*This page is part of undergraduate assignment for Molecular Biology at Davidson College

 

 

Cloning 5 Isoforms of Isocitrate Dehydrogenase

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By Elizabeth Shafer                                     

Orthologs 

 

I did a BLASTp search of the first 40 amino acids of IDP2 to search for amino acid structural homologs. Below, I have described the nucleotide sequence, amino acid sequence, and reproduced a sequence alignment amongst five IDP2 orthologs.

 

Isocitrate Dehydrogenase from E.coli (IBL5).

Escherichia coli is enterobacteria. Its has a gene that encodes for isocitrate dehydrogenase.

For more information check:

Hurley JH, Thorsness PE, Ramalingam V, Helmers NH, Koshland DE Jr, Stroud RM.Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8635-9.

 

         Nucleotide Sequence CDS  1251 bp
                                                          1 atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa
                                                                            61 ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat
                                                                            121 gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag
                                                                            181 cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag
                                                                            241 gacgtctggc tgcctgctga aactcttgat ctgattcgtg aatatcgcgt tgccattaaa
                                                                            301 ggtccgctga ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag
                                                                            361 gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg
                                                                            421 gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat
                                                                            481 gcgggtatcg aatggaaagc agactctgcc gacgccgaga aagtgattaa attcctgcgt
                                                                            541 gaagagatgg gggtgaagaa aattcgcttc ccggaacatt gtggtatcgg tattaagccg
                                                                             601 tgttcggaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac
                                                                            661 gatcgtgact ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggagcg
                                                                            721 tttaaagact ggggctacca gctggcgcgt gaagagtttg gcggtgaact gatcgacggt
                                                                            781 ggcccgtggc tgaaagttaa aaacccgaac actggcaaag agatcgtcat taaagacgtg
                                                                            841 attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc
                                                                            901 tgtatgaacc tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc
                                                                            961 ggtatcgccc ctggtgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt
                                                                            1021 actgcgccga aatatgccgg tcaggacaaa gtaaatcctg gctctattat tctctccgct
                                                                            1081 gagatgatgc tgcgccacat gggttggacc gaagcggctg acttaattgt taaaggtatg
                                                                            1141 gaaggcgcaa tcaacgcgaa aaccgtaacc tatgacttcg agcgtctgat ggatggcgct
                                                                            1201 aaactgctga aatgttcaga gtttggtgac gcgatcatcg aaaacatgta a
 
               Amino Acid Sequence 416 aa 
                                                             MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                                                                             MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                                                                             LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                                                                            AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                                                                            SVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                                                                            IADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                                                                            FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                                                                            FERLMDGAKLLKCSEFGDAIIENM
Isocitrate Dehydrogenase from Bacillus coagulans (2AYQ_B)

Bacillus coagulans is a bacillus bacteria and moderate thermophile. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis at high temperatures.

For more information check:
Tsuchiya D, Sekiguchi T, Takenaka A.Crystal structure of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile, Bacillus coagulans: two strategies for thermostabilization of protein structures.

J Biochem (Tokyo). 1997 Dec;122(6):1092-104.
            
            CDS 1101 bp
                                                                            1 atgaaaatga aactggccgt actgcccggc gatgggatcg ggccggaagt gatggatgca
                                                                            61 gcgatccgcg ttttaaaaac agtgttggac aatgacgggc atgaagccgt ttttgaaaat
                                                                            121 gcgctgattg ggggcgccgc cattgatgaa gcggggacgc ccctaccgga agaaacgctt
                                                                            181 gacatttgcc gcaggagcga tgccattttg ctcggcgcgg taggggggcc gaaatgggat
                                                                            241 cataacccgg cttccctccg cccggaaaaa ggcctgctcg ggctccggaa agaaatgggg
                                                                            301 ctgtttgcga acctgcgccc ggttaaagca tatgccacac ttttaaacgc atcgccttta
                                                                            361 aaacgggaac gtgtggaaaa cgtcgatctt gttattgtcc gcgaactgac gggcggcctc
                                                                            421 tattttgggc gcccgagtga aaggcgcggg ccgggcgaga atgaagtggt agacacgctt
                                                                            481 gcctatacaa gggaagagat tgaaagaatt attgagaaag cattccagct tgcccaaatc
                                                                            541 agaagaaaaa aactggcatc cgtcgataag gcgaatgtgc tggaatcaag cagaatgtgg
                                                                            601 cgcgaaattg cggaagaaac cgcgaaaaag tatccggacg tggaattgag ccatatgctt
                                                                            661 gtcgactcaa cttcgatgca gctgattgca aatccgggcc aatttgatgt cattgtaaca
                                                                            721 gagaatatgt tcggcgatat tttaagcgat gaagcgtccg tgattaccgg cagcctcggc
                                                                            781 atgttgccat ccgcaagcct ccgttccgac cggttcggca tgtatgaacc ggtccacggc
                                                                            841 tccgcgccgg atattgccgg gcagggaaaa gccaacccgc tcgggacagt gctgtcagcg
                                                                            901 gctttgatgc tccgttattc gttcgggctt gagaaagaag cggcggccat tgaaaaagca
                                                                            961 gtggatgatg tgcttcaaga cggctattgt acaggcgatt tgcaggtggc aaacggaaaa
                                                                            1021 gtggtcagta caattgagct cacagaccgg ctgatcgaaa aattaaataa cagcgcagcc
                                                                            1081 ggtccgcgca tttttcaata a
               Amino Acid Sequence 336 aa
                                                                            1 mkmklavlpg dgigpevmda airvlktvld ndgheavfen aliggaaide agtplpeetl
                                                                            61 dicrrsdail lgavggpkwd hnpaslrpek gllglrkemg lfanlrpvka yatllnaspl
                                                                            121 krervenvdl vivreltggl yfgrpserrg pgenevvdtl aytreeieri iekafqlaqi
                                                                            181 rrkklasvdk anvlessrmw reiaeetakk ypdvelshml vdstsmqlia npgqfdvivt
                                                                             241 enmfgdilsd easvitgslg mlpsaslrsd rfgmyepvhg sapdiagqgk anplgtvlsa
                                                                            301 almlrysfgl ekeaaaieka vddvlqdgyc tgdlqvangk vvstieltdr lieklnnsaa
                                                                            361 rprifq
Isocitrate Dehydrogenase from Thermus thermophilus (1XAA).

Thermus thermophilus is an extremophile. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis at high temperatures.

For more information check:

 Yamada T, Akutsu N, Miyazaki K, Kakinuma K, Yoshida M, Oshima T.Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8.
J Biochem (Tokyo). 1990 Sep;108(3):449-56.

 
 
            CDS 1488 bp
                                              1 atgcccctga tcaccacgga aaccggcaag aagatgcacg ttctcgagga cgggcgcaag
                                              61 ctcatcaccg tcatccccgg agacggcatc gggcccgagt gcgtggaggc taccctcaag
                                              121 gtcctagagg cggccaaggc ccccctggcc tacgaggtgc gagaggcggg ggcgagcgtc
                                              181 ttccggcggg gcatcgcctc gggcgttccc caggagacca ttgagtccat ccgcaagacc
                                               241 cgggtggtcc tgaagggtcc cctggaaacc ccggtgggct acggggagaa gagcgccaac
                                              301 gtcaccctaa ggaagctctt tgagacctac gccaacgtcc gccccgtgcg ggagttcccc
                                              361 aacgtcccca ccccctatgc gggccggggc attgacctcg tggtggtgcg ggagaacgtg
                                              421 gaggacctct acgccgggat tgagcacatg cagaccccga gcgtggccca gaccctcaag
                                              481 ctcatctcct ggaagggatc ggagaagatc gtccgcttcg cctttgagct ggcccgggcc
                                              541 gaggggcgga agaaggtcca ctgcgccacc aagtccaaca tcatgaagct cgccgaagga
                                              601 cccaagcggg cctttgagca ggtggcccag gagtaccccg acatagaagc ggtccacatc
                                              661 atcgtggaca acgctgccca ccagctggtg aaaaggcccg agcagtttga ggtgatcgtc
                                              721 accaccaaca tgaacggaga catcctctcc gacctcacct cggggctcat tgggggcctg
                                              781 ggcttcgctc cctcggccaa catcggcaac gaggtggcca tctttgaggc cgtccacggt
                                              841 tccgccccca agtacgccgg gaagaacgtc atcaacccca ccgcggtcct cctctcggcg
                                              901 gtgatgatgc tccgctacct ggaggagttc gccacggcgg accttataga gaacgccctc
                                              961 ctctacaccc tcgaggaggg ccgggtcctc acgggggacg tggtgggcta cgaccggggg
                                              1021 gccaagacca cggagtacac cgaggccatc atccagaacc tgggcaagac cccaaggaag
                                              1081 acccaggtgc ggggctacaa gcccttccgc ctgccccagg tggacggggc catcgccccc
                                              1141 atcgtcccta ggagccgccg ggttgtgggg gtggacgtct tcgtggaaac caacctcctg
                                              1201 cccgaggccc tgggaaaggc cctggaggac cttgccgcgg gcaccccctt ccggctcaag
                                              1261 atgatctcca accggggcac ccaggtctac ccccccaccg gcgggctcac ggacctggtg
                                              1321 gaccactacc gctgccgctt cctctacacg ggggaggggg aggctaagga cccggagatc
                                              1381 ctggacctcg taagccgggt ggcaagccgc ttccgctgga tgcacctgga gaagctccag
                                              1441 gaatttgacg gcgagcccgg cttcaccaag gcccaagggg aagactaa
            Amino Acid Sequence 345 aa
                                     1 mkvavlpgdg igpevteaal kvlraldeae glglayevfp fggaaidafg epfpeptrkg
                                              61 veeaeavllg svggpkwdgl prkirpetgl lslrksqdlf anlrpakvfp glerlsplke
                                              121 eiargvdvli vreltggiyf geprgmseae awnteryskp evervarvaf eaarkrrkhv
                                              181 vsvdkanvle vgefwrktve evgrgypdva lehqyvdama mhlvrsparf dvvvtgnifg
                                              241 dilsdlasvl pgslgllpsa slgrgtpvfe pvhgsapdia gkgianptaa ilsaammleh
                                              301 afglvelark vedavakall etpppdlggs agteaftatv lrhla
Isocitrate Dehydrogenase from Brassica napus (gi 126201)
Brassica napus is a flowering plant.  Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is a chloroplast precusor. 
            For more information check: 
Ellerstrom M, Josefsson LG, Rask L, Ronne H.Cloning of a cDNA for rape chloroplast 3-isopropylmalate dehydrogenase by genetic complementation in yeast.

Plant Mol Biol. 1992 Feb;18(3):557-66.
 
               CDS 1221 bp
                               1 atggcggcgg ctctgcagac taacatccga ccggttaagt ttccggctac gttgagagct
                               61 ctcaccaaac aatcttctcc agcacccttt agagtgagat gcgccgctgc ttcccccggg
                               121 aaaaagagat acaatatcac tctccttccc ggcgatggaa tcggtccgga ggtcatctcc
                               181 atcgctaaaa atgtgcttca gcaagctggt tccttggaag gtctggagtt tagcttccag
                               241 gaaatgcctg taggaggagc tgctttggat ttggtcggag tgcctttgcc tgaggagacc
                               301 gtctcggctg ctaaagaatc agatgctgtg cttcttggag ccattggagg gtacaaatgg
                               361 gataagaatg aaaaacattt gaagcctgag actgggttac ttcaacttcg ggctggtctt
                               421 aaagtctttg ctaatctgag acctgctaca gttcttccac agttagtgga tgcttcgacc
                               481 ttgaagagag aggttgcaga aggtgttgat ctgatggttg ttagggagct tacaggaggt
                               541 atttactttg gagtgccaag gggcattaag actaatgaaa atggtgagga agttgggtat
                               601 aataccgagg tctatgctgc tcacgagatt gatagaattg ctcgtgttgc cttcgagact
                               661 gctcggaaac ggcgtggcaa gctgtgttct gttgacaaag ctaatgtctt agatgcctcg
                               721 attttatgga ggagacgagt aacagcacta gctgctgaat atccggatgt tgaactgtca
                               781 catatgtatg ttgacaatgc tgccatgcag cttgttcgtg accctaaaca gtttgacacc
                               841 attgttacaa acaacatttt tggtgatata ttatccgatg aagcgtcgat gatcacagga
                               901 agcatcggca tgcttccctc tgctagtctc agtgattcgg gacctggact ctttgaacct
                               961 atacatggtt ctgcacctga tattgctgga caggataaag caaacccgtt ggcaaccatc
                               1021 ctcagcgctg caatgcttct gaaatacgga ctcggagagg agaaggcagc taagagaatc
                               1081 gaagacgctg tgttgggtgc tctgaacaaa ggattcagaa caggagacat ctactccgca
                               1141 ggaactaaac ttgtgggctg caaggagatg ggagaggaag ttctgaagtc agtggattcc
                               1201 cacgttcaag cttctgttta a
            Amino Acid Sequence 406 aa
                               1 maaalqtnir pvkfpatlra ltkqsspapf rvrcaaaspg kkrynitllp gdgigpevis
                               61 iaknvlqqag sleglefsfq empvggaald lvgvplpeet vsaakesdav llgaiggykw
                               121 dknekhlkpe tgllqlragl kvfanlrpat vlpqlvdast lkrevaegvd lmvvreltgg
                               181 iyfgvprgik tnengeevgy ntevyaahei driarvafet arkrrgklcs vdkanvldas
                               241 ilwrrrvtal aaeypdvels hmyvdnaamq lvrdpkqfdt ivtnnifgdi lsdeasmitg
                               301 sigmlpsasl sdsgpglfep ihgsapdiag qdkanplati lsaamllkyg lgeekaakri
                               361 edavlgalnk gfrtgdiysa gtklvgckem geevlksvds hvqasv
Isocitrate Dehydrogenase from Neurospora crassa  (gi_462502)
Neurospora crassa is an eukaryotic fungi.  Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis. 
For more information check: 

Li Q, Jarai G, Yaghmai B, Marzluf GA. The leu-1 gene of Neurospora crassa: nucleotide and deduced amino acid sequence comparisons. Gene 1993 Dec 22;136(1-2):301-5
 
               CDS 1107 bp
                               1 atggctactc ataacattgt tgtgttcggt ggtgaccact gcggtcccga ggttgttctc
                               61 gaggccatca aggtcctcaa ggcgatcgag accaacagcc cttcggcgtg caagttcaac
                               121 ctccagaacc acctccttgg cggtgcctcc atcgacaagc acaatgaccc cctcaccgat
                               181 gaggccctca acgccgccaa ggctgccgat gccgtccttc tcggtgccat tggcggtccc
                               241 gaatggggca cctcttccac cgtccgcccc gagcaaggtc tcctgaagct ccgcaaggag
                               301 ctcggcacct atggcaacct tcgcccttgc aactttgctt ccgagtccct cgtcgacagc
                               361 tctcccctca aggccgaggt ctgccgcggc actgacttca ttgtcgtccg tgagcttacc
                               421 ggtggtatct actttggtga ccgcaccgag gatgacggct ccggctacgc ctgcgatacc
                               481 gagccctaca gccgcgccga gatcgtgcgc atcgccagac tcgccggctt cctcgccctg
                               541 gccaagaact ctcccgccaa ggtctggtct ctggacaagg ccaacgtgct cgccaccagc
                               601 cgcctctggc gcaagactgt gaccgacgtc attagcaagg agtgccccca gcttcagctc
                               661 gagcaccagc tcatcgacag cgccgccatg ctgctcgtca agaacccccg tgccctcaac
                               721 ggcgtcgtca ttaccagcaa cctctttggc gacatcatct cggacgaggc ctcggtcatc
                               781 cccggctcca tcggcctgct cccttccgcc agctggggcg gaatccccga cggcaaggtc
                               841 aagtgcaacg gcatttacga gcccatccac ggctccgctc ccgatatttc gggtaagggc
                               901 atcgtcaacc ccgtctgtac cattctctcc gtcgccatga tgctccgcta ctcgctcaac
                               961 ctccccaagg aggccgatgc cgttgaggct gctgtcaagg cagccattga caacggtacc
                               1021 aagaccaagg accttggcgg caacgctact acttcggata tgggtaacgc tgtagttgct
                               1081 gagttggaga agatccttaa ggcttaa
               Amino Acid Sequence 368 bp
                               1 mathnivvfg gdhcgpevvl eaikvlkaie tnspsackfn lqnhllggas idkhndpltd
                              61 ealnaakaad avllgaiggp ewgtsstvrp eqgllklrke lgtygnlrpc nfaseslvds
                               121 splkaevcrg tdfivvrelt ggiyfgdrte ddgsgyacdt epysraeivr iarlagflal
                               181 aknspakvws ldkanvlats rlwrktvtdv iskecpqlql ehqlidsaam llvknpraln
                               241 gvvitsnlfg diisdeasvi pgsigllpsa swggipdgkv kcngiyepih gsapdisgkg
                               301 ivnpvctils vammlrysln lpkeadavea avkaaidngt ktkdlggnat tsdmgnavva
                               361 elekilka
 
Amino Acid Sequence Alignment:
 

                      10        20        30        40        50        60
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus   1 KIALLPGDGIGPEVTEAALKVLKAALEKAP---LEFEFEEYLVGGAAIDATG--EPLPDE 55
1BL5       26 IIPYIEGDGIGVDVTPAMLKVVDAAVEKAYkgeRKISWMEIYTGEKSTQVYGqdVWLPAE 85
2AYQ_B      4 KLAVLPGDGIGPEVMDAAIRVLKTVLDNDG---HEAVFENALIGGAAIDEAG--TPLPEE 58
1XAA        2 KVAVLPGDGIGPEVTEAALKVLRALDEAEG---LGLAYEVFPFGGAAIDAFG--EPFPEP 56
gi 126201  45 NITLLPGDGIGPEVISIAKNVLQQAGSLEG---LEFSFQEMPVGGAALDLVG--VPLPEE 99
gi 462502   5 NIVVFGGDHCGPEVVLEAIKVLKAIETNSPs-aCKFNLQNHLLGGASIDKHN--DPLTDE 61
                      70        80        90       100       110       120
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus  56 TLEACRKADAVLKGAVGGPKWd--pGEVRPENglLALRKELDLYANLRPVKVYp-aLGDK 112
1BL5       86 TLDLIREYRVAIKGPLTTPVG----GGIRSLN--VALRQELDLYICLRPVRYY---QGTP 136
2AYQ_B     59 TLDICRRSDAILLGAVGGPKwdhnpASLRPEKglLGLRKEMGLFANLRPVKAYa-tLLNA 117
1XAA       57 TRKGVEEAEAVLLGSVGGPKwdglpRKIRPETglLSLRKSQDLFANLRPAKVFp-gLERL 115
gi 126201 100 TVSAAKESDAVLLGAIGGYKwdkneKHLKPETglLQLRAGLKVFANLRPATVLp-qLVDA 158
gi 462502  62 ALNAAKAADAVLLGAIGGPEWgt-sSTVRPEQglLKLRKELGTYGNLRPCNFAsesLVDS 120
                     130       140       150       160       170       180
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 113 SPLKNEvvEGVDIVIVRELTGGIYFGIEKGIDG-----------------SGN-GEEVAV 154
1BL5      137 SPVKHP--ELTDMVIFRENSEDIYAGIEWKADSadaekvikflreemgvkKIRfPEHCGI 194
2AYQ_B    118 SPLKRErvENVDLVIVRELTGGLYFGRPSER-------------------RGPgENE-VV 157
1XAA      116 SPLKEEiaRGVDVLIVRELTGGIYFGEPRGMS-----------------------EAEAW 152
gi 126201 159 STLKREvaEGVDLMVVRELTGGIYFGVPRGIKT-----------------NEN-GEEVGY 200
gi 462502 121 SPLKAEvcRGTDFIVVRELTGGIYFGDRTEDDG-----------------SG-----YAC 158
                     190       200       210       220       230       240
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 155 DTKLYSRDEIERIARAAFELARKRG-RKKVTSVDKANVLKSSD----LWREIVAEVAAK- 208
1BL5      195 GIKPCSEEGTKRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEgafkDWGYQLAREEFGg 253
2AYQ_B    158 DTLAYTREEIERIIEKAFQLAQIR--RKKLASVDKANVLESSR----MWREIAEETAK-- 209
1XAA      153 NTERYSKPEVERVARVAFEAARKR--RKHVVSVDKANVLEVGE----FWRKTVEEVGR-- 204
gi 126201 201 NTEVYAAHEIDRIARVAFETARKR--RGKLCSVDKANVLDASI----LWRRRVTALAA-- 252
gi 462502 159 DTEPYSRAEIVRIARLAGFLALAKNsPAKVWSLDKANVLATSR----LWRKTVTDVISK- 213
                     250       260       270       280       290       300
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 209 ---------------EYPDIELEHMLVDNAAMQLVKNP-KQFDVIVTPNLFGDILSDEAS 252
1BL5      254 elidggpwlkvknpnTGKEIVIKDVIADAFLQQILLRP-AEYDVIACMNLNGDYISDALA 312
2AYQ_B    210 ---------------KYPDVELSHMLVDSTSMQLIANP-GQFDVIVTENMFGDILSDEAS 253
1XAA      205 ---------------GYPDVALEHQYVDAMAMHLVRSP-ARFDVVVTGNIFGDILSDLAS 248
gi 126201 253 ---------------EYPDVELSHMYVDNAAMQLVRDP-KQFDTIVTNNIFGDILSDEAS 296
gi 462502 214 ---------------ECPQLQLEHQLIDSAAMLLVKNPrALNGVVITSNLFGDIISDEAS 258
                     310       320       330       340       350       360
              ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 253 MLTGSLGMLPSASLGPDGf------ALFEPVHGSAPDIAGKDKANPIATILSAAMMLRHs 306
1BL5      313 AQVGGIGIAPGANIGDEC-------ALFEATHGTAPKYAGQDKVNPGSIILSAEMMLRH- 364
2AYQ_B    254 VITGSLGMLPSASLRSdr------fGMYEPVHGSAPDIAGQGKANPLGTVLSAALMLRYs 307
1XAA      249 VLPGSLGLLPSASLGrg-------tPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHa 301
gi 126201 297 MITGSIGMLPSASLSDsg------pGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYg 350
gi 462502 259 VIPGSIGLLPSASWGGIPdgkvkcnGIYEPIHGSAPDISGKGIVNPVCTILSVAMMLRYs 318
                     370       380       390       400
              ....*....|....*....|....*....|....*....|....*..
consensus 307 LGLED-AaDAIEAAVLKTLEAGIRTKDLAGNAD--KYVSTSEFGDAV 350
1BL5      365 MGWTEaA-DLIVKGMEGAINAKTVTYDFERLMDgaKLLKCSEFGDAI 410
2AYQ_B    308 FGLEK-EaAAIEKAVDDVLQDGYCTGDLQVANG--KVVSTIELTDRL 351
1XAA      302 FGLVE-LaRKVEDAVAKALLETP-PPDLGGSAG--TEAFTATVLRHL 344
gi 126201 351 LGEEK-AaKRIEDAVLGALNKGFRTGDIYSAGT--KLVGCKEMGEEV 394
gi 462502 319 LNLPK-EaDAVEAAVKAAIDNGTKTKDLGGNA------TTSDMGNAV 358

 


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Created by: Elizabeth Shafer. Email questions to lishafer@davidson.edu