QUESTION 7 B
1) Protein Name: TMV resistance protein N
ORGANISM Nicotiana glutinosa
Eukaryotae; mitochondrial eukaryotes; Viridiplantae; Charophyta/Embryophyta group; Embryophyta; vascular plants; seed plants; Magnoliophyta; Magnoliopsida; Solananae; Solanales; Solanaceae; Nicotiana.
2) yes, there is another protein from another species that has a high degree of amino acid sequence similarity. By running a blast search with the target sequence IEESQFAIVVFSENY, one protein with a similar sequence was found. The total sequence is not the same, but there is a high degree of sequence similarity between the two. Below are the amino acid sequences of both proteins.
Amino acid sequence from TMV resistance protein N:
" ORIGIN 1 massssssrw sydvflsfrg edtrktftsh lyevlndkgi ktfqddkrle ygatipgelc 61 kaieesqfai vvfsenyats rwclnelvki mecktrfkqt vipifydvdp shvrnqkesf 121 akafeehetk ykddvegiqr wrialneaan lkgscdnrdk tdadcirqiv dqissklcki 181 slsylqnivg idthlekies lleigingvr imgiwgmggv gkttiaraif dtllgrmdss 241 yqfdgacflk dikenkrgmh slqnallsel lrekanynne edgkhqmasr lrskkvlivl 301 ddidnkdhyl eylagdldwf gngsriiitt rdkhlieknd iiyevtalpd hesiqlfkqh 361 afgkevpnen feklslevvn yakglplalk vwgsllhnlr ltewksaieh mknnsysgii 421 dklkisydgl epkqqemfld iacflrgeek dyilqilesc higaeyglri lidkslvfis 481 eynqvqmhdl iqdmgkyivn fqkdpgersr lwlakeveev msnntgtmam eaiwvssyss 541 tlrfsnqavk nmkrlrvfnm grssthyaid ylpnnlrcfv ctnypwesfp stfelkmlvh 601 lqlrhnslrh lwtetkhlps lrridlswsk rltrtpdftg mpnleyvnly qcsnleevhh 661 slgccskvig lylndckslk rfpcvnvesl eylglrscds leklpeiygr mkpeiqihmq 721 gsgirelpss ifqykthvtk lllwnmknlv alpssicrlk slvslsvsgc skleslpeei 781 gdldnlrvfd asdtlilrpp ssiirlnkli ilmfrgfkdg vhfefppvae glhsleylnl 841 sycnlidggl peeigslssl kkldlsrnnf ehlpssiaql galqsldlkd cqrltqlpel 901 ppelnelhvd chmalkfihy lvtkrkklhr vklddahndt mynlfaytmf qnissmrhdi 961 sasdslsltv ftgqpypeki pswfhhqgwd ssvsvnlpen wyipdkflgf avcysrslid 1021 ttahlipvcd dkmsrmtqkl alsecdtess nysewdihff fvpfaglwdt skangktpnd 1081 ygiirlsfsg eekmyglrll ykegpevnal lqmrensnep tehstgirrt qynnrtsfye 1141 ling //
Amino acid sequence for resistance protein RPP5-like from Arabidopsis thaliana :
" ORIGIN 1 mvdefqrkgv svfasedsas ddrfaeesda aiakarvsvv ifsenfassk gclneflkvs 61 kcrrskglvv vpvfygltns ivkkhclelk kmypddkvde wrnalwdiad lrgghvsshk 121 rsdselveki vadvrqkldr rgrigvysrl tkieyllckq pgciirslgi wgmagigktt 181 laraaydqls rdfeascfie dfdrefqekg ffgllekqlg vnpqvtrlsi llktlrskri 241 llvlddvrkp lgatsflcef dwlgpgslii vtsqdkqvlv qcqvneiykv qglnkheslq 301 lfsrcafgkd vpdqnllels mkfvdyangn plalsicgkn lkgktpldmk svvlelkrhl 361 sdkifvklks sydalsvsek eifldivftf rganvdnvmq slagcgffpr vgiealvdks 421 fvtvsenrvq vnnliydvgl kiindqsdei gmcyrfvdas nsqsliehke ireseqgyed 481 vkainldtsn lpfkghiafq hmynlrylti yssinptkdp dlflpgdpqf lppelrllhw 541 tcyplhsfpq nfgfqylvel nmpcsklkkl wggtknlevl kritlscsvq llnvdelqys 601 pniekidlkg clelqsfpdt gqlqhlrivd lstckkiksf pkvppsirkl hlqgtgirdl 661 sslnhssesq rltrklenvs ssnqdhrkqv lklkdsshlg slpdivifes levldfsgcs 721 elediqgfpq nlkrlylakt aikevpsslc hhisklvkld mencerlrdl pmgmsnmkyl 781 avlklsgcsn lenikelprn lkelylagta vkefpstlle tlsevvlldl enckklqglp 841 tgmskleflv mlklsgcskl eiivdlplnl ielylagtai relppsigdl alldtldlkn 901 cnrlrhlpme mhnlnplkvl dlsncselev ftsslpkvre lrpaptvmll rsklpfcffi 961 fyehrvtlsl ykarlqyipe eirwmpslkt ldlsrngfte vpvsikdfsk llslrlryce 1021 nlrslpqlpr slqllnahgc sslqlitpdf kqlpryytfs ncfglpshmv sevlanapai 1081 vecrkpqqgl enalacsfcl psptsrdskl ylqpgsstmi ilnpktrstl vgfailvevs 1141 fskdfhdtag lgfrwndkkg hahkrdnifh cwapgevvpk inddhmfvff dlkmhpsilf 1201 egdvfgilad lvvfeifpvn kqemhvgdsc titkcgvyvi ndaagsssgn tmtpqcssmd 1261 slklldgkgk krlrvnyagl kqrekalfly iacllggeka dllaqflast dfviestled 1321 lagrylidis sngevmmppl qrnfsreiih mlpastkelv smasgspcnr nndvfvsfhg 1381 kdfrkqfisd flkklvykgi ricigdkils rslinkvike ssiavvvfse nyassslcll 1441 qlmeimkcwe elgqvvmpif ykvnpsdirn qsghfgkgfk ktckktinde rqrwsraltd 1501 aasiagecsl nwasdadmie kvandirkkl isskklgkqi qrvdcdhdnp wetefnkcie 1561 rffqlpydgn hdeslvsltt ekvidmkqtl keiyqitkvk lknnlvgrrh innicfmhil 1621 yekceerssf nptslhtlyd ngipyqvytk rkkhmsgeek apiggcvgcf ymgqnqgitk 1681 agggrtmpta ypaapqanqg ynagaqpypp tayav //