1) Go to the DIP search page. Click on the "Login" button in the top right corner. If you have not registered (it's free to academic users), you will need to do so. Enter Sup35 in the "Node ID" blank space and click on "Query DIP". Sup35 is the prion-like protein in yeast.

2) You will get an unimpressive results page. Click on the link under the heading "DIP Node" and then click on the word "graph" in the top right corner. A new window will pop up.

3) What you have found are all the proteins that interact with your query protein (red node). Click on the "big" link to expand the view. Then click on the "LEGEND" link to help you define what all the colors and lines are telling you.

4) What protein has the best data for interacting with Sup35? How many other proteins have experimental evidence to support physical interaction? How many proteins have predicted interactions? How could you test these predictions?

Discovery Questions