AJ009973 . Aspergillus niger ...[gi:4140254]
                                                                                         PubMed, Protein
 

                       LOCUS       ANI9973      2463 bp    DNA             PLN       08-JAN-1999
                       DEFINITION  Aspergillus niger hxk gene.
                       ACCESSION   AJ009973
                       VERSION     AJ009973.1  GI:4140254
                       KEYWORDS    hexokinase; hxk gene.
                       SOURCE      Aspergillus niger.
                         ORGANISM  Aspergillus niger
                                   Eukaryota; Fungi; Ascomycota; Euascomycetes; Plectomycetes;
                                   Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
                       REFERENCE   1  (bases 1 to 2463)
                         AUTHORS   Panneman,H.
                         TITLE     Direct Submission
                         JOURNAL   Submitted (31-JUL-1998) Panneman H., Molecular Genetics of
                                   Industrial Microorganisms, Wageningen Agricultural University,
                                   Dreijenlaan 2, 6703 HA, THE NETHERLANDS
                       REFERENCE   2  (bases 1 to 2463)
                         AUTHORS   Panneman,H., Ruijter,G.J., van den Broeck,H.C. and Visser,J.
                         TITLE     Cloning and biochemical characterisation of Aspergillus niger
                                   hexokinase--the enzyme is strongly inhibited by physiological
                                   concentrations of trehalose 6-phosphate
                         JOURNAL   Eur. J. Biochem. 258 (1), 223-232 (1998)
                         MEDLINE   99067038
                       FEATURES             Location/Qualifiers
                            source          1..2463
                                            /organism="Aspergillus niger"
                                            /strain="N400"
                                            /db_xref="taxon:5061"
                                            /chromosome="6"
                            mRNA            join(317..356,447..569,652..677,767..1174,1239..2114)
                                            /gene="hxk"
                                            /product="hexokinase"
                                            /evidence=experimental
                            gene            317..2114
                                            /gene="hxk"
                            exon            317..356
                                            /gene="hxk"
                                            /number=1
                            CDS             join(317..356,447..569,652..677,767..1174,1239..2114)
                                            /gene="hxk"
                                            /EC_number="2.7.1.1"
                                            /function="phosphorylation of glucose and fructose"
                                            /codon_start=1
                                            /evidence=experimental
                                            /product="hexokinase"
                                            /protein_id="CAA08922.1"
                                            /db_xref="GI:4140255"
                                            /translation="MVGIGPKRPPSRKGSMADVPQNLLQQIKDFEDQFTVDRSKLKQI
                                            VNHFVKELEKGLSVEGGNIPMNVTWVLGFPDGDEQGTFLALDMGGTNLRVCEITLTQE
                                            KGAFDITQSKYRMPEELKTGTAEELWEYIADCLQQFIESHHENEKISKLPLGFTFSYP
                                            ATQDYIDHGVLQRWTKGFDIDGVEGHDVVPPLEAILQKRGLPIKVAALINDTTGTLIA
                                            SSYTDSDMKIGCIFGTGVNAAYMENAGSIPKLAHMNLPADMPVAINCEYGAFDNEHIV
                                            LPLTKYDHIIDRDSPRPGQQAFEKMTAGLYLGEIFRLALMDLVENRPGLIFNGQDTTK
                                            LRKPYILDASFLAAIEEDPYENLEETEELMERELNIKATPAELEMIRRLAELIGTRAA
                                            RLSACGVAAICTKKKIDSCHVGADGSVFTKYPHFKARGAKALREILDWAPEEQDKVTI
                                            MAAEDGSGVGAALIAALTLKRVKAGNLAGIRNMADMKTLL"
                            intron          357..446
                                            /gene="hxk"
                                            /number=1
                            exon            447..569
                                            /gene="hxk"
                                            /number=2
                            intron          570..651
                                            /gene="hxk"
                                            /number=2
                            exon            652..677
                                            /gene="hxk"
                                            /number=3
                            intron          678..766
                                            /gene="hxk"
                                            /number=3
                            exon            767..1174
                                            /gene="hxk"
                                            /number=4
                            intron          1175..1238
                                            /gene="hxk"
                                            /number=4
                            exon            1239..2114
                                            /gene="hxk"
                                            /number=5
                       BASE COUNT      532 a    767 c    601 g    563 t
                       ORIGIN
                               1 ctcgagatac tttgatgcaa gaggacaaga catgaagcgg cgagacaccc aaagcaacaa
                              61 ccggtgaagg cgatccctcc cctccactgc ggggcccaac tttagttcaa ctattattat
                             121 tattattatt gtttgcttcc acgcttatga ttctgctgct gccccacgtc actccccctc
                             181 gatcttcttt catcccggtt tctaattcta actctctctg ctccatcttc tccttgactt
                             241 ctctacattc ttctttttga tcaaactatc cttctctact acctctttct ccatctaata
                             301 tctattcacc gtcatcatgg ttggaatcgg tcctaagcgt cccccctccc gcaagggtaa
                             361 actcatagct ctcctcctcc cacttccccc ctccatttct cgggatgccg atcgctgacc
                             421 cgatggcttc cctcgtgctt ctccaggttc catggccgat gttccccaga acctcttgca
                             481 gcagatcaag gacttcgagg accaattcac cgtcgatcgc tccaagctca agcagattgt
                             541 caaccacttt gtcaaggaat tggaaaaggg tgcgtcttcc acactttacc cgcttccaat
                             601 gcggccccgg aatgcatcac tccgactgac tctctttctt ccatgcaata ggtctctctg
                             661 tcgagggtgg aaacatcgta agtgctttgg ctctcgcaat gcggcattcg actcaattcg
                             721 tttttgtttc ctccgttctc gactaacgct tctcttcccc tctcagccta tgaacgtcac
                             781 ctgggttctg ggattccccg atggcgacga acagggtact ttcctcgccc tcgacatggg
                             841 tggcaccaac ctgcgtgttt gtgagatcac cctgacccag gagaagggtg ccttcgacat
                             901 cacccagtcc aagtaccgca tgcccgagga attgaagacc ggtaccgccg aggagctgtg
                             961 ggaatacatc gccgactgcc tgcagcaatt catcgagtcc caccacgaga acgagaagat
                            1021 ctccaagctg cccctgggtt tcaccttctc ctaccccgcc acccaggatt acatcgacca
                            1081 cggtgtcctg cagcgctgga ccaagggttt cgacattgat ggtgtcgagg gccacgacgt
                            1141 cgtcccgccg ttggaggcca tcctgcagaa gcgcgtacgt cgaattcccc tccaccccca
                            1201 tgatgtgtaa cccgagctaa tctggtatgt ctctctaggg cctgcccatc aaggtggctg
                            1261 cactgatcaa cgacaccacc ggaaccctca tcgcctcttc ttacaccgac tccgacatga
                            1321 agatcggctg catcttcggt accggtgtca acgccgccta catggagaac gccggctcca
                            1381 tccccaagct ggctcacatg aacctgcccg ccgacatgcc cgtggctatc aactgcgagt
                            1441 acggtgcttt cgacaacgag cacatcgtgc tgcctctgac caagtacgac cacatcatcg
                            1501 accgcgactc gccccgtccc ggtcagcagg ccttcgagaa gatgaccgcc ggtctgtacc
                            1561 tgggtgagat cttccgtctg gccctgatgg acctggtgga gaaccgcccc ggcctcatct
                            1621 tcaacggcca ggacaccacc aagctgcgca agccctacat cctggatgcc tccttcctgg
                            1681 cagccatcga ggaggacccc tacgagaacc tggaggagac cgaggagctc atggagcgcg
                            1741 agctcaacat caaggccacc ccggcggagc tggagatgat ccgccgcctg gccgagctga
                            1801 tcggtacgcg tgccgctcgc ctgtcggcct gcggtgttgc cgccatttgc acgaagaaga
                            1861 agatcgactc gtgccacgtt ggtgccgacg gctccgtctt caccaagtac cctcacttca
                            1921 aggcgcgcgg agccaaggct ctgcgcgaga tcctggactg ggctccggag gagcaggaca
                            1981 aggtgaccat catggcggcc gaggatggat ctggtgtggg agctgcgctg attgcggcgc
                            2041 tgaccctgaa gcgggtcaag gccggcaacc tggccggtat ccgaaacatg gctgacatga
                            2101 agaccctgct ataatgacct ggcgcacata aatatgaatt ctttggtgtg atattttatt
                            2161 ctctggtgtt gagacggcgg tggacggaat gaaaataatt gattacagtt gttatgaaga
                            2221 attggcccgg cctggtctgg tctggagcaa tccttgtact gattaaaaag taagccagta
                            2281 agccagtgaa tgtgtaactc tcttgttcct atcgactaga cctgccacac taccatcctg
                            2341 tagacacaaa accccgcagc aaacaagccc tgagcaatgc aagcggaagg caaaaaagaa
                            2401 agaatgtgca cggtcactca gttggccagt gttgacttgg ttgcacagga agaatgggga
                            2461 tcc



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