcttct
3601 tcgtcgtaac ttaatgtatt tatttaaaat accccctgaa aagaaaggaa acgacaggtg
3661 ctgaaagcga gctttttggc ctctgtcgtt tcctttctct gtttttgtcc gtggaatgtg
3721 caatggaagt caacaaaaag cagctggctg acattttcgg tgcgagtatc cgtaccattc
3781 agaactggca ggaacaggga atgcccgttc tgcgaggcgg tggcaagggt aatgaggtgc
3841 tttatgactc tgccgccgtc ataaaatggt atgccgaaag ggatgctgaa attgagaacg
3901 aaaagctgcg ccgggaggtt gaagaactgc tgcaggccag cgagacagat ctccagccag
3961 ggactattga gtacgaacgc catcgactta cgcgtgcgca ggccgatgca caggagctga
4021 aaaatgccag agactccgct gaagtggtgg aaaccgcatt ctgtactttc gtgctgtcgc
4081 ggatcgcagg tgaaattgcc agtattctcg acgggatccc cctgtcggtg cagcggcgtt
4141 ttccggaact ggaaaaccga catgttgatt tcctgaaacg ggatatcatc aaagccatga
4201 acaaagcagc cgcgctggat gaactgatac cggggttgct gagtgaatat atcgaacagt
4261 caggttaaca ggctgcggca ttttgtccgc gccgggcttc gctcactgtt caggccggag
4321 ccacagaccg ccgttgaatg ggcggatgct aattactatc tcccgaaaga atccgcatac
4381 caggaagggc gctgggaaac actgcccttt cagcgggcca tcatgaatgc gatgggcagc
4441 gactacatcc gtgaggtgaa tgtggtgaag tctgcccgtg tcggttattc caaaatgctg
4501 ctgggtgttt atgcctactt tatagagcat aagcagcgca acacccttat tccagctggc
4561 ttcgtggctg ttttcaacag tgatgagtca tcgtggcatc tcgttgaaga tcatcggggt
4621 aaaacggttt atgacgtagc gtcaggggac gcgttattta tttctgaact cggtccgtta
4681 ccggaaaatg ttacctggtt atcgccggaa ggggagtttc agaagtggaa cggtacagcc
4741 tgggtgaaag atgcagaagc agaaaaactg ttccggattc gggaggcgga agaaacaaaa
4801 aacagcctga tgcaggtagc cagtgagcat attgcgccac ttcaggatgc tgtagatctg
4861 gaaatcgcaa cggaggaaga aacctcattg ctggaagcct ggaaaaaata tcgggtgttg
4921 ctgaaccgtg ttgatacatc aactgcacct gatattgagt ggcctacgaa ccctgtcagg
4981 gagtaatcat tgggattatg ccgcagcacg tcttaagcaa gaacatgctg cggttggatg
5041 ctattttttt cctgaagcgg aaaacattac tacagtacct tgaaccttgg ttttaacatt
5101 ctcgaaatgc tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa
5161 gatgcctttc tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc
5221 atcgccaaga attgtggctc cgtatagagt tccatcgtca gttaaggcct gcgccgcatt
5281 gcgtattaca cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaagacat
5341 ggaaatggaa tcaaattgac catgtaacgc cgcgggataa ggttcaaaaa catcatggct
5401 aattttatgt ttaatttttg attccccagc ccttgtagat gccgcgttca ggctagcttc
5461 gttcaaatcc attaaagata tcagactact ctcaggtacg tgagtaaggt aaaacccagt
5521 tccaacacca atatccagat ggttgttacc taaatgttcc agaaagtgtg gaagaaggtg
5581 ttcctttgta ggacatcccc atgcaagccg atttgatact cccaaaaccc accagtcata
5641 aagctttagg gtaagtggtg tgtaaattct agccccatca tctgtgtttt tttattaatt
5701 tcaccatgtt atagttttat ttgtgaatta aatcaattat ggcaatgaat tacaaggggt
5761 taaatgctgc cgcagcatag cgatattgaa atagcctggt atgcttcaat acagcaggag
5821 ccgaatggct ggaagaccgt caccacacag ttctacatcc aggaattcag tgagtatatt
5881 gcgccactgc aggatgctgt agatctggaa atcgcaacgg aggaagaaag atcgttgctg
5941 gaagcctgaa aaaagtatcg ggtgctgcta aaccgtgtgg acacttccgt agcaccagat
6001 atcgagtggc ttattcaacc ataataaaca gtatgtatat cataggttat taattgtgag
6061 ttttttcggt gtgttatttg tttgtttgat gttatgcttt tgcgccccaa aaggttgttt
6121 agatgtattt tatcaattga ttttcaatat cgtttaataa agaaaaatta agcaagctgg
6181 atgttggttt tttgttaatt gaatggttct aataatgttt ttttactgtt gttgaatgtg
6241 acttgataag aaatgcaagt aaaaatgata ctctttttat tttaaattca aacggttgac
6301 atatatatag caagaggttt caggtgcgtt gtagtgagtt tatgttaata aaaagcatag
6361 taagcgttga aaaatgtaac tttgaaataa gttagaataa aaaacaacat acatataata
6421 atttaatctt aaatgaaatt tattaaaatt tgcaaactat aattttgtgt ataaaaatat
6481 aaatgcacat catcctgatt atgattgtgt atttaattgg ttgttatttg actactatca
6541 acttgtttta attttatgat aggtgcaaga tggattatgt ttgctccgta gttttcatct
6601 gtcaatcatt tgatttaatt ataaacagga gagttatctc gttcaaaaaa aattcattgt
6661 ttattgtaag cgacaaaatt agaagggagt taccagtatg cccctctaaa ctaagaattg
6721 ttgatataga taagaaaaca tgtttatcct tttttatcga cgtgaataat gagctgcctg
6781 gcaaatttac tcttgataag aatggctata ttgctgaaga ggaacctcca ttatcgcttg
6841 ttttttctct gtttgaaggg attaaaatag cagactcaca ctccctttgg ttaaaagaaa
6901 gactatgtat atccttactt gccatgttca aaaaacgcga aagtgtaaat tcatttatac
6961 taacaaatat aaatacattt acctgtaaaa ttactggaat aatcagtttt aatattgagc
7021 ggcaatggca tttaaaagat attgcggaat tgatttatac gagtgaaagt ttaataaaaa
7081 aaagattaag ggatgaagga acgtcattta ctgaaatatt gagagatact aggatgaggt
7141 atgcaaaaaa actcataact tcaaactctt attctatcaa tgtcgtagcc cagaaatgtg
7201 gctataacag tacttcatat ttcatatgtg catttaaaga ttattatggt gtcacgccat
7261 ctcattattt tgagaaaata atcggcgtca cagatggaat aaacaaaaca attgactgat
7321 aatgtttatt acaagttgtc tacatgttaa ttataatatt atacagcgtt ttttttgatg
7381 tgatattctg gaaccattaa tttgtaattg ggttgctgtc gcctatttta tacatactat
7441 aattgatggt tttctatgtg atttagttaa taaccttctg ggtttatttt aagggttaat
7501 tgttacattg aaatggctag ttattccccg gggcgatttt cacctcgggg aaattttagt
7561 tggcgttctt aaaatgtgta cttaagacca gcagtagtga tgaagttata gttttctata
7621 cctgctccat ttttgctgta gtctgaagtg ttattattgt gatcataaag tgaagtatta
7681 ccttttttat tcgtaacccg attccatgcg ccttcaacat aaacttttgc gttaggtgtg
7741 acgtaataac ctgcattgac tgcaacagaa tagtaatttt ggtctttgac cttactgcga
7801 taagtgattc tttttcccgg gtcatagtgt tcatcgttat cagatgattc cacccagccg
7861 ctgtatttaa atgtgccacc gagttcaaaa tcttcataac gataacttcc agtcaagcca
7921 atgtagggca ttttaaaacg ttgtttgtag ccgattgctc tttctccatt cgggaaggag
7981 ccgatatcat ctctgaatcc ctcctcagaa ctgtagatat aggaaccacc tctggctgta
8041 aagctataac ggctttcctg atatccggcc atgagtccca ggcggtaatt gggttcgttg
8101 aggagccagc ctttgatatt cagatcaaat tcgttggcat aattgagttg tgtatcaggg
8161 tgtctacttt catccgtcca ggttccgggg ttactggaat ccatccagtc ctgatcgacc
8221 atattgccac ctcggctgcc gagagttgtc cagccagcag ccccgataga tatctggggc
8281 atcaaatccc aattaattgc acctttaata attgcagcgt tattgaattt ccagtcgagt
8341 tgactgactt ttcggcctcc ttcttcggct agataaacac gctcttttgt ttttccgctc
8401 agagttccaa gactaatgtc cgcatttatg ttgtcaggag taaacgataa agtctcggta
8461 gaagcaaaag agctgatcgc aataggggtt gtcaggacta ttcccagaag tttcgcccgc
8521 ataaaagttc tccattcaat cgttttaatg attgaatatg tattttttat atctaactta
8581 atgagtcaat tacatattgc tccactgttt atattttgtt tagtattgaa tgattatcac
8641 aatgcgctat ctgtttttgg tttaattatc tgttattgtt tcatatttcg gttttactgt
8701 gtggtttttt tatgcttttg tggtgctttt atctatttaa gtgccatgcc tttagaggca
8761 tataagcgaa aatagcatga ggtttatcct caattactat gttttttagt acaaaaaaga
8821 gggacaaaac tgagacacat aaggcctcgc aatggcttgc aaggctttac atgttttgag
8881 gtagtgggac gtgtgagcgc agagatggcg cggtaagttg ttgacttaaa atgtcgttct
8941 aggaacttct aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcctc
9001 atttatgccc gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt
9061 ttggcgagat aattcaacgg tgtcaggccg taaaacgcct taaaaacaga gataaagtac
9121 gacgtgctgc tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat
9181 aacatctgta cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt
9241 tcgtttttta atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt
9301 cgcagattcc agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg
9361 ctgctgcgta agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac
9421 aatacggtaa aaagcaatgc gcgcgtcaac tccgcttcgc agggtctgct gggttgctgc
9481 ttgctgtggt tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc
9541 ggggttgcca gccgcgacca gggctttaca caggttaagt tcttatttaa aaacaacaga
9601 taatcgttga tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt
9661 tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta
9721 gcatggttat tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca
9781 ctgctgctca attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat
9841 gcttcctgca tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcacgctt
9901 tttagcaatt aatcttgatg gaattctgat gagagcgaaa gaggtaagcc aggtcgtacc
9961 cgacttacct ggaggagatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca
10021 gagagaaata gagagtgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt
10081 ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aaaagttcat
10141 aaacaacgcc gctggcaggg gcgggacttt ggcaatcggc gctggcctgt aaataacgtt
10201 cacgctgttc agcggtcagt ttatccacgc cgctgccatc ctgtacccag acattaacgc
10261 cggttgcttt aacgtgttcc agcagttggc gatagccatc gggcgacatg tttccggcga
10321 aaaaactact gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt
10381 ttaaccatgt tagcaaaggc tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt
10441 ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt
10501 gctggagatc ggcagccagc aggcgattaa gatagctttc cagcgctgcg gacgactgtt
10561 tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac aataagcttc agaccagcct
10621 gttgcgcagc tgcggcccgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat
10681 cgccgtaacg ggtccattgc aaaacaaggg tatcgaagcc ttgcaaacgt aactgactca
10741 tcagcccctg ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa
10801 tacctttcat cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga
10861 acttacgcat ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac
10921 gttgattaat cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc
10981 aggcgtcgta gtgcgtctcg ccggtccaga tattccagcg gaccccgact ccgccaagct
11041 gcgcgccctg agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa
11101 tagtctgtcc gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct
11161 gaatatcctg gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg
11221 aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc
11281 gcagcatggt atcggatgcg ccattttggc cgttcagcgg caactgctgt tcgacggcga
11341 tgaaaaagat ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt
11401 tcaccggcat catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga
11461 gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt
11521 agctacgata gcttttccct ggcgctgcgc cgccgacatt attgttagcg gtactcattg
11581 cgccggaacg caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct
11641 catgcaaacg gcggaaattg aagcgttgtt gattttgttc tggggtcagt ggggttatca
11701 gcgcctgatt atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt
11761 catccagacg ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc
11821 ctttatgcgc cggttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg
11881 cgtaaccaag cgctgcctgg gtgttgctat tattcggttc cagttccagc gcggcgcgca
11941 aatcactcac cgcggccggg acattatgac gttggcgata aattgtcgcc cgcgcaacgt
12001 aagcgttggc agaaggcgca atattgattg agcgcgtgag atcgttcagt gcgagttccg
12061 gctgaccagg aatgtaacgt tgcgcatgca gccaccagta gagggcattg cttcccagtc
12121 cacgtttttc tgcctgttgt agccagcgat cgcgagccgc accatttcct gccgcctggg
12181 cggtattggc agcagcaagc agatcctcat tgctcatgtc gtgaagactg attttctgcc
12241 aggccgccag tgcggtggcg tagtcctcaa cctgatacgc ctgataggct accgcacgat
12301 gttgccaggc gctcggttgt cgttgttcgg cctgaagcca tgcatacaac gccacaccgg
12361 gtagcgtgtc ccgataacac tttgccagac ggttccaggc ggcggcatcg taggaaggcg
12421 acatatcgcc cagcaagcga actattgccg ggcaattatc tgcaataccc ggcaactgac
12481 tttgccactg acgttgctcc gccagcggta agggtttcga taaaatcgcc accttcgccg
12541 gcgttgccag gtaaggatga ctttccagca gagacgccag tcgcgccatt aaagtctggc
12601 tgacacgcgc atcgccctgg aaaggatagc gttgcagcaa taaatcggca gcttcgcgtg
12661 actgctcgtt ctgcatcagt tgccaggtta gttgatccag gcgggtaaga tttgccggtt
12721 cttgctgata cagcaatcgt gccagacgca gagcttcagc cttgttacgg gtcgccacgc
12781 tgacagcata acgctcctca agcatttcat tggcggggag ggtggcgagc agtttttgcg
12841 ctgcgtcgta ctgaccttct tttaacagca ccggtagcgt cgcgccaaca acatactggc
12901 ggttgtcggc aaactgtacc gtataattcg ccaacgcctg aacggggtta gcgctgtatt
12961 tagataacag atagagccaa cttttctctt gtgcgtccgt ggtaaatagt ggcttatttt
13021 caatgagata atgctggagg cgtgcttttt cgccacgata agccagcgcg gtcgcgtaag
13081 taatatatga ctgaggatcg gtgaagatcc cctgtgattg cagtgccagg atccgatcgt
13141 ccagctgccc ggcaagaagc acgtcaaacc actgacggcg ttctgccgcg cttaatgtgt
13201 tctgctggcg tgcttcattg tatagcgtat ctgcctggga ccattgtttc aggtagattg
13261 cccgttgcag cagatcggtt cgcagcgttt ttccttccgg cgatgcagca aacgtcgcat
13321 cgttcagttg cgctctggcg acaggtaact gtgccagccg cagggcattc tgcccgactt
13381 cactgcgaca acgcagggtc ggcgcagcat cgcacgcttt ttgctgggca agcagttctt
13441 caactgtcgt aacgcttttc acttcaaccg gaatagccgc cagactgcgc tcaagtcggg
13501 catctcctgg gtgacgtttc agttgatcct caagcaacag ccgcgcccgg tcatcatgac
13561 caaaatggcg ataggcttcc gcaaggtata aagtcagcgg aatattatcc ggcacctgct
13621 ggtgtatata ttcaaattcg cggatggcgg ttgcttcgtc gttatttttc tgtgccttca
13681 gcgctttatc gagacgggga taaataacaa aatggcgata atcgctcagc cccagctctt
13741 ctgcgctggt gccgatattg tctgcgagtg cgctggtact caataaagac gtcagcagta
13801 aaccagacca tccgatgacg cgattaaggt tattctcctt cattttcgga ctccagttgc
13861 gcaacctgtt ctgtgtttaa acctgctttg agtaatagtg attgcatcga aacttgtaat
13921 tcgcgttgaa ttgtcaggac gcgatccaac gtttcctggc tgataacgcc ttcggtgacc
13981 aaaaacttgc cgagcggcag agaactgcgt tcatggcgca ataacaacac gttaattgct
14041 gaacgattaa tatgaccgag cgtggtcagt atttcggcga acaggaactg atgcggcaca
14101 tattgccgcc agatttcacc ggcctgctgt tccgtgagcc actgatgctg aaccgcattg
14161 tacaacattg cccgcggatc gtgaccgcgt cggcgtgcat accagtgacg taaccctgtg
14221 acaatttgtc cccgcagaac aatgacgtaa cgcactttgc gtccgacttt acgcgtcagg
14281 gccgccagcg aaaccgggtc aataccatct tcactgccga caattaactc gtcattttcc
14341 agacgcagcg gcagtaccgc ataatgcagc gccacggagg ccggcatttc ggcaatcagc
14401 gaggaaggga tctgccaggc atcgatggat tcccacgcca cgccgttttg ctctgccagc
14461 gcctgtgcca gctgctcggc gctaatcagc ccctgcatca gcattgaacc gcccaggcgt
14521 agaccttcga cgcgattacg cagtgctgta tcgagttgtt cttcagtgat gacctgattt
14581 tccagcagaa tttgacctaa cgggcgcaac gagcgggtat cgccagtcac gctggggaag
14641 tcatgcgttg ttttatccca cgccacgcga cgtggatcgc cgtgttgaag tacctgtttt
14701 agcgcgcgcc agttggccat gaagttaatc aggttgcccc agaaaagacg caggacggaa
14761 agcagcccct gcgtcaggcc gtagtagcca gtaacgaaaa tcacccgctg cacgatgcgg
14821 ttaaccatca aaccaaagtt tagccacagc agggtcatta accatgcgct gccgctgaaa
14881 atagaaagga aatgccaggc atcgggccac aaactttcat acgccagcaa cagcaaaagc
14941 tggatcatca ccagcatcgc gaggaagctg acaaagttac tgattgcccc tttgcggtcg
15001 cgccagagaa agtagttcag cgtcaggctg gaggtccatt tatgggtttt aaagccttgg
15061 aaaacaatgc cgatgatcca gcgggatttt tgtcgaaccg cagtcgaaaa ggtatcgggg
15121 aaatattcgc gcacgcagat catgtttgat gtccgcgcgt gctgtaaaaa tttacgctgc
15181 tcgcgttctt tggcttcgtc caccaccgga aaacggacaa aaatttccgt catacctttt
15241 tctttcaggc ggaagccaat gtcgtaatct tcagtaagac tctgcacgtc gaaagcaata
15301 ccgtcaccgt cagctaacag tgcggtcacg gcgcggcggc tgaaacaggt gccgacgcct
15361 gcgctgggca cttgtccggc gagggcttca cgcaccggaa catctttgcc atgcagctct
15421 gaaaactcat caatgtaagt catgctggtg aagtgcgtcc attcgcgttc gaacggatac
15481 accgggatct gaatcagatc tttacgctcg accagatagt tgaacagacg caattccatc
15541 ggtgaaatca catcttcggc gtcatgcaga ataaaaccag caaaagcgaa attggcgcta
15601 cgctcaaatt gggtgatggc gtccagcacg ttgttcagac agtcggcttt gctggtgggg
15661 ccaggacgcg cgcagactac cttatgcaca ttcgggaagc gagcgcacac ttcgtcaaca
15721 tcacgctgag tatcggggtc gttggggtag gtgccaacaa agatatgata gttttcgtag
15781 tcgagcgtgg tcgccgccag ctcggccata ttgccgatga cgcccgtttc attccacgcc
15841 ggaaccataa tcgctaacgg tttttcatct ggtttataca gttcgcggta actcattcgc
15901 gggtagcggc gataaacact caacttgcgt ttaatgcggc gtacccagta gacgacatca
15961 ataaaaaaat cgtccagccc gctgatgaac atgatgaccg ctaacgttat cgcgattact
16021 tttaagccgt agagccaggt agcaaaaaca tcaagaagcc agtccacaca aaaaccttac
16081 attaacgctg gttatgttta gggtggcgta tattaaggtt ttttatgaat tgtgacagct
16141 ttttaccatt aataggtatg actattgcgg
//