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Return to "How to Search Genbank"
Again, you will get a huge response. The most useful information in this is the size of the total sequence, the source of the DNA, where the sequence was published, and the sequence itself. An example is shown below:
Database: GenBank (86.0, 12/10/94)
Query: u17997 m62653
Parse status: OK: 1 document retrieved.
Documents selected: 1-1 (up to 1000 lines)
LOCUS AEVGFPA 966 bp ss-mRNA INV 06-APR-1992
DEFINITION Aequorea victoria green-fluorescent protein mRNA, complete cds.
ACCESSION M62653
KEYWORDS aequorin-associated energy-transfer acceptor; bioluminescence;
green-fluorescent protein; photocyte.
SOURCE Aequorea victoria (library: lambda gt10) cDNA to mRNA.
ORGANISM Aequorea victoria
Eukaryota; Animalia; Eumetazoa; ; Cnidaria; Hydrozoa; Hydroida;
Leptomedusae; Aequoreidae.
REFERENCE 1 (bases 1 to 966)
AUTHORS Prasher,D.C., Eckenrode,V.K., Ward,W.W., Prendergast,F.G. and
Cormier,M.J.
TITLE Primary structure of the Aequorea victoria green-fluorescent
protein
JOURNAL Gene 111, 229-233 (1992)
STANDARD full automatic
COMMENT
NCBI gi: 155660
FEATURES Location/Qualifiers
source 1..966
/organism="Aequorea victoria"
/sequenced_mol="cDNA to mRNA"
/tissue_lib="lambda gt10"
CDS 26..742
/gene="GFP"
/note="NCBI gi: 155661"
/codon_start=1
/product="green-fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
VLLEFVTAAGITHGMDELYK"
misc_feature 218..226
/gene="GFP"
/note="fluorescent chromophore"
/evidence=experimental
BASE COUNT 336 a 169 c 175 g 286 t
ORIGIN
1 tacacacgaa taaaagataa caaagatgag taaaggagaa gaacttttca ctggagttgt
61 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
121 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
181 actacctgtt ccatggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc
241 aagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
301 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
361 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
421 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
481 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
541 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
601 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
661 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
721 catggatgaa ctatacaaat aaatgtccag acttccaatt gacactaaag tgtccgaaca
781 attactaaaa tctcagggtt cctggttaaa ttcaggctga gatattattt atatatttat
841 agattcatta aaattgtatg aataatttat tgatgttatt gatagaggtt attttcttat
901 taaacaggct acttggagtg tattcttaat tctatattaa ttacaatttg atttgacttg
961 ctcaaa