This web page was created for an undergraduate molecular biology course at Davidson College

In-Frame Fusion Proteins of His6+IDH

There are three main pieces of the fusion protein to note:

1) The RGS-His epitope

2) The Polylinker

3) First 10 amino acids of the IDx gene

IDH1

5' RGSHHHHHHGSHVISSIASMLNRTIAKRT... 3'

IDH2

5' RGSHHHHHHGSHVISSIASMLRNTFFRNT...3'

IDP1

5' RGSHHHHHHGSHVISSIASMSMLSRRLFS...3'

IDP2

5' RGSHHHHHHGSHVISSIASMTKIKVANPI...3'

IDP3

5' RGSHHHHHHGSHVISSIASMSKIKVVHPI...3'

Helpful Hints:

• View Amino Acid Abbreviation Key
• View our Expression Vector pQE-30 UA

Contact Kevin James with comments or questions.

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