This web page was created for an undergraduate molecular biology course at Davidson College
Digest with Eco RV
This will cut 16bp's upstream of the insert, and in the insert at bp 340. The IDH1 gene is 1083 bp long.
Correct orientation fragment is 356 bp. (16+340)
Incorrect orientation fragment is 759 bp. (16+743)
Digest with Eco RV
This will cut 16bp's upstream of the insert, and in the insert at bp 790. The IDH2 gene is 1110 bp long.
Correct orientation fragment is 806 bp. (16+790)
Incorrect orientatino fragment is 336 bp. (16+320)
Digest with Bam HI
This will cut 29 bp's upstream of the insert, and in the insert at bp 780. The IDP1 gene is 1287 bp long.
Correct orientation fragment is 809 bp. (29+780)
Incorrect orientatino fragment is 536 bp. (29+507)
Digest with Bgl II
This will cut 15 bp downstream of the insert, and in the insert at bp 496 (careful, we are cutting downstream so watch your adding!). The IDP2 gene is 1239 bp long.
Correct orientation fragment will be 758 bp. (15+743)
Incorrect orientation fragment will be 511 bp. (496 + 15)
Digest with both Cla I and Eco RV
Eco RV will cut 15 bp upstream of the insert, and Cla I will but in the insert at bp 495. The IDP3 gene is 1263 bp long.
Correct orientation fragment will be 511 bp. (16+495)
Incorrect orientation will be 784 bp. (16+768)
Contact Kevin James with comments or questions.