LOCUS CHKPSYN 4698 bp mRNA VRT 28-APR-1993
DEFINITION Chicken prostaglandin G/H synthase mRNA, complete cds.
ACCESSION M64990
VERSION M64990.1 GI:212620
KEYWORDS prostaglandin synthase.
SOURCE Chicken Rous sarcoma virus induced fibroblast cell line CEF-147,
cDNA to mRNA.
ORGANISM Gallus gallus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Archosauria;
Aves; Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus.
REFERENCE 1 (bases 1 to 4698)
AUTHORS Xie,W., Chipman,J.G., Robertson,D.L., Erikson,R.L. and Simmons,D.L.
TITLE Expression of a mitogen-responsive gene encoding prostaglandin
synthase is regulated by mRNA splicing
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 88, 2692-2696 (1991)
MEDLINE 91187858
FEATURES Location/Qualifiers
source 1..4698
/organism="Gallus gallus"
/db_xref="taxon:9031"
/cell_type="CEF-147"
/tissue_type="fibroblast"
gene join(48..99,653..2412)
/gene="prostaglandin synthase"
CDS join(48..99,653..2412)
/gene="prostaglandin synthase"
/note="Due to the unspliced intron this mRNA when present
in nonproliferating cells was found to be nonfunctional.
In src transformed cells the protein is found in
relatively high abundance."
/codon_start=1
/product="prostaglandin synthase"
/protein_id="AAA49050.1"
/db_xref="GI:212621"
/translation="MLLPCALLAALLAAGHAANPCCSLPCQNRGVCMTTGFDRYECDC
TRTGYYGENCTTPEFFTWLKLILKPTPNTVHYILTHFKGVWNIINNISFLRDTIMRYV
LTSRSHLIDSPPTYNSDYSYKSWEAYSNLSYYTRSLPPVGHDCPTPMGVKGKKELPDS
KLIVEKFLLRRKFIPDPQGTNVMFTFFAQHFTHQFFKTDHKKGPGFTKAYGHGVDLNH
IYGETLERQLKLRLRKDGKLKYQMIDGEMYPPTVKDTQAEMIYPPHVPEHLQFSVGQE
VFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWDDEQLFQTTRLILIGETIKIVIEDY
VQHLSGYHFKLKFDPELLFNQRFQYQNRIAAEFNTLYHWHPLLPDTFQIHNQEYTFQQ
FLYNNSIMLEHGLSHMVKSFSKQSAGRVAGGKNVPAAVQKVAKASIDQSRQMRYQSLN
EYRKRFMLKPFKSFEELTGEKEMAAELEELYGDIDAMELYPGLLVEKPRPGAIFGETM
VEIGAPFSLKGLMGNTICSPEYWKPSTFGGKVGFEIINTASLQKLICNNVKGCPFTAF
HVLNPEPTEATINVSTSNTAMEDINPTLLLKEQSAEL"
intron 100..652
/gene="prostaglandin synthase"
/note="non-spiced out intron preventing expression in
non-proliferating cells"
polyA_site 4698
/gene="prostaglandin synthase"
BASE COUNT 1312 a 944 c 1058 g 1384 t
ORIGIN
1 tggaatttca gtagttgcga ggaaagcgta aagaagcccg ccgggatatg ctgctgcctt
61 gtgcactgct ggccgctctc cttgctgccg gccacgcagg taagcgtggg gcgcgctggg
121 ggctccggct ctgcggggct ccggctctgc ggggctccgg ctctgcgggg ctccggctct
181 gcggggctcc ggctctgcgg ggctccggct ctgcggggct ccggctctgc ggggctccgg
241 ctctgcgggg ctccggctct gcggggctcc ggctctgcgg ggctccggct ctgcggggct
301 ccggctctgc ggggctccgg ctctgcgggg ctccggctct gcggggctcc ggctctgcgg
361 ggctccggct ctgcggggct ccggctctgc ggggctccgg ctctgcgtcc cggtgctgcg
421 cggctgcggc gcggggggcc cgcggcgatg ctcacagccg gggagtaggt gttctgtttt
481 acgtacctcg tgactccgaa gtcagtgcag caggagagct gtcagatccc ctctgcgttc
541 ggagctggtt cgttgcagcc cgcaaacagc gcgcttccca gttcgtgtca ctgatattgc
601 cgggagctgc ccgaagtctc gtgcaagtgg aactcgttct gctgcctgca ctccaaccct
661 tgctgctccc tcccatgtca gaacagaggt gtatgcatga caacaggatt tgatcggtat
721 gaatgtgact gcacgaggac gggctattat ggggaaaact gtacaacacc ggaattcttc
781 acgtggctga aactaatatt gaaacctaca ccaaatactg tccactacat tctcacccac
841 ttcaaaggag tctggaacat catcaacaac atttccttct tacgagatac tattatgaga
901 tacgtgttga cgtcgagatc acacttgatt gacagcccac caacatacaa tagtgactac
961 agttacaaat cctgggaagc ttattccaat ctttcctatt acacaagaag ccttccaccg
1021 gtaggacatg actgtccaac accaatgggt gttaaaggta agaaagagct cccagattca
1081 aagctgattg tggagaaatt cttgctgcgg agaaaattta ttcctgaccc acaaggcaca
1141 aatgtgatgt tcacattctt tgcccagcac ttcactcatc aattctttaa gacagaccat
1201 aagaaagggc ctggcttcac taaagcttac ggccatgggg ttgacttgaa ccacatttat
1261 ggtgagactc tggagaggca acttaaattg agacttcgca aggatggaaa actaaagtac
1321 cagatgattg atggagaaat gtatccacca acagtgaagg acactcaagc agagatgatc
1381 taccctcctc atgttcctga gcatctgcag ttttctgttg ggcaggaggt gtttggcttg
1441 gtcccaggcc taatgatgta tgcaacgata tggctgagag aacacaatag agtctgtgac
1501 gtcttgaaac aggagcatcc agagtgggat gatgagcagc tatttcagac tacaagactc
1561 atattgatag gagaaacgat caagatagtt attgaagatt atgtgcagca cttaagtggc
1621 taccatttca aattgaagtt tgaccctgag cttctgttca accagcgttt tcaataccag
1681 aaccgaatcg cagctgaatt caatactctg taccactggc acccccttct gcctgacact
1741 tttcagatac ataaccagga gtacacattc cagcagttcc tctacaacaa ctccataatg
1801 ctggaacatg gcctttccca tatggtgaaa tctttttcca agcaaagtgc tggtagggtt
1861 gctggtggga aaaatgttcc agctgcagta cagaaagtag caaaggcttc aattgaccaa
1921 agcagacaaa tgagatacca gtctttgaat gagtacagga aacgcttcat gttgaaacca
1981 ttcaaatcat ttgaagaact tacaggagaa aaagaaatgg ctgctgaact agaagagctt
2041 tatggagaca tagatgctat ggaactgtat ccaggccttc ttgtagaaaa gccaagacca
2101 ggtgccatct ttggtgaaac aatggtagaa attggcgcac cgttctccct gaaaggactg
2161 atgggaaata caatatgctc ccctgagtac tggaagccaa gcacttttgg tggaaaagtg
2221 gggtttgaaa taatcaatac tgcctcctta cagaagctca tctgcaacaa tgtgaaaggc
2281 tgtcctttca ctgctttcca tgtcttaaat cctgaaccca cagaggcaac tattaatgtt
2341 agtacctcaa acacagcaat ggaagatatc aaccccacac tactactgaa agagcaatct
2401 gctgagttgt aaattagcca cctaatttat ttatttaagt tattctattt atagctctag
2461 tatttagaaa acaaaaagca accttttaca tcttcacttt gagggttgcc ctagattcct
2521 tactcaggta aggcttcatt ttagaaaaag ggattttctg tgttttagct ctgcaatagt
2581 gaatcccttg tttaaagcct tctgtaacag aactacgtat tccaaaatgg actaagttcc
2641 agttttttac ttgtttgtaa cttgacaaag ggtgttaggg ttttttgtct tcttgctttg
2701 ataggtcaca taacactaca aaaagcaagt tattgcctca gcaatttttt aatttgaagg
2761 tctgttctca agtttaaaaa tgagaacttg atttaatgaa aactgccaga aaagattctg
2821 aattgtgtga gcactcgaag attaacaaat acatgtttga gacttgcttg tttaaagtca
2881 gaaaatgcat tgcatttatc aatgatacta cctctccctc atgcaaataa ggcactttgt
2941 acagtaattt acgtgatttt ttttgtatgt ttgtcctttt gtgtggcatt atattttttc
3001 atcattttta atttaattat taatttaatt atttaaggat aatagaactt gtctgagatg
3061 ctacctgttt ttgaagcatg tgtcacagaa gtattattta agtgagatga tgttaacagt
3121 ggtactgagc ttagacatga ctgtcaggca ggaaaacaag gctgcttgta tatcagtaga
3181 cattttattt tgtgactctg ggaagatctt aaacttaatc caaagtaata gtgtgggttt
3241 ttttcttgtt ttatttttgt agctgtaatt ctctggttag tataaattct ctggacaata
3301 taaatctgtc actctaaata aaactataca ttctttttgt ccttaagggc atgactgtaa
3361 tataggttta tgtagccaaa ctgaaattat tggggaataa tttagaaaat taaaaaaata
3421 aataaaaaat tgggatgctg tataaacata accagcactg actgagctaa acttcaaggc
3481 accaaggctt tgttagtgta ggtctatata aatagagctc taactcatga ctatgaattg
3541 tttatttggt tggagggggg atgttaatgt tttatttatt aaagtataaa cagaggcatt
3601 ttaaaggagt ggcttaagct ctaaggagaa aaaaaataat agctagcaaa gcacaaaata
3661 tcccaacaca tttgtcctgg tccagcgcta aagtatctag tatcattggg gcacataaga
3721 acttaagtgg gatcttgtac cagaatttca acctttgcat ataagggtat ctttttttaa
3781 tttctagaga ggaaatgtag aagatgatct tcccataaac tcagctgggg aactgtagat
3841 aaaaagaata ttacttcaga ctggagtaga gcagcaacct ggtattctgt gccccccagc
3901 caaaattcct tggctacttg tggaaaagga cttgttttat atttccattt gaatctcttg
3961 aatgcttgta gaggcttgtg ctgtaatggc tgtggtacta gaatactttg acttctgtat
4021 ttctattgat ttgtttaaag atgctgatta tcttctacag tgcaaacttg taactggtag
4081 catgaatagc tgactccaga tctcctacag caattgatac cagtgactga tttgaggtgg
4141 tccttattat aaatgctttg ggagatggaa gggtgtttgt ttgtttttta tttgaggcac
4201 aaagaaggaa ggaaattcag taactttgta caaaaaaggg gccagtactg tgttcaatta
4261 atgaaactgc ttaattattg gttaagtcat gtaactgtca ttcagccacc tttggaacac
4321 gctttagtgc acctacctac catttgcaag gaaaactgta tgacttaaaa tcaaaggaag
4381 acaagtctgg gcagatctag ttattttgag gtaggtaaga aagaattcat gaaaataagt
4441 tcatttcagc caagttctta caagctgctt tggacaggaa tccagttcac tctacacttt
4501 gtactctgaa gccatgtttt aacacttatc tgtgacccaa cctgctgggt ttggctgggt
4561 tcaagttgct agtgataaat gtgtatgttt gagattattt atttaaaatt ctgtgaattg
4621 tttttcactc tatttttata ggacaagaat gtttttttta aaatgtgatt gtttatataa
4681 ataaatcgaa aacactgt