LOCUS       ECENO        2089 bp    DNA             BCT       15-APR-1997
     DEFINITION  E.coli eno gene.
     ACCESSION   X82400
     VERSION     X82400.1  GI:563867
     KEYWORDS    eno gene; enolase.
     SOURCE      Escherichia coli.
       ORGANISM  Escherichia coli
                 Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
                 Escherichia.
     REFERENCE   1  (bases 1 to 2089)
       AUTHORS   Klein,M., Sprenger,G.A. and Freudl,R.
       TITLE     Cloning, nucleotide sequence, and functional expression of the
                 Escherichia coli enolase (eno) gene in a temperature-sensitive eno
                 mutant strain
       JOURNAL   DNA Seq. 6, 315-355 (1996)
     REFERENCE   2  (bases 1 to 2089)
       AUTHORS   Freudl,R.
       TITLE     Direct Submission
       JOURNAL   Submitted (27-OCT-1994) R. Freudl,
                 Inst.f.Biotech.1,Forschungszentrum Julich, Forschungszentrum
                 Juelich, GmbH, D-52425, Juelich, FRG
     FEATURES             Location/Qualifiers
          source          1..2089
                          /organism="Escherichia coli"
                          /strain="K12"
                          /sub_strain="JM109"
                          /db_xref="taxon:562"
          RBS             13..17
          gene            25..1338
                          /gene="eno"
          CDS             25..1338
                          /gene="eno"
                          /EC_number="4.2.1.11"
                          /note="enolase"
                          /codon_start=1
                          /transl_table=11
                          /product="phosphopyruvate hydratase"
                          /protein_id="CAA57795.1"
                          /db_xref="GI:563868"
                          /db_xref="SWISS-PROT:P08324"
                          /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                          SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEK
                          KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                          DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                          NDEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                          LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                          IANSYLIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                          KTGSMSRSDRVAKYNQLIRIEEALGEKARTTVVKRSKARHKTDFI"
     BASE COUNT      605 a    485 c    497 g    502 t
     ORIGIN
             1 aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa aaatcatcgg tcgtgaaatc
            61 atcgactccc gtggtaaccc gactgttgaa gccgaagtac atctggaggg tggtttcgtc
           121 ggtatggcag ctgctccgtc aggtgcttct actggttccc gtgaagctct ggaactgcgc
           181 gatggcgaca aatcccgttt cctgggtaaa ggcgtaacca aagctgttgc tgcggtaaac
           241 ggcccgatcg ctcaggcgct gattggcaaa gatgctaaag atcaggctgg cattgacaag
           301 atcatgatcg acctggacgg caccgaaaag aaatccaaat tcggcgcgaa cgcaatcctg
           361 gctgtatctc tggctaacgc caaagctgct gcagctgcta aaggtatgcc gctgtacgag
           421 cacatcgctg aactgaacgg tactccgggc aaatactcta tgccggttcc gatgatgaac
           481 atcatcaacg gtggtgagca cgctgacaac aacgttgata tccaggaatt catgattcag
           541 ccggttggcg cgaaaactgt gaaagaagcc atccgcatgg gttctgaagt tttccatcac
           601 ctggcaaaag ttctgaaagc gaaaggcatg aacactgctg ttggtgacga aggtggctat
           661 gcgccgaacc tgggttccaa cgatgaagct ctggctgtta tcgctgaagc tgttaaagct
           721 gctggttatg aactgggcaa agacatcact ttggcgatgg actgcgcagc ttctgaattc
           781 tacaaagatg gtaaatacgt tctggctggc gaaggcaaca aagcgttcac ctctgaagaa
           841 ttcactcact tcctggaaga actgaccaaa cagtacccga tcgtttctat cgaagacggt
           901 ctggacgaat ctgactggga cggtttcgca taccagacca aagttctggg cgacaaaatc
           961 cagctggttg gtgacgacct gttcgtaacc aacaccaaga tcctgaaaga aggtatcgaa
          1021 aaaggtatcg ctaactccta cctgatcaaa ttcaaccaga tcggttctct gaccgaaact
          1081 ctggctgcaa tcaagatggc gaaagatgct ggctacactg cagttatctc tcaccgttct
          1141 ggcgaaactg aagacgctac catcgctgac ctggctgttg gtactgctgc aggccagatc
          1201 aaaactggtt ctatgagccg ttctgaccgt gttgctaaat acaaccagct gattcgtatc
          1261 gaagaagctc tgggcgaaaa agcacgtaca acggtcgtaa agagatcaaa ggccaggcat
          1321 aagactgact ttatctgatt taaaaactag ctccgggctg gcatttttta tggttgagtt
          1381 accacgggcc ggatgcgccg ccacctccag aagaaccgcc cccgccgcta aatccgccgc
          1441 tatctgagcc tccagcggaa ccgctgttgt ctgaatgata acttctacca gatgctcttt
          1501 tctgaagtac acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa
          1561 atacagcaat ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg
          1621 aaatacagac tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga
          1681 aagggaagaa catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag
          1741 acgctgattc actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat
          1801 tcaaagcgtt tatagctagc tctaatccct tagctaattt ttgttgttta aatgcgggta
          1861 tcatgttgct acggatgata tctccagcca gagcatcggt taccttttct tccagcccat
          1921 aacctacctg gatgcggaca gtgcgatccg accaggcaac aacgatcagt atcccatcat
          1981 tacgtttggc atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg
          2041 tctcgtcttt ggttgtggga acaattaata cggcaatttg cacttggaa
     //