This web page was produced as an assignment for an undergraduate course at Davidson College
Spike Orthologs:
Murine Hepatitis Virus Spike Protein (MHV)
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Spike Protein Structure and Function
More Information on SARS and the Spike Protein:
Centers for Disease Control and Prevention
Other Interesting Viral Proteins:
Webpage created by Kevin Saunders, an undergraduate student at Davidson College.
The murine hepatitis virus (MHV) spike glycoprotein and the SARS-CoV Spike protein are both type I viral fusion proteins ( Schickli et al., 2004; Rota et al., 2003). The MHV Spike protein is cleaved into two subunits by trypsin and serine proteases ( Schickli et al., 2004). MHV spike subunits function similarly to the SARS-CoV Spike protein subunits ( Schickli et al., 2004). |
| MHV and SARS-CoV spike proteins have conserved amino acid sequences within the amino acid sequence that comprises the fusion core of the Spike protein. |
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| Figure 1. A comparison of amino acids that comprise the fusion core of the Spike Proteins from SARS-CoV and MHV. The red and pink letters are positive matches. The Purple letters are negative matches. Data from NCBI. |
| This ortholog of the S protein is found in the avian infectious bronchitis coronavirus. This spike protein is 1361 amino acids long. There 19 subsequences of amino acids within the avian coronavirus S protein that show sequence similarity to the SARS-CoV S protein. These 19 sub sequences add up to 1119 amino acids. 512 of these 1119 amino acids are positive matches for the SARS-CoV S protein amino acid sequence. |
The Amino Acid Sequence of the Murine Hepatitis Spike Protein |
1 MLFVFILLLP SCLGYIGDFR CINLVNTDTS NASAPIVSTE VVDVSKGIGT YYVLDRVYLN 61 AILLLTGYYP VDGSNYRNLA LTGTNTISLN WYKPPFLSEF NDGIFAKVKN LKASLPKDSI 121 SYFPTIVIGS NFVNTSYTVV LEPYNGIIMA SICHYTICQL PYTDCKPKTG GNKLIGFWHI 181 DLKPPVCILK RNFTFNVNTD WLYFHFYQQG GTFYAYYADV GSATTFLFSS YIGDVLTQYF 241 VLPYVCNTPT AGVFSPQYWV TPLVKRQYLF NFNQKGVITS AVDCASSYTS EIKCKTQSMN 301 PSTGVYDLTG YTVQPVGVVY RRVRNLPDCK IEDWLTAKTV PSPLNWERKT FQNCNFNLSS 361 LLRFVQAESL SCSNIDASKV YGMCFGSVSI DKFAIPNRRR VDLQLGNSGF LQSFNYKIDT 421 RATSCQLYYS LAQNNVTVIN HNPSSWNRRY GFNDVATFGN GKHDVAYAEA CYTVGASYCP 481 CANPSIVSPC TQDKPKAANC PVGTRNRECN PLALGSNLFK CDCTCNPSPL TTYDLRCLQA 541 RSMLGVGDHC EGLGVLEDKC GGSNTCNCSA DAFVGWAKDS CLSNGYCHIF SNFVLNGINS 601 GTTCSTDLQL PNTEVIIGVC VKYDLYGITG QGVFKEVKAD YYNSWQNLLY DVNGNLNGFR 661 DIVTNKTYLI RSCYSGRVSA AYHQDAPEPA LLYRNLKCDY VFNNNISREE TPLNYFDSYL 721 GCVVNADNST EQSVDACDLR MGSGLCVNFS LAHRARRSVS TGYKLTTFEP FTVSIVNDSA 781 ESVGGLYEMQ IPTNFTIASH QEFIQTRSPK VTIDCAAFVC GDYTACRQQL IEYGSFCDNI 841 NAILGEVNNL IDTMQLQVAS ALIQGVTLSS RLADGISGQI DDINFSPLLG CLGSQCSEGT 901 MAAQGRSTIE DLLFDKVKLS DVGFVEAYNN CTGGQEVRDL LCVQSFNGIK VLPPVLSENQ 961 ISGYTAGATA SSMFPPWSAA AGVPFSLSVQ YRINGLGVTM NVLSENQKMI ASAFNNAIGA 1021 IQEGFDATNS ALAKIQSVVN ANAEALNNLL NQLSNRFGAI SASLQEILSR LDALEAQAQI 1081 DRLINGRLTA LNAYVSKQLS DMTLIKVSAA QAVEKVNECV KSQSPRINFC GNGNHILSLV 1141 QNAPYGLYFI HFSYVPTSFT TANVSTGLCI SGDRGLAPKA GYFVQDNGEW KFTGSNYYYP 1201 EPINDKNSVV MSSCAVNYTK APEVFLNTSL PNLPDFKEEL DKWFKNQTSV APDLSLDFEK 1261 LNVTFLDLSG EMNRIQEAIK KLNESYINLK EVGTYEMYVK WPWYVWLLIG LAGVAVCVLL 1321 FFICCCTGCG SCCFKRCGNC CDEYGGHQDS IVIHNILSHE D |
| Sequence taken from Yamada and Yabe, Unpublished . |
Orthologs
Canine coronavirus Spike Protein
Murine Hepatitis Virus Spike Protein
Avian infectious bronchitis coronavirus Spike Protein
Works Cited
Rota, P. et al. 2003. Characterisation of a novel Coronavirus associated with Severe Acute Respiratory Syndrome. Science 300: 1394-1399.
Schickli, J., Thackray, L., Sawicki, S., and Holmes, K. 2004. The N-terminal region of the murine coronavirus spike glycoprotein is associated with the extended host range of viruses from persistently infected murine cells. J Virol. 78(17): 9073-83.