Nicole Hesson's Molecular Biology Web Page

This web page was produced as an assignment for an undergraduate course at Davidson College.




The goal of this semesterís lab is to clone all five IDH genes from Saccharomyces cerevisiae using PCR and express the encoded proteins in bacteria.After using the yeast gene as a probe to see if it hybridizes any orthologs from yeast, we will perform enzyme assays to determine if the genes retain their properties when expressed in a prokaryote.  The experiment is described in more detail on the weekly protocol page


Figure 1: Wildtype W303 cells with GFP imported in mitochondria and DNA stained with dapi. 

Image provided by Teresa Rinaldi


Project 1: Describe the five IDH Genes.

IDH1

IDH2

IDP1

IDP2

IDP3

In Frame Fusion Protein

PCR Primer Design

Orientation Determination

 

Project 2: Structure and Orthologs

 

Project 3: Paper Review

 


Fantastic Resources for Yeast Information

Genbank

SGD

 


References

Molecular Biology (BIO 304), 2002. "Spring 2002 Lab Project Explained." www.bio.davidson.edu/Courses/Molbio/labcontext.html

Purves, W.K., et al. 1998. Life: The Science of Biology, 5th Ed. Sinauer Associates, MA.

 

Molecular Bio Homepage

Questions, Comments or Concerns?

Email Nicole Hesson