One-Time Flippable DNA Segments for Biological Computers
by Bruce Henschen
This semester, I have been working on engineering Hix sites, which enable a protein from Salmonella called Hin recombinase to flip a segment of DNA:
Specifically, we would like to be able to control the Hin/Hix mechanism by allowing DNA segments to flip once then become "frozen" in the correct orientation:
Sites such as these would give us better control over how E. coli flip genes using Hin recombinase.
To Test these sites, I designed an assay that would determine whether pLac could be flipped when it rested between different pairs of Hix sites.
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