This page was designed for an undergraduate course at Davidson College.

Our five favorite yeast genes:

IDH1, IDH2, IDP1, IDP2, IDP3

NOTE: INFORMATION POSTED BELOW WAS ASSEMBLED FROM THE SACCHROMYCES GENOME DATABASE

RUN BY STANFORD UNIVERSITY.

1. IDH1/YNL037C

Accession number: Z71313 Y13139 [ gi:1301885]

Gene sequence: coding sequence

Amino acid sequence:

MLNRTIAKRTLATAAQAERTLPKKYGGRFTVTLIPGDGVGKEITDSVRTIFEAENIPIDW
ETINIKQTDHKEGVYEAVESLKRNKIGLKGLWHTPADQTGHGSLNVALRKQLDIYANVAL
FKSLKGVKTRIPDIDLIVIRENTEGEFSGLEHESVPGVVESLKVMTRPKTERIARFAFDF
AKKYNRKSVTAVHKANIMKLGDGLFRNIITEIGQKEYPDIDVSSIIVDNASMQAVAKPHQ
FDVLVTPSMYGTILGNIGAALIGGPGLVAGANFGRDYAVFEPGSRHVGLDIKGQNVANPT
AMILSSTLMLNHLGLNEYATRISKAVHETIAEGKHTTRDIGGSSSTTDFTNEIINKLSTM*

Six-cutter restriction sites:

PCR primer:

IDH1 Forward: 5' ATGCTTAACAGAACAATTGC 3' Tm = 46° C

IDH1 Reverse: 5' TTACATGGTAGATAATTTGTTG 3' Tm = 46° C

In-frame fusion protein:

his-his-his-his-his-his-met-leu-asn-arg-thr-ile-ala-lys-arg-thr

co-enzyme: NAD+

2. IDH2/YOR136W

Accession number: Z75043 [gi:1420345]

Gene sequence: coding sequence

Amino acid sequence:

MLRNTFFRNTSRRFLATVKQPSIGRYTGKPNPSTGKYTVSFIEGDGIGPEISKSVKKIFS
AANVPIEWESCDVSPIFVNGLTTIPDPAVQSITKNLVALKGPLATPIGKGHRSLNLTLRK
TFGLFANVRPAKSIEGFKTTYENVDLVLIRENTEGEYSGIEHIVCPGVVQSIKLITRDAS
ERVIRYAFEYARAIGRPRVIVVHKSTIQRLADGLFVNVAKELSKEYPDLTLETELIDNSV
LKVVTNPSAYTDAVSVCPNLYGDILSDLNSGLSAGSLGLTPSANIGHKISIFEAVHGSAP
DIAGQDKANPTALLLSSVMMLNHMGLTNHADQIQNAVLSTIASGPENRTGDLAGTATTSS
FTEAVIKRL*

Six-cutter restriction sites:

PCR primer:

IDH2 Forward: 5' ATGTTGAGAAATACTTTTTTTTAG 3' Tm = 45° C

IDH2 Reverse: 5' TTATAATCTCTTGATGACTG 3' Tm = 44° C

In-frame fusion protein:

his-his-his-his-his-his-met-leu-arg-asn-thr-phe-phe-arg-asn-thr

co-enzyme: NAD+

3. IDP1

Accession number: Z74114 [gi:1431073]

Gene sequence: coding sequence

Amino acid sequence:

MSMLSRRLFSTSRLAAFSKIKVKQPVVELDGDEMTRIIWDKIKKKLILPYLDVDLKYYDL
SVESRDATSDKITQDAAEAIKKYGVGIKCATITPDEARVKEFNLHKMWKSPNGTIRNILG
GTVFREPIVIPRIPRLVPRWEKPIIIGRHAHGDQYKATDTLIPGPGSLELVYKPSDPTTA
QPQTLKVYDYKGSGVAMAMYNTDESIEGFAHSSFKLAIDKKLNLFLSTKNTILKKYDGRF
KDIFQEVYEAQYKSKFEQLGIHYEHRLIDDMVAQMIKSKGGFIMALKNYDGDVQSDIVAQ
GFGSLGLMTSILVTPDGKTFESEAAHGTVTRHYRKYQKGEETSTNSIASIFAWSRGLLKR
GELDNTPALCKFANILESATLNTVQQDGIMTKDLALACGNNERSAYVTTEEFLDAVEKRL
QKEIKSIE*

Six-cutter restriction sites:

PCR primer:

IDP1 Forward: 5' ATGAGTATGTTATCTAGAAG 3' Tm = 44° C

IDP1 Reverse: 5' TTACTCGATCGACTTGATTT 3' Tm = 46° C

In-frame fusion protein:

his-his-his-his-his-his-met-ser-met-leu-ser-arg-arg-leu-phe-ser

co-enzyme: NADP+

4. IDP2

Accession number: U17246 [gi:577192]

Gene sequence: coding sequence

Amino acid sequence:

MTKIKVANPIVEMDGDEQTRIIWHLIRDKLVLPYLDVDLKYYDLSVEYRDQTNDQVTVDS
ATATLKYGVAVKCATITPDEARVEEFHLKKMWKSPNGTIRNILGGTVFREPIIIPRIPRL
VPQWEKPIIIGRHAFGDQYKATDVIVPEEGELRLVYKSKSGTHDVDLKVFDYPEHGGVAM
MMYNTTDSIEGFAKASFELAIERKLPLYSTTKNTILKKYDGKFKDVFEAMYARSYKEKFE
SLGIWYEHRLIDDMVAQMLKSKGGYIIAMKNYDGDVESDIVAQGFGSLGLMTSVLITPDG
KTFESEAAHGTVTRHFRQHQQGKETSTNSIASIFAWTRGIIQRGKLDNTPDVVKFGQILE
SATVNTVQEDGIMTKDLALILGKSERSAYVTTEEFIDAVESRLKKEFEAAAL*

Six-cutter restriction sites:

PCR primer:

IDP2 Forward: 5' ATGACAAAGATTAAGGTAGC 3' Tm = 46° C

IDP2 Reverse: 5' TTACAATGCAGCTGCCTCGA 3' Tm = 52° C

In-frame fusion protein:

his-his-his-his-his-his-met-thr-lys-ile-lys-val-ala-asn-pro-ile

co-enzyme: NADP+

5. IDP3/YNL009W

Accession number: Z71285 [gi:1301827]

Gene sequence: coding sequence

Amino acid sequence:

MSKIKVVHPIVEMDGDEQTRVIWKLIKEKLILPYLDVDLKYYDLSIQERDRTNDQVTKDS
SYATLKYGVAVKCATITPDEARMKEFNLKEMWKSPNGTIRNILGGTVFREPIIIPKIPRL
VPHWEKPIIIGRHAFGDQYRATDIKIKKAGKLRLQFSSDDGKENIDLKVYEFPKSGGIAM
AMFNTNDSIKGFAKASFELALKRKLPLFFTTKNTILKNYDNQFKQIFDNLFDKEYKEKFQ
ALKITYEHRLIDDMVAQMLKSKGGFIIAMKNYDGDVQSDIVAQGFGSLGLMTSILITPDG
KTFESEAAHGTVTRHFRKHQRGEETSTNSIASIFAWTRAIIQRGKLDNTDDVIKFGNLLE
KATLDTVQVGGKMTKDLALMLGKTNRSSYVTTEEFIDEVAKRLQNMMLSSNEDKKGMCKL
*

Six-cutter restriction sites:

PCR primer:

IDP3 Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' Tm = 45° C

IDP3 Reverse: 5' TTATAGTTTGCACATACCTT 3' Tm = 44° C

In-frame fusion protein:

his-his-his-his-his-his-met-ser-lys-ile-lys-val-val-his-pro-ile

co-enzyme: NADP+

Return to the Davidson College Biology Home Page

Return to the Molecular Biology Home Page

Return to Amber's molecular home page

email the author with questions or comments: amhartman@davidson.edu

View other web pages designed by this student