LOCUS       DROPFK       7101 bp    DNA             INV       23-FEB-1995
DEFINITION  Drosophila melanogaster phosphofructokinase (pfk) gene, complete
            cds.
ACCESSION   L27653
NID         g514918
KEYWORDS    phosphofructokinase.
SOURCE      Drosophila melanogaster (strain Oregon) adult DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Arthropoda;
            Tracheata; Insecta; Pterygota; Diptera; Brachycera; Muscomorpha;
            Ephydroidea; Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Currie,P.D. and Sullivan,D.T.
  TITLE     Structure and expression of the gene encoding phosphofructokinase
            (PFK) in Drosophila melanogaster
  JOURNAL   J. Biol. Chem. 269 (40), 24679-24687 (1994)
  MEDLINE   95014229
COMMENT     On Jul 23, 1994 this sequence version replaced gi:471780.
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Drosophila melanogaster"
                     /strain="Oregon"
                     /db_xref="taxon:7227"
                     /dev_stage="adult"
     TATA_signal     698..704
     exon            719..1048
                     /number=1
     5'UTR           719..901
     gene            join(902..1048,2070..2414,2472..2635,3008..3110,
                     4291..4476,4663..5102,5160..5999,6079..6217)
                     /gene="pfk"
     CDS             join(902..1048,2070..2414,2472..2635,3008..3110,
                     4291..4476,4663..5102,5160..5999,6079..6217)
                     /gene="pfk"
                     /codon_start=1
                     /product="phosphofructokinase"
                     /db_xref="PID:g514919"
                     /translation="MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAI
                     YLGCKVYFIREGYQGMVDGGDCIRKSNWASVSSIIPFVGWHHPLLRPLPDFRERQGRL
                     KAANNLIQRGITNLVVIGGDGSLTGANLLPQEWSSLLDELVKNKTITTEQQEKFNVLH
                     MLGLVGSIDNDFCGTDMTIGTDTALHRIIEQSKAKVQSPVQPNSHQRTFIMEGQVSHC
                     GYLALVGGLACEAIHIHPNAPGRLGQTGSALSWTQRSAGQRLNIVIVAEGAMDREGHP
                     ITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRFLACRMGAEPLWPLMEATKDS
                     VPVVISLDGNQAVRVPLMECVERTQAGKAMAEKRWADAVNVRGRSFERNLETYKMLTR
                     LKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVE
                     GLIARNVRELGWSDVSGWVGQGGAYLGSKRTLPEGKFKEIAARLKEFKIQGLLIIGGF
                     ESYHAAGQIADQRTTTHSFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIR
                     QSAQGTKRRVFVIETMGGYCGYLPPLAGLAGGADAAYIYEEKFSIKELQQDVYHMPPR
                     WPRRLPRSNPANEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRN
                     MGTKMAAKCVDCWPPRSRRTSTANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETN
                     FDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA"
     intron          1049..2069
                     /number=1
     exon            2070..2414
                     /gene="pfk"
                     /number=2
     intron          2415..2471
                     /number=2
     exon            2472..2635
                     /gene="pfk"
                     /number=3
     intron          2636..3007
                     /number=3
     exon            3008..3110
                     /gene="pfk"
                     /number=4
     intron          3111..4290
                     /number=4
     exon            4291..4476
                     /gene="pfk"
                     /number=5
     intron          4477..4662
                     /number=5
     exon            4663..5102
                     /gene="pfk"
                     /number=6
     intron          5103..5159
                     /number=6
     exon            5160..5999
                     /gene="pfk"
                     /number=7
     intron          6000..6078
                     /number=7
     exon            6079..>6217
                     /gene="pfk"
                     /number=8
     polyA_signal    6452..6457
     polyA_signal    6545..6550
     polyA_signal    6773..6778
BASE COUNT     1809 a   1651 c   1623 g   2018 t
ORIGIN
        1 agcttataga ttcagcttgt accatgccag gaataacatg aggattaacg cgcacgttca
       61 tttctgggtc tgtcaccggc gcggctaaat ttacaacttg ctgccagaat gtgccgccaa
      121 gaaccgcggt ttgtgccgga cgcacaacgc cttaacccaa ggcaaccaag ctgcagctgc
      181 ctcctccaag caccaccgga gtgcccgcct tagcagccag cagtttccgc cgctttgtga
      241 acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat cggtcatcgt
      301 aggccagcca tatccagcaa tgcaggtttc cagtcacggg tggttagatc aagaagtccc
      361 gtggtgccag cgttagaggg atcaacagct gaacgattgt gggtcgaacg taaacaaatt
      421 ggttaaacgc tattttccgc tcgttgctag tcgcgaaagt cgttcataac agacagaatc
      481 ctcagatttt cgacctaaat caaaccaagt tagggcaatc gcgttggagc gaactcttgg
      541 gcggatttag ccatccgaag tttgcattgc atcagtacgc acttacagaa agcccgtggc
      601 tgtgtgctgt gtccgtgtgt gttgtgtatg cgtctctggc ctgaaggtca ccttgaagct
      661 caaagcaaag cgaactgtga ataccgcctg cgagcggtat atattgctag taactgaaaa
      721 gccacggcaa ggagacgtag tagtagtatt tatagcccac ttgatataac ccagctccag
      781 cggcgctgct cgcatagttg caaaactccc taccgagtac aatagtggtt catagtgtgt
      841 gctttgtgcg atatccgtta cgaccccagt ttgatttaca tatagtgcaa aagattgaaa
      901 aatgaattcc gagattaatc aacgattcct ggcccgcggc tcgcagaagg acaaaggact
      961 ggcggtcttc accagcggcg gggattccca gggcatgaat gccgccgtgc gagcctgtgt
     1021 ccgtatggcc atctatttgg gctgcaaggt gagttggtga cctctgtcaa gtacttccgc
     1081 ccacgcagat cgcgtccggt gaccccgatc ccaggcacag gcaacaaacc catagtgaac
     1141 ccagaaattt cgtgaaacac ctggtaattt gcttatcagc agccgttaaa ccacgattat
     1201 tgggccgcaa agccagttag ataatctcca atcggtgcga aaagaaattc aagaatatat
     1261 gtgtacatgc acattgacat tgggtttatt ttcgggtcct cacatgcgat tttcgatttt
     1321 cggctaattt gtttcgtttt tttgttgtcg ctcttggttt ctattcggtt tatttaaatg
     1381 agttctttaa tttgttaggg aacttcttgc ccggccgttg gctccctcga aatatcgaaa
     1441 atgtgtcaat taacgtagcc aatttaaatt gataaactat tcatttgatt caatttctgg
     1501 ggcataaagt aaagtattgc tgccttgcat gaccagcgct tgaatatggt tcaataaatt
     1561 gacaaattaa taaatatatc atggtatgct aaacaaatgg ttcctaatca gttcaataat
     1621 ctaacctttg tttttaatct actagtgcct tggcatcatt attgtttatg ttattttttc
     1681 acttttcaaa ttaagagcgt tataaaattt gaattccctt ggttatctga gttgcttaaa
     1741 aagtatcgat ttaacatcga ttacattgta attacctgta acggtatttt gaatatgcaa
     1801 atgttttgtt gtttaaaact taaacttatc agtaaaagtt gagaaaagta tatgagataa
     1861 gtcatatcat attaatttct attgactgtt ctttactgac ttcctattaa atattacgac
     1921 tttggcagag tgaaatgaaa ttaaataatt tatactcgaa ttcggtgatt catttttaat
     1981 tgtgctaatg atgtcatgtg ccatgaagat accaaactct attctgtttt acaccatatc
     2041 tacaattgat tcgtgtttac ttcggcaagg tctacttcat tcgtgaaggc taccagggca
     2101 tggtggatgg tggcgactgc atcaggaagt ccaactgggc ctcagtctca tccattattc
     2161 cattcgttgg ttggcaccat ccattgctcc gccctttgcc agacttccgt gagcgtcagg
     2221 gtcgcttgaa agccgctaac aacctgattc agcgaggaat caccaatctg gtggtcattg
     2281 gaggcgatgg ctccctcacg ggcgccaatc tgttgcctca ggagtggtcc agcctgttgg
     2341 acgagctggt caagaacaag accattacca ccgagcagca ggaaaagttc aatgtcctgc
     2401 acatgcttgg attggtatgc tacacagagt attccttgga tatattttct tatggatgaa
     2461 tctttttgta ggttggctcc atcgacaacg atttctgtgg cactgacatg accatcggca
     2521 cggacacggc actgcatcgc atcatcgagc aatcgaaggc taaggtccaa tctccagtac
     2581 agcctaactc ccatcagcgc accttcatca tggaaggtca ggtgtctcat tgcgggtaag
     2641 ttgatctctg agcggatatt gcgaggaaaa gttggatgcc tctgtattag cctaagtgtc
     2701 agtgattaaa ttgggatttt agctaatgcc tggaacgact atctctagct atctttcggt
     2761 ggtagctggc attatctccg aaggcaagac tacgtctttc tgcccgagtc tcctccgcag
     2821 ccgattggcc cgatcgactt gtccttaaat tggaacaggc aagcccaaaa cggaaatctg
     2881 ttgaatgtcg tcgactcatt tcgcacattt ctgtttctat ggccgcagta ctatgtcgat
     2941 cgatccccat atcctccaaa atcccatccc ccttttgact aatattttgt aatatatatt
     3001 tttgcagcta tttagctctt gtgggtggac tggcgtgcga ggcgattcat attcatccga
     3061 atgcgccagg tcgattaggc cagacaggct ctgctctcag ctggacccag gtcaggaaga
     3121 tgagctccat ttccattcca tcaagtcact ggagaagtcc gaaaggcatc ggaaatatgt
     3181 tctcgagaga cttggtatag gatttaggat attcttatta atatagccag ttagctgtcg
     3241 aagataatat tcctgtttga gatagagttg tatacttcag tgcagttgtc gttgcttgat
     3301 gcttaagtag tacacctgta cacaattttt tgaaagacac attttaagag cgacacttgc
     3361 catacaaagc ttggttctta tttctgctta ctgcacacga atgtatcttt aaacacaaac
     3421 cggagatacg gaatgaatta cgcacagttt ttgatctgta ccagcataca aaatcatatg
     3481 taactaactt aagtttaccc agatactcca atcccggata atatctcccg acattgtgaa
     3541 ccatctttat tttgtaccta actcttgttt ttagttacta gccatatcgg cagccaatag
     3601 gccacagagg ctgatttatg ttcatacccg agagccggtg tcagtcaatt ggaaggacag
     3661 atctgtgtca agctccacca ggtcaggtcc agagtccttc gctatccaat taatttctag
     3721 ctactctttt gtcaaatttt tgaatttcta aattttattc tgcattgttt ttggactatt
     3781 cgatgtgccg aaatttccat ggtggcgata aacagcttta acagctattt gatttacttg
     3841 tttgggtttt cggtctctgt ttttgagtac cactatgatc tcactgagtt cccttactaa
     3901 cttttccaaa tccactaacg tccatcctcg ttaatcgatc gtaaatcttc atcaatgttt
     3961 ctattcatcg acttgactga tcggctaagg atcggctcaa gtcaacgtta atctaatcat
     4021 gaagcccgca ttatgatcga actgaatcat gaatatcata gaataacgtg agtactttag
     4081 aacgcatgca ctcatcgctt gctgatacac atttggtgac actattcaac attgtaagca
     4141 aagcatatgt aaactacatt attgtatgat gatgtctact catcatgccc tcttcataat
     4201 tcgtttgtgc ttttgtagtt tctgtatcat ccacgattag tatcttacat atatgcttaa
     4261 atgaatccct tcaccattga ccaacaggag cgatctgctg gccagcgtct gaacatcgtg
     4321 attgtggccg agggagccat ggatcgcgag ggtcatccca tcacagccga ggatgtaaag
     4381 aaggtgatcg acgagcgtct gaagcacgat gcccgcatca ctgtcttggg tcacgtgcag
     4441 cgcggtggca atcccagtgc cttcgatcgt tttttggtaa gtatttatac gaagatgacc
     4501 tcaataactg tatttttagt aatttacaat gctagctagt tttagattgt atcttttaaa
     4561 ggttaatatt aaaacactaa agttaccaaa ataaggatat acttattaag atatgattca
     4621 atctattttt gacttgagag ttaatgcttc taccccgcat aggcttgccg catgggagct
     4681 gagccacttt ggcccttgat ggaggccacc aaggactcgg tgccagtggt catctctctg
     4741 gacggcaacc aggcggttcg cgtgccgctg atggagtgcg tggagcgcac ccaggctggc
     4801 aaggccatgg cggagaaacg ctgggcggat gccgtcaacg tgcgcggacg ctccttcgag
     4861 cggaatctgg agacctacaa gatgctgacg cgcttgaagc cgcccaagga gaacttcgac
     4921 gccgatggca agggaatcga aggataccgc ctagctgtga tgcatattgg tgctccggct
     4981 tgcggtatga atgccgctgt gcgcagcttc gtgcgcaatg ccatctaccg cggtgatgtg
     5041 gtttatggaa tcaacgacgg tgtcgagggt ctgattgcgc gaaatgtccg cgagctgggc
     5101 tggtaagtaa tccgacaagg gatcactcta aatcgtgtct taatgcaatt caatttcagg
     5161 tcggatgtct caggatgggt tggtcagggt ggcgcctact tgggatccaa acgcacactg
     5221 cctgagggca agttcaagga gatcgccgct cgtctcaagg agtttaagat ccagggtctc
     5281 ctgatcattg gtggctttga gagttaccat gccgccggac agatcgccga tcagcggaca
     5341 actacccaca gcttctgcat ccccattgtg gttattccat cgacgatttc gaacaatgtg
     5401 cccggcacag aattttctct gggatgcgac accggtttaa atgagattac ggagatttgc
     5461 gaccgtatcc gtcagtcggc acagggaacc aagcgccgag tgttcgtcat tgagacgatg
     5521 ggtggctact gtggctattt gccaccgttg gccggtttgg ccggcggagc tgatgccgcc
     5581 tacatctacg aggagaagtt ctccatcaag gagctgcagc aggatgtcta ccacatgcct
     5641 ccaagatggc cgaggcgtct cccgcggtct aatcctgcga acgagaaggc tagcgagaac
     5701 tacagcacgg acttcattta ccgcctgtac tcggaggagg gcaagggcct cttcacctgc
     5761 cgtatgaaca tcctgggtca catgcagcag ggcggctcac ccactccctt cgaccgcaac
     5821 atgggcacca aaatggccgc caagtgtgtc gactgctggc cgcccagatc aaggcgaaca
     5881 tcgacggcca acggtgtggt caactgcaag tccccggaca ccgccacgct gctgggaatc
     5941 gtgtcgcggc agtaccgctt ctcaccgctg gtcgacctca ttgcagagac gaacttcgag
     6001 taagtttaac ccgatggtca ccacaaaggg gactatgaat tccctttaag ctcttggcat
     6061 aacccactcc tcttccagtc aacgcatccc gaagaagcag tggtggctgc gcctgcgtcc
     6121 cctgctgcgc atcctggcca agcacgattc cgcctacgag gaggagggta tgtacatcac
     6181 cgtcgaggag gagtgtgaca ctgacgccgt cgcctaagcg gactcggatc cggactctga
     6241 tccccacttc cgcttatcac caccatcgct gcatgcaagc atcaatgtcc acctatccgc
     6301 accctcgaat cagtctctgt gttcgctttt taagttgttt tggcaccgca tccgatccgt
     6361 tttatggttt ttaccaagat cagacgaaga ggcccatgac agcagcacat acgaaagtat
     6421 ttcataatct acagacgcat aaaaaagcac aaatacataa cttatttata ttgctcgcca
     6481 gtcgattctt tcttatgatt tttccaaata tcgtgtattc ttgaaatttg aatctcgaat
     6541 actgaataac gttaatagtt cattctacat tgcagccgtt ccgaagagtc tggcgaccta
     6601 ttagcggaaa acgtttcgta tattatcgta attgttggtt cttcagccca actaaattaa
     6661 atgttaaagt atttagacaa tttacccagt caaaggaaca aattctatct gcaaactgtt
     6721 tttcgagtat tgttatcgaa cgaagcaatg atctatttgt aacgacgtgc taaatacata
     6781 tcaaacacct gttggcacaa tttatcgtcg tttgttttct aattgttcag taggtatgtg
     6841 ggaattgagc ttgaatttca cttttggtta agaaaacatt tatttttgaa atggcttgct
     6901 gggcgcgatt ggaacagcaa tgtttcagtg gaatcagcct ataatacgat gtttctgcag
     6961 caacttcact gcttgcaagc tatcggctca atcgattgtg gtgccatcga tgcgcttcca
     7021 gcctagacac ttgacttttt gcacggatgt gtatgtagtt tattgtgctc gaaactgcgt
     7081 aagtgatgtc agcgtgactg a