LOCUS YSCHXKA
2778 bp mRNA
PLN 27-APR-1993
DEFINITION Yeast (S.cerevisiae) hexokinase PI (HXK1) gene, complete
cds.
ACCESSION M14410 M11184
VERSION M14410.1 GI:171736
KEYWORDS hexokinase; hexokinase PI.
SOURCE Yeast (S.cerevisiae; strain hxk0-9C),
cDNA to mRNA, clone
YRp/HXK1-2.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Fungi; Ascomycota; Saccharomycetales;
Saccharomycetaceae; Saccharomyces.
REFERENCE 1 (bases 1 to 2778)
AUTHORS Kopetzki,E., Entian,K.D. and Mecke,D.
TITLE Complete nucleotide sequence of the hexokinase
PI gene (HXK1) of
Saccharomyces cerevisiae
JOURNAL Gene 39, 95-102 (1985)
MEDLINE 86083199
COMMENT Draft copy, computer-readable and printed
copies of sequence in [1]
kindly provided by K.-D.Entian, 06-FEB-1986.
The yeast hexokinase P-I gene has multiple transcriptional start
sites (similar to HXK2). The main site is at position 530 and
another major site is at position 583, followed by an 'atg' codon
at positions 618-620. Since proteolytic cleavage of a N-terminal
undecapeptide results in catalytically active HXK1 and HXK2, a
derivative of the HXK1 gene transcribed from the initiation site
within the main ORF may also be catalytically active.
FEATURES
Location/Qualifiers
source 1..2778
/organism="Saccharomyces cerevisiae"
/db_xref="taxon:4932"
mRNA
675..2184
/note="HXK1 mRNA (alt.)"
mRNA
675..2203
/note="HXK1 mRNA (alt.)"
CDS
718..2175
/note="hexokinase (HXK1)"
/codon_start=1
/protein_id="AAA34698.1"
/db_xref="GI:171737"
/translation="MVHLGPKKPQARKGSMADVPKELMDEIHQLEDMFTVDSETLRKV
VKHFIDELNKGLTKKGGNIPMIPGWVMEFPTGKESGNYLAIDLGGTNLRVVLVKLSGN
HTFDTTQSKYKLPHDMRTTKHQEELWSFIADSLKDFMVEQELLNTKDTLPLGFTFSYP
ASQNKINEGILQRWTKGFDIPNVEGHDVVPLLQNEISKRELPIEIVALINDTVGTLIA
SYYTDPETKMGVIFGTGVNGAFYDVVSDIEKLEGKLADDIPSNSPMAINCEYGSFDNE
HLVLPRTKYDVAVDEQSPRPGQQAFEKMTSGYYLGELLRLVLLELNEKGLMLKDQDLS
KLKQPYIMDTSYPARIEDDPFVFLEDTDDIFQKDFGVKTTLPERKLIRRLCELTGTRA
ARLAVCGIAAICQKRGYKTGHIAADGSVYNKYPGFKEAAAKGLRDIYGWTGDASKDPI
TIVPAEDGSGAGAAVIAALSEKRIAEGKSLGIIGA"
mat_peptide 721..2027
/note="hexokinase P-I mature peptide"
mRNA
728..2203
/note="HXK1 mRNA (alt.)"
mRNA
728..2184
/note="HXK1 mRNA (alt.)"
BASE COUNT 835 a 510 c
570 g 862 t 1 others
ORIGIN Sau3A site.
1 gatccaatct taaaaagaac catcatttcc aaaagaaagg tcaccaagta ataacgagga
61 tgaggagatt gtcaagacac ccagaaaatt agtcaattac gtacctttaa aaatttctca
121 atctaggtga tagcttcgac gatacgatct cagtatagca gtgacgcgtg ggtttcagga
181 agaatggcag tccccttttg tttttccgca tttgtgtgag cttcttatgc ccctgaaccc
241 actattctgc ccctttgaaa ctcccgcacg tgtgccccgt ttgttggaag atagcgaaat
301 accttactgg agcaaccagg aaaaatactc tggttgcaaa aaccaacaaa agaaaaaatg
361 ggaagaccta agactatgca ttttttaagg gttgatgaaa gaaaagttac ttctcccgga
421 ttttggtacc ttaggaccgt tgagaggaat agtaacaagt gaacgcaaca aagattgttc
481 tcaactgctt ctgtttcctc cttttcttta aagaggaata tttcgtatat aagcaatcgg
541 tttcacttcc ttgggaatat tctaccgttc cttcatcttg tattcttctc tttctcttag
601 cgcaatatat agcagaagag caataagaaa caattgtggc ttgcaatact caattagaat
661 tcttttcttt taatcaaact cacccaaaca actcaattag aatactgaaa aaataagatg
721 gttcatttag gtccaaagaa accacaggct agaaagggtt ccatggctga tgtgcccaag
781 gaattgatgg atgaaattca tcagttggaa gatatgttta cagttgacag ygagaccttg
841 agaaaggttg ttaagcactt tatcgacgaa ttgaataaag gtttgacaaa gaagggaggt
901 aacattccaa tgattcccgg ttgggtcatg gaattcccaa caggtaaaga atctggtaac
961 tatttggcca ttgatttggg tggtactaac ttaagagtcg tgttggtcaa gttgagcggt
1021 aaccatacct ttgacaccac tcaatccaag tataaactac cacatgacat gagaaccact
1081 aagcaccaag aggagttatg gtcctttatt gccgactctt tgaaggactt tatggtcgag
1141 caagaattgc taaacaccaa ggacacctta ccattaggtt tcaccttctc gtacccagct
1201 tcccaaaaca agattaacga aggtattttg caaagatgga ccaagggttt cgatattcca
1261 aatgtcgaag gccacgatgt cgtcccattg ctacaaaacg aaatttccaa gagagagttg
1321 cctattgaaa ttgtagcgtt gattaatgat actgttggta ctttaattgc ctcatactac
1381 actgacccag agactaagat gggtgtgatt ttcggtactg gtgtcaacgg tgctttctat
1441 gatgttgttt ccgatatcga aaagttggag ggcaaattag cagacgatat tccaagtaac
1501 tctccaatgg ctatcaattg tgaatatggt tccttcgata atgaacattt ggtcttgcca
1561 agaaccaagt acgatgttgc tgtcgacgaa caatctccaa gacctggtca acaagctttt
1621 gaaaagatga cctccggtta ctacttgggt gaattgttgc gtctagtgtt acttgaatta
1681 aacgagaagg gcttgatgtt gaaggatcaa gatctaagca agttgaaaca accatacatc
1741 atggatacct cctacccagc aagaatcgag gatgatccat ttgttttctt ggaagatact
1801 gatgacatct tccaaaagga ctttggtgtc aagaccactc tgccagaacg taagttgatt
1861 agaagacttt gtgaattgac tggtaccaga gctgctagat tagctgtttg tggtattgcc
1921 gctatttgcc aaaagagagg ttacaagact ggtcacattg ccgctgacgg ttctgtctat
1981 aacaaatacc caggtttcaa ggaagccgcc gctaagggtt tgagagatat ctatggatgg
2041 actggtgacg caagcaaaga tccaattacg attgttccag ctgaggatgg ttcaggtgca
2101 ggtgctgctg ttattgctgc attgtccgaa aaaagaattg ccgaaggtaa gtctcttggt
2161 atcattggcg cttaatgaaa aaaatgtaat gaaatataaa tgtgtttttc cctcccttaa
2221 tattattatt cttatgagtg atgcccttat gttttttttg cggtctagta tatgtaaata
2281 tagacacaca catatatata tatttatgtg tataatccta gctaataaac atttttagat
2341 tgttattagt ataatttttc cacttacttt gttctccggg agagaaaaag ttgtttattt
2401 tgtagtaatt ttgtgcacat attatatata caaatattac acaatatttt caatacttat
2461 ggcataatcc attgcttttt cgataatgtt tgtcttttgg tcttcttcat gcatcatttc
2521 atcttcgtaa tcagctttct cctttgattg gttattgaag taaactttcg agttgacaat
2581 aggccagttt ctctctagtg caaatttttc agtttctttt tcgttgttga aaaagagcaa
2641 agcctttatg ttggagagag ggagaaagtc gtaggatagc tcggtatttt tagcaatttc
2701 gtctcttata gctgatttta ggatatcggt aaaagagtcg aattctgata tattctgcga
2761 cccagattgc agaagatc