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Nucleotide Sequence
1 ATGCTTAACA GAACAATTGC TAAGAGAACT TTAGCCACTG CCGCTCAGGC
51 GGAACGCACC CTACCCAAGA AGTATGGCGG TCGTTTCACC GTCACTTTGA
101 TACCTGGTGA CGGTGTTGGG AAAGAAATCA CTGATTCAGT GAGAACCATT
151 TTTGAGGCTG AAAATATCCC GATCGACTGG GAAACTATAA ACATTAAGCA
201 AACAGATCAT AAGGAAGGCG TCTATGAAGC TGTTGAGTCT CTAAAGAGAA
251 ATAAGATTGG TCTTAAGGGG CTATGGCACA CTCCTGCTGA CCAAACAGGT
301 CACGGTTCAC TAAACGTTGC TTTGCGTAAA CAACTAGATA TCTACGCCAA
351 TGTGGCCCTT TTCAAATCCT TGAAGGGTGT CAAGACTAGA ATTCCAGACA
401 TAGATTTGAT TGTCATTAGA GAAAACACGG AGGGTGAGTT CTCAGGCCTG
451 GAACATGAAT CCGTCCCTGG TGTAGTGGAA TCTTTGAAAG TTATGACTAG
501 ACCTAAGACA GAAAGGATCG CCAGATTTGC CTTTGACTTC GCCAAGAAAT
551 ACAACAGAAA GTCTGTCACA GCTGTGCATA AGGCAAATAT CATGAAGTTA
601 GGTGACGGTC TGTTCAGAAA TATAATAACT GAAATTGGCC AAAAAGAATA
651 TCCTGATATT GACGTATCGT CCATCATTGT CGACAATGCC TCCATGCAGG
701 CGGTGGCCAA ACCTCATCAA TTTGATGTCC TAGTTACCCC TTCAATGTAC
751 GGTACCATCT TAGGCAACAT TGGCGCTGCT TTGATCGGTG GTCCAGGATT
801 GGTGGCAGGT GCCAACTTTG GCAGGGACTA TGCTGTCTTC GAACCAGGTT
851 CCAGACATGT TGGTTTAGAT ATTAAAGGCC AAAATGTGGC TAACCCAACT
901 GCCATGATCC TTTCCTCCAC GTTAATGTTG AACCATTTGG GTTTGAATGA
951 ATATGCTACT AGAATCTCAA AGGCAGTTCA TGAAACGATC GCAGAAGGTA
1001 AGCATACCAC TAGAGATATT GGTGGTTCCT CTTCTACTAC TGACTTCACG
1051 AATGAAATCA TCAACAAATT ATCTACCATG TAA
Amino Acid Sequence
1 MLNRTIAKRT LATAAQAERT LPKKYGGRFT VTLIPGDGVG KEITDSVRTI
51 FEAENIPIDW ETINIKQTDH KEGVYEAVES LKRNKIGLKG LWHTPADQTG
101 HGSLNVALRK QLDIYANVAL FKSLKGVKTR IPDIDLIVIR ENTEGEFSGL
151 EHESVPGVVE SLKVMTRPKT ERIARFAFDF AKKYNRKSVT AVHKANIMKL
201 GDGLFRNIIT EIGQKEYPDI DVSSIIVDNA SMQAVAKPHQ FDVLVTPSMY
251 GTILGNIGAA LIGGPGLVAG ANFGRDYAVF EPGSRHVGLD IKGQNVANPT
301 AMILSSTLML NHLGLNEYAT RISKAVHETI AEGKHTTRDI GGSSSTTDFT
351 NEIINKLSTM
Accession Number
6323989
Primers
Forward: 5' ATGCTTAACAGAACAATTGC 3' Tm = 46° C
Reverse: 5' TTACATGGTAGATAATTTGTTG 3' Tm = 46° C
Coenzyme
NAD+
In-frame Fusion Proteins of 6xHis
MRGS-HHHHHH-GSHVISSIVS-MLNRTIAKRTLAT
Six-cutter Restriction Sites
Six-base cutter enzymes that do not cut:
AatII AfeI AgeI ApaI ApaLI AseI AvaI AvrII BamHI BanII BbeI BclI BfrBI BglII Bme1580I BsaAI BsaWI BsiHKAI BsiWI Bsp1286I BspEI BsrFI BsrGI BssHII BssSI BstYI BstZ17I BtgI BtrI ClaI DraI EagI Ecl136II FspI HindIII HpaI KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PmlI Ppu10I PsiI PspOMI PstI SacI SacII ScaI SfcI SfoI SmaI SnaBI SpeI SphI SspI StyI TatI XbaI XhoI XmaI ZraI
