*This page is part of undergraduate assignment for Molecular Biology at Davidson College

Cloning 5 Isoforms of Isocitrate Dehydrogenase

By Elizabeth Shafer

Structure

Image from http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?form=6&db=t&Dopt=s&uid=10337. (If image is broken go to this link.)

Isocirate Dehydrogenase in complex with NADP+ substrate from E. coli. For the best view click on the molecule and select color, chain.
Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM.Regulation of an enzyme by phosphorylation at the active site. Science. 1990 Aug 31;249(4972):1012-6.

Image from http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?form=6&db=t&Dopt=s&uid=10337 (If image is broken go to this link.)

Crystallized structure of a mutant (M219a) Thermus thermophilus.

Wallon G, Kryger G, Lovett ST, Oshima T, Ringe D, Petsko GA.Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart fromThermus thermophilus.J Mol Biol. 1997 Mar 14;266(5):1016-31.

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Created by: Elizabeth Shafer. Email questions to lishafer@davidson.edu