This page was produced as an assignment for an undergraduate course at Davidson College

Spring 2002

Alan Cubre's Molecular Biology web page

This webpage was created to give sequence, protein information and experiments for the clonning and expression of five Saccharomyces cerevisae genes: IDH1, IDH2, IDP1, ID2 and IDP2 in E. coli.

Project 1 (Due Febuary 20)

Gene Information: fully annotated gene, amino acid sequences, accession numbers, coenzymes and all six cutter restriction sites

Gene amplification and pQE-30 UA vector: The PCR primer design and the in-frame fusion protein

Experimental Design: Is the cloned PCR product in the foward or reverse orientation?

Project 2 (Due March 18)

Project 3 (Due April 12)


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