Prothrombin and Trypsin Alignment
wrferv CLUSTAL 2.0.12 multiple sequence alignment
Prothrombin_Human MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANT-FLE 49
prothrombin_Chimpanzee MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANT-FLE 49
prothrombin_Bull MARVRGPRLPGCLALAALFSLVHSQHVFLAHQQASSLLQRARRANKGFLE 50
prothrombinI_Mouse MSHVRGLGLPGCLALAALVSLVHSQHVFLAPQQALSLLQRVRRANSGFLE 50
prothrombin_Rat MLHVRGLGLPGCLALAALASLVHSQHVFLAPQQALSLLQRVRRANSGFLE 50
prothrombin_Chicken MAHSKTTMLQGLLLFGLLHLTLSHDGVFLEKGQALSLLKRPRRANKGFLE 50
prothrombin_Zebrafish -MGAKLAPLLLFLLFGQVFHLTLCHNVFINNKEASQIIR-AKRANT-VFE 47
prothrombin_Lamprey ----MIVAVVMATVAMAMIHATQGAQVFLDRPVASRVLQPRTRRANSFLE 46
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human EVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRD- 98
prothrombin_Chimpanzee EVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRD- 98
prothrombin_Bull EVRKGNLERECLEEPCSREEAFEALESLSATDAFWAKYTACESARNPRE- 99
prothrombinI_Mouse ELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRE- 99
prothrombin_Rat ELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRE- 99
prothrombin_Chicken EMIKGNLERECLEETCNYEEAFEALESTVDTDAFWAKYQVCQGTKMPRT- 99
prothrombin_Zebrafish ELKPGNLERECVEEICDHEEAREVFERVDKTEIFWAKYLGCEGTTLSRTP 97
prothrombin_Lamprey ERLQGNLERECLEETCSHEEAREVYENDAGTAAFWNQYKGCNVDNIRSH- 95
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human ------KLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEI 142
prothrombin_Chimpanzee ------KFAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEI 142
prothrombin_Bull ------KLNECLEGNCAEGVGMNYRGNVSVTRSGIECQLWRSRYPHKPEI 143
prothrombinI_Mouse ------TFMDCLEGRCAMDLGVNYLGTVNVTHTGIQCQLWRSRYPHKPEI 143
prothrombin_Rat ------TFMDCLEGRCAMDLGLNYHGNVSVTHTGIECQLWRSRYPHRPDI 143
prothrombin_Chicken ------TLDACLEGNCAANLGQNYRGTINYTKSGIECQVWTSKYPHIPKF 143
prothrombin_Zebrafish QNINSLRICATTEGDCFINIGAKYAGKVSVTKSGKACQYWKSNFPHK--I 145
prothrombin_Lamprey ----MELFRRCLKGLCITGNGEQYIGNVSITKSGRQCQLWTSNFPHQVRF 141
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human NSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQD-QV 191
prothrombin_Chimpanzee NSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQD-QV 191
prothrombin_Bull NSTTHPGADLRENFCRNPDGSITGPWCYTTSPTLRREECSVPVCGQD-RV 192
prothrombinI_Mouse NSTTHPGADLKENFCRNPDSSTTGPWCYTTDPTVRREECSVPVCGQEGRT 193
prothrombin_Rat NSTTHPGADLKENFCRNPDSSTSGPWCYTTDPTVRREECSIPVCGQEGRT 193
prothrombin_Chicken NASIYP--DLTENYCRNPDNNSEGPWCYTRDPTVEREECPIPVCGQE-RT 190
prothrombin_Zebrafish DEFNVTQLKLQENFCRNPDKHKDGPWCFTRDPTVRRETCNVPKCGEA--- 192
prothrombin_Lamprey KPVDHPEKQLVENFCRNPDASQDGPWCYTRDPLVPREPCSVPSCDEDPSR 191
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human TVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQ 241
prothrombin_Chimpanzee TVAMTPRSEGSSVNLSPPSEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQ 241
prothrombin_Bull TVEVIPRSGGSTTSQSPLLETCVPDRGREYRGRLAVTTHGSRCLAWSSEQ 242
prothrombinI_Mouse TVVMTPRSGGSKDNLSPPLGQCLTERGRLYQGNLAVTTLGSPCLPWNSLP 243
prothrombin_Rat TVKMTPRSRGSKENLSPPLGECLLERGRLYQGNLAVTTLGSPCLAWDSLP 243
prothrombin_Chicken TVEFTPRVKPSTTG-----QPCESEKGMLYTGTLSVTVSGARCLPWASEK 235
prothrombin_Zebrafish -VVPPPKAP----------------------------------------- 200
prothrombin_Lamprey HVPELPRVPPTAPATRPQ-GPCVPGAGEDYRGPLNVTRLGRRCQAWSAR- 239
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human AKALSKHQDFNSAVQLVENFCR--NPDGDEEGVWCYVAGKPGDFGYCDLN 289
prothrombin_Chimpanzee AKALSKHQDFNSAVQLVENFCR--NPDGDEEGVWCYVAGKPGDFGYCDLN 289
prothrombin_Bull AKALSKHQDFNPAVPLAENFCR--NPDGDEEGAWCYVADQPGDFEYCDLN 290
prothrombinI_Mouse AKTLSKYQDFDPEVKLVENFCR--NPDWDEEGAWCYVAGQPGDFEYCNLN 291
prothrombin_Rat TKTLSKYQNFDPEVKLVQNFCR--NPDRDEEGAWCFVAQQPG-FEYCSLN 290
prothrombin_Chicken AKALLQDKTINPEVKLLENYCR--NPDADDEGVWCVID-EPPYFEYCDLH 282
prothrombin_Zebrafish ----------------LDKFVE--EGGGRE-------------------- 212
prothrombin_Lamprey -------------TPHVSRFSPSTHSAAGLEGAWCYTTDPGLAVDYCALH 276
trypsin_Zebrafish --------------------------------------------------
chymotrypsin_human --------------------------------------------------
chymotrypsin_Chimpanzee --------------------------------------------------
chymotrypsin_Mouse --------------------------------------------------
chymotrypsin_Rat --------------------------------------------------
chymotrypsin_Bull --------------------------------------------------
chymotrypsin_Zebrafish --------------------------------------------------
Prothrombin_Human YCEEAVEEETGDGLDEDSD--RAIEGRTATSEYQTFFNPRTFGSGEADCG 337
prothrombin_Chimpanzee YCEEAVEEETGDGLDEDPD--RAIEGRTATSEYQPFFNPRTFGSGEADCG 337
prothrombin_Bull YCEEPVDGDLGDRLGEDPDPDAAIEGRTSEDHFQPFFNEKTFGAGEADCG 340
prothrombinI_Mouse YCEEAVGEEN-----YDVD--ESIAGRTTDAEFHTFFNEKTFGLGEADCG 334
prothrombin_Rat YCDEAVGEEN-----HDGD--ESIAGRTTDAEFHTFFDERTFGLGEADCG 333
prothrombin_Chicken YCDSSLEDEN--------EQVEEIAGRTIFQEFKTFFDEKTFGEGEADCG 324
prothrombin_Zebrafish --------------------------RTTLDQRKAFFNPRSFGNGELDCG 236
prothrombin_Lamprey YCDEDDIFMEAP----KEEGGRGRTTATGTDPKMTLINPRSFGSGQLECG 322
trypsin_Zebrafish ---------------------------------------------MKAFI 5
chymotrypsin_human ------------------------------------------------CG 2
chymotrypsin_Chimpanzee --------------------------------MLGITVLAALLACASSCG 18
chymotrypsin_Mouse ------------------------------MAFLWLVSCFALVGATFGCG 20
chymotrypsin_Rat ------------------------------MAFLWLVSCFALVGATFGCG 20
chymotrypsin_Bull ------------------------------MAPLWLLSCFALVGAAFGCG 20
chymotrypsin_Zebrafish ------------------------------MAFLWILSCLAFFGAAYGCG 20
Prothrombin_Human LRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFR--KS 385
prothrombin_Chimpanzee LRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFR--KS 385
prothrombin_Bull LRPLFEKKQVQDQTEKELFESYIEGRIVEGQDAEVGLSPWQVMLFR--KS 388
prothrombinI_Mouse LRPLFEKKSLKDTTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFR--KS 382
prothrombin_Rat LRPLFEKKSLTDKTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFR--KS 381
prothrombin_Chicken TRPLFEKKQITDQSEKELMDSYMGGRVVHGNDAEVGSAPWQVMLYK--KS 372
prothrombin_Zebrafish ERPLFEKINKADKNEKELLMSYTGSRIVGGDEAEVASAPWQVMLYK--RS 284
prothrombin_Lamprey KRPMFELMGKEDVGEEELQESQRQ-RVVHGDNAEEGSAPWQVMLYR--KS 369
trypsin_Zebrafish LLALFAVAYAAPLGDD-------DDKIVGGYECTKNGVPYQVSLNS--G- 45
chymotrypsin_human VPSFPPNLS---------------ARVVGGEDARPHSWPWQISLQYLKDD 37
chymotrypsin_Chimpanzee VPSFSPNLS---------------ARVVGGEDARPHSWPWQISLQYLKND 53
chymotrypsin_Mouse VPAIQPVLTGL-------------SRIVNGEDAIPGSWPWQVSLQ---DR 54
chymotrypsin_Rat VPTIQPVLTGL-------------SRIVNGEDAIPGSWPWQVSLQ---DK 54
chymotrypsin_Bull VPAIQPVLSGL-------------ARIVNGEDAVPGSWPWQVSLQ---DS 54
chymotrypsin_Zebrafish IPAIPPVVTGY-------------ARIVNGEEARPHSWPWQVSLQ---DS 54
.: ::* * :. *:*: *
Prothrombin_Human PQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYE 435
prothrombin_Chimpanzee PQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYE 435
prothrombin_Bull PQELLCGASLISDRWVLTAAHCLLYPPWDKNFTVDDLLVRIGKHSRTRYE 438
prothrombinI_Mouse PQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYE 432
prothrombin_Rat PQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYE 431
prothrombin_Chicken PQELLCGASLISNSWILTAAHCLLYPPWDKNLTTNDILVRMGLHFRAKYE 422
prothrombin_Zebrafish PQELLCGASLISDEWILTAAHCILYPPWNKNFTINDIIVRLGKHSRTKYE 334
prothrombin_Lamprey PMDMLCGASLISDQWVLTAAHCVFYPPWDKDFTAKELAVRIGKHNKAGYE 419
trypsin_Zebrafish --YHFCGGSLISNLWVVSAAHCYKSR----------VQVRLGEHN-IDVT 82
chymotrypsin_human TWRHTCGGTLIASNFVLTAAHCISNTW--------TYRVAVGKNNLEVED 79
chymotrypsin_Chimpanzee TWRHTCGGTLIASNFVLTAAHCISNTR--------TYRVAVGKNNLEVED 95
chymotrypsin_Mouse TGFHFCGGSLISENWVVTAAHCGVKT---------TDVVVAGEFDQGS-D 94
chymotrypsin_Rat TGFHFCGGSLISEDWVVTAAHCGVKT---------SDVVVAGEFDQGS-D 94
chymotrypsin_Bull TGFHFCGGSLISEDWVVTAAHCGVTT---------SDVVVAGEFDQGL-Q 94
chymotrypsin_Zebrafish TGFHFCGGSLINENWVVTAAHCNVRT---------SHRVILGEHDRSS-N 94
**.:** . ::::**** * *
Prothrombin_Human RNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPD 485
prothrombin_Chimpanzee RNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPD 485
prothrombin_Bull RKVEKISMLDKIYIHPRYNWKENLDRDIALLKLKRPIELSDYIHPVCLPD 488
prothrombinI_Mouse RNVEKISMLEKIYVHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPD 482
prothrombin_Rat RNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPD 481
prothrombin_Chicken RNKEKIVLLDKVIIHPKYNWKENMDRDIALLHLKRPVIFSDYIHPVCLPT 472
prothrombin_Zebrafish RGIEKIVAIDEIIVHPKYNWKENLNRDIALLHMKKPVVFTSEIHPVCLPT 384
prothrombin_Lamprey KDREKISNVDKIIIHSKYNWKENMERDIALLHLEKPITFTQYIVPICLPT 469
trypsin_Zebrafish EGTEQFINSEKVIRHPSYNSNT-LDNDVMLIKLSSSAQINSYVKTVSLPS 131
chymotrypsin_human EEGSLFVGVDTIHVHKRWNALL-LRNDIALIKLAEHVELSDTIQVACLPE 128
chymotrypsin_Chimpanzee EEGSLFVGVDTIHVHKRWNALL-LRNDIALIKLAEHVELSDTIQVACLPE 144
chymotrypsin_Mouse EENVQVLKIAQVFKNPKFNSFT-VRNDITLLKLATPAQFSETVSAVCLPT 143
chymotrypsin_Rat EENIQVLKIAQVFKNPKFNMFT-VRNDITLLKLATPAQFSETVSAVCLPN 143
chymotrypsin_Bull TEDTQVLKIGKVFKNPKFSILT-VRNDITLLKLATPAQFSETVSAVCLPS 143
chymotrypsin_Zebrafish AEAIQTIAVGKSIKHPNYNSFT-INNDILLIKLATPAKINTHVSPVCLAE 143
: :. : .*: *::: :. : .*.
Prothrombin_Human RETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPV 535
prothrombin_Chimpanzee RETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPV 535
prothrombin_Bull KQTAAKLLHAGFKGRVTGWGNRRETWTTSVAEVQPSVLQVVNLPLVERPV 538
prothrombinI_Mouse KQTVTSLLRAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPV 532
prothrombin_Rat KQTVTSLLQAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPV 531
prothrombin_Chicken KELVQRLMLAGFKGRVTGWGNLKETWATTP-ENLPTVLQQLNLPIVDQNT 521
prothrombin_Zebrafish KSIAKNLMFAGYKGRVTGWGNLRESWTSNP-SNLPAVLQQIHLPIVDQSI 433
prothrombin_Lamprey RDVAVNLLKSGFKGRVTGWGNLFQTWTNNP-RAQPKVLQMINLPIVDPSR 518
trypsin_Zebrafish -----SCASSGTSCLISGWGN-----MSASGSNYPSRLMCLNAPILSDST 171
chymotrypsin_human KD---SLLPKDYPCYVTGWGR-----LWTNGP-IADKLQQGLQPVVDHAT 169
chymotrypsin_Chimpanzee KD---SLLPKDYPCYVTGWGR-----LWTNGP-IADELQQGLQPVVDHAT 185
chymotrypsin_Mouse VD---DDFPAGTLCATTGWGK-----TKYNALKTPDKLQQAALPIVSEAK 185
chymotrypsin_Rat VD---DDFPPGTVCATTGWGK-----TKYNALKTPEKLQQAALPIVSEAD 185
chymotrypsin_Bull AD---EDFPAGMLCATTGWGK-----TKYNALKTPDKLQQATLPIVSNTD 185
chymotrypsin_Zebrafish TN---DNFPGGMKCVTSGWGL-----TRYNAPDTPALLQQAALPLLTNDD 185
. :*** . * *::
Prothrombin_Human CK--DSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWY 583
prothrombin_Chimpanzee CK--DSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWY 583
prothrombin_Bull CK--ASTRIRITDNMFCAGYKPGEGKRGDACEGDSGGPFVMKSPYNNRWY 586
prothrombinI_Mouse CK--ASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSPFNNRWY 580
prothrombin_Rat CK--ASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSPYNHRWY 579
prothrombin_Chicken CK--ASTRVKVTDNMFCAGYSPEDSKRGDACEGDSGGPFVMKNPDDNRWY 569
prothrombin_Zebrafish CR--NSTSVIITDNMFCAGYQPDDSKRGDACEGDSGGPFVMKSPSDNRWY 481
prothrombin_Lamprey CQ--ESTTHRITANMLCAGYEPEDVKRGDACEGDSGGPFIMKDFENKRWY 566
trypsin_Zebrafish CR--NAYPGQISSNMFCAGFMEGGK---DSCQGDSGGPVVCNN------Q 210
chymotrypsin_human CSRIDWWGFRVKKTMVCAG----GDGVISACNGDSGGPLNCQLE-NGSWE 214
chymotrypsin_Chimpanzee CSRIDWWGFRVKKTMVCAG----GDGVISACNGDSGGPLNCQLE-NGSWE 230
chymotrypsin_Mouse CK--ESWGSKITDVMICAG----ASG-VSSCMGDSGGPLVCQK--DGVWT 226
chymotrypsin_Rat CK--KSWGSKITDVMTCAG----ASG-VSSCMGDSGGPLVCQK--DGVWT 226
chymotrypsin_Bull CR--KYWGSRVTDVMICAG----ASG-VSSCMGDSGGPLVCQK--NGAWT 226
chymotrypsin_Zebrafish CK--RYWGTNITDLMICAG----ASG-VSSCMGDSGGPLVCEN--NRVWT 226
* :. * *** .:* ******. :
Prothrombin_Human QMGIVSWGEG--CDRDGKYGFYTHVFRLKKWIQKVIDQFGE------- 622
prothrombin_Chimpanzee QMGIVSWGEG--CDRDGKYGFYTHVFRLKKWIQKVIDQFGE------- 622
prothrombin_Bull QMGIVSWGEG--CDRDGKYGFYTHVFRLKKWIQKVIDRLGS------- 625
prothrombinI_Mouse QMGIVSWGEG--CDRKGKYGFYTHVFRLKRWIQKVIDQFG-------- 618
prothrombin_Rat QMGIVSWGEG--CDRNGKYGFYTHVFRLKRWMQKVIDQHR-------- 617
prothrombin_Chicken QVGIVSWGEG--CDRDGKYGFYTHVFRLKKWMRKTIEKQG-------- 607
prothrombin_Zebrafish QIGIVSWGEG--CDRDGKYGFYTHLFRMRRWMKKVIEKTDSGDDE--- 524
prothrombin_Lamprey QMGIVSWGEG--CDRDGKYGIYTHVYRLRKWINKVIGEPDTTRYPSIN 612
trypsin_Zebrafish LQGIVSWGYG--CAQRNKPGVYAKVCTSPPGSETP------------- 243
chymotrypsin_human VFGIVSFGSRRGCNTRKKPVVYTRVSAYIDWINEKMQL---------- 252
chymotrypsin_Chimpanzee VFGIVSFGSQRGCNTPKKPVVYTRVSAYIDWINEKMQL---------- 268
chymotrypsin_Mouse LAGIVSWGSG-FCSTS-TPAVYARVTALMPWVQEILEAN--------- 263
chymotrypsin_Rat LAGIVSWGSG-VCSTS-TPAVYSRVTALMPWVQQILEAN--------- 263
chymotrypsin_Bull LAGIVSWGSS-TCSTS-TPAVYARVTALMPWVQETLAAN--------- 263
chymotrypsin_Zebrafish LVGIVSWGSS-TCSTS-TPAVYARVTKLRAWVDQTIASN--------- 263
****:* * . .*:::
Jmol
Paper Review
Figure 5: ClustalW alignment of trypsin and prothrombin proteins from several different species. Notice the high level of conservation of the protein sequence in the trypsin-like serine protease Domain of the prothrombin proteins (Made using ClustalW)