Exploring GEO
(Gene Expression Omnibus)


1) Go to the GEO database.

2) Click on the DataSets button and scroll down until you find a data set of interest. For this example, I chose yeast heat shock from 25 to 37 degrees. When you have selected your data, click on the "go to full dataset record" link.


4) Click on the Data analysis link and choose the default hierarchical clustering method (for a starting place at least). You will see a large, colorful dendrogram showing clustered genes over the timecourse of this experiment.

5) Resize the dotted box to highlight a small area of interest to you. Choose a section with highly induced or repressed genes.

6) Click on the "Plot selected gene profiles button to see a graph of their expression profiles. You can narrow this box down a second time if you wish.

7) At the bottom of the line graph, choose the option of getting profiles in Entrez-GEO.

8) Finally, find a gene of interest to see the quantitative values for this gene at the various time points.

Did you find GEO to be an easy to use database? After this tutorial, do you see the benefit of a single site with vast datasets?