This button
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We have already looked at the unliganded
reverse transcriptase complete with all it's functional subdomains and motifs. now begin to look at a functional comparison
of these featured subdomains and motifs in unliganded reverse transcriptase
and reverse transcriptase polymerizing a DNA strand.
Let's first look at the two subdomains, p66
and p51.
As shown on the right, the thumb and fingers grip around the nucleic acid template (in this case, DNA) and hold it in place at the polymerase active site of the palm subdomain
Because reverse transcriptase is a critical
enzyme used by HIV-1, many scientists are trying to target this specific site
References
Doublie S, Sawaya MR, Ellenberger T. 1999.
An open and closed case for all polymerases. Structure (7)2. <http://www.sciencedirect
.com/science?_ob=Article
URL&_aset=B-WA-A-W-AB-MsSAYWA-UUA-AAAAUVBBVD-AAUYZWVAVD-EWAVDBCAC-AB-U&_rdoc=4&_fmt=full&
_udi=B6VSR-4CXCWVD-T&_coverDate=02%2F15
%2F1999&_cdi=6269&_
orig=search&_st=13&_sort
=d&view=c&_acct=C000
058476&_
version=1&_urlVersion=
0&_userid=2665120&md
5=260675e1d2914c88e
9789eb57c0e2e4f>. Accessed 2005 Feb 12.
Goldman M, Marcey D. 2001. HIV-1 Reverse
Transcriptase. The Online Molecular Museum. <http://www.clunet.edu/Bio
Dev/omm/hivrt/hivrt.htm>. Accessed 2005 Feb 12.
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