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Nucleotide Sequence
1 ATGAGTAAAA TTAAAGTTGT TCATCCCATC GTGGAAATGG ACGGTGATGA
51 GCAGACAAGA GTTATTTGGA AACTTATCAA AGAAAAATTG ATATTGCCAT
101 ATTTAGATGT GGATTTAAAA TACTATGACC TTTCAATCCA AGAGCGTGAT
151 AGGACTAATG ATCAAGTAAC AAAGGATTCT TCTTATGCTA CCCTAAAATA
201 TGGGGTTGCT GTCAAATGTG CCACTATAAC ACCCGATGAG GCAAGAATGA
251 AAGAATTTAA CCTTAAAGAA ATGTGGAAAT CTCCAAATGG AACAATCAGA
301 AACATCCTAG GTGGAACTGT ATTTAGAGAA CCCATCATTA TTCCAAAAAT
351 ACCTCGTCTA GTCCCTCACT GGGAGAAACC TATAATTATA GGCCGTCATG
401 CTTTTGGTGA CCAATATAGG GCTACTGACA TCAAGATTAA AAAAGCAGGC
451 AAACTAAGGT TACAGTTTAG CTCAGATGAC GGTAAAGAAA ACATCGATTT
501 AAAGGTTTAT GAATTTCCTA AAAGTGGTGG GATCGCAATG GCAATGTTTA
551 ATACAAATGA TTCCATTAAA GGGTTCGCAA AGGCATCCTT CGAATTAGCT
601 CTCAAAAGAA AACTACCGTT ATTCTTTACA ACCAAAAACA CTATTCTGAA
651 AAATTATGAT AATCAGTTCA AACAAATTTT CGATAATTTG TTCGATAAAG
701 AATATAAGGA AAAGTTTCAG GCTTTAAAAA TAACGTACGA GCATCGTTTG
751 ATTGATGATA TGGTAGCACA GATGCTAAAA TCAAAGGGCG GGTTTATAAT
801 CGCCATGAAG AATTATGATG GCGATGTCCA GTCTGACATT GTGGCACAAG
851 GATTTGGGTC TCTTGGTTTA ATGACGTCCA TATTGATTAC ACCTGATGGT
901 AAAACGTTTG AAAGCGAGGC TGCCCATGGT ACGGTGACCA GACATTTTAG
951 AAAACATCAA AGAGGCGAAG AAACATCAAC AAATTCAATA GCCTCAATAT
1001 TTGCCTGGAC AAGGGCAATT ATACAAAGAG GAAAATTAGA CAATACAGAT
1051 GATGTTATAA AATTTGGAAA CTTACTAGAA AAGGCTACTT TGGACACAGT
1101 TCAAGTGGGC GGAAAAATGA CCAAGGATTT AGCATTGATG CTTGGAAAGA
1151 CTAATAGATC ATCATATGTA ACCACAGAAG AGTTTATTGA TGAAGTTGCC
1201 AAGAGGCTTC AAAACATGAT GCTCAGCTCC AATGAAGACA AGAAAGGTAT
1251 GTGCAAACTA TAA
Amino Acid Sequence
1 MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD
51 RTNDQVTKDS SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR
101 NILGGTVFRE PIIIPKIPRL VPHWEKPIII GRHAFGDQYR ATDIKIKKAG
151 KLRLQFSSDD GKENIDLKVY EFPKSGGIAM AMFNTNDSIK GFAKASFELA
201 LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ ALKITYEHRL
251 IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG
301 KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD
351 DVIKFGNLLE KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA
401 KRLQNMMLSS NEDKKGMCKL
Accession Number
6323989
Primers
Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' Tm = 45° C
Reverse: 5' TTATAGTTTGCACATACCTT 3' Tm = 44° C
Coenzyme
NADP+
In-frame Fusion Proteins of 6xHis
MRGS-HHHHHH-GSHVISSIVS-MSKIKVVHPIVEM
Six-cutter Restriction Sites
Six-base cutter enzymes that do not cut:
AccI Acc65I AfeI AflII AflIII AgeI ApaI ApaLI AseI AvaI BamHI BanI BanII BbeI BfrBI BglII Bme1580I BsaAI BsaWI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstYI BstZ17I BtrI EaeI EagI Ecl136II EcoRI EcoRV FspI HaeII HincII HindIII HpaI Hpy8I KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI NlaIV NruI NsiI NspI PciI PmlI Ppu10I PspOMI PstI PvuI PvuII SacI SacII SalI ScaI SfcI SfoI SmaI SmlI SnaBI SpeI SphI StuI TatI XbaI XhoI XmaI
