This page was produced as an assignment for an undergraduate course at Davidson College
Alan Cubre's Molecular Biology web page
This webpage was created to give sequence, protein information and experiments for the clonning and expression of five Saccharomyces cerevisae genes: IDH1, IDH2, IDP1, ID2 and IDP2 in E. coli.
Project 1 (Due Febuary 20)
Gene Information: fully annotated gene, amino acid sequences, accession numbers, coenzymes and all six cutter restriction sites
Gene amplification and pQE-30 UA vector: The PCR primer design and the in-frame fusion protein
Experimental Design: Is the cloned PCR product in the foward or reverse orientation?
Project 2 (Due March 18)
Project 3 (Due April 12)
© Copyright 2002 Department of Biology, Davidson College, Davidson, NC 28036
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