Figure 1.    Depiction of complementary DNA sequence from E. coli Era gene.  The largest ORF lies in the first reading frame and spans the entire region from nucleotide 1-906.  Original nucleotide sequence:  E. Coli sequence    Predicted molecular weight of E. Coli Era:   33808 Daltons.

Figure 2.  Hydrophibicity plot suggestive of four possible transmembrane domains where plot values exceed 2.00.  Therefore, Era is possibly an integral membrane protein.

Figure 3.  An important characteristic of epitopes is that they are exposed in order to interact with antibodies when the molecule is in its native conformation.  Oligopeptides from regions where the plotted values are continuously over 0.00 such as from amino acids 40-60 or 220-240 are predicted to be hydrophilic and consequently would be suitable for antibody generation.

Figure 4.  Predicted secondary structure of Era.

Figure 5.  Gapped alignment by Higgins method.  Conserved amino acids are highlighted in black.  Links to: C. elegans, E. coli, D. Melanogaster, H. sapiens, S. cerevisiae  amino acid sequences.

[Davidson Molecular Biology web page]

Email any questions, comments or suggestions to: fisturgill@davidson.edu