This web page was created for an undergraduate molecular biology course at Davidson College

Kevin's PCR Primer Sequences

All Melting Temps are between 44-46 C

IDH1:

Forward: 5' ATGCTTAACAGAACAATTGC 3'

Reverse: 5' TTACATGGTAGATAATTTGTTG 3'

IDH2:

Forward: 5' ATGTTGAGAAATACTTTTTTTAG 3'

Reverse: 5' TTATAATCTCCTTCAGTGAA 3'

IDP1:

Forward: 5' ATGAGTATGTTATCTAGAAG 3'

Reverse: 5' TTACTCGATCGACTTGATTT 3'

IDP2:

Forward: 5' ATGACAAAGATTAAGGTAGC 3'

Reverse: 5' TTACAATGCAGCTGCCT 3'

IDP3:

Forward: 5' ATGAGTAAAATTAAAGTTGTTC 3'

Reverse: 5' TTATAGTTTACACTGTCTTC 3'

 

Check The Actual Primers Used by Class

Check Out Our Results (Chris Norbet and I are cloning IDH1 on Thursday afternoon)

 

Contact Kevin James with comments or questions.

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