3D Structure of Phospholamban

CPK Color Scheme

What is that molecule on the left?? Follow this tutorial to find out about some of the cooler structural features of Phospholamban!!!

Click here to reset the image.

Let's first see the backbone of Phospholamban to get a better idea of its basic structure. Click here. Please note the alpha helix structure. The section of the protein that is depicted as horizontal in this representation is cytoplasmic and the vertical section is located in the membrane.

Click here to see the "zipper" in phospholamban . The zipper is located in the membrane region of the protein. Leucine is highlighted in green and isoleucine is highlighted in yellow. The zipper stabilizes the left-handed coiled coil of the 5 helices of pentameric phospholamban.

The zipper is located on one face of the protein, and the other side of the protein interacts with Ca(2+)-ATPase. Phosphorylation by cAMP-dependent protein kinase (PKA) disrupts the pentameric structure and forces phospholamban to its monomeric structure. Click here to see the site of phosphorylation . Click here to get a closer look at Serine16 (highlighted pink). Notice that the binding site is in a critical region between the membrane and cytoplasmic regions of the protein.

Kogoy, J. (2003). CPK Color Scheme. Permission to use granted.

Mortishire-Smith RJ, Pitzenberger SM, Burke CJ, Middaugh CR,Garsky VM, Johnson RG. 1995. Solution structure of the cytoplasmicdomain of phospholamban: phosphorylation leads to a localperturbation of secondary structure. Biochem 34:7603-7613.

Stokes DL. 1997. Keeping calcium in its place: Ca(2+)-ATPase and phospholamban. Curr Opinion Structural Biol [serial online] 7:550-556. Accessed 2003 Apr 8.

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