LOCUS 1346355
784 aa
01-FEB-1996
DEFINITION 6-PHOSPHOFRUCTOKINASE, TYPE C (PHOSPHOFRUCTOKINASE
1)
(PHOSPHOHEXOKINASE) (PHOSPHOFRUCTO-1-KINASE ISOZYME C)
(6-PHOSPHOFRUCTOKINASE, PLATELET TYPE).
ACCESSION 1346355
PID g1346355
DBSOURCE SWISS-PROT: locus K6PP_HUMAN, accession
Q01813
class: standard.
created: Jul 1, 1993.
sequence updated: Feb 1, 1996.
annotation updated: Feb 1, 1996.
xrefs: gi: 464186, gi: 560105, gi: 189852, gi: 189853, gi: 86719
xrefs (non-sequence databases): HSSP P00512, MIM 171840, PROSITE
PS00433
KEYWORDS KINASE; TRANSFERASE; GLYCOLYSIS; REPEAT;
ALLOSTERIC ENZYME;
PHOSPHORYLATION; MAGNESIUM; MULTIGENE FAMILY.
SOURCE human.
ORGANISM Homo sapiens
Eukaryotae; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria;
Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 784)
AUTHORS Eto,K., Sakura,H., Yasuda,K., Hayakawa,T.,
Kawasaki,E.,
Moriuchi,R., Nagataki,S., Yazaki,Y. and Kadowaki,T.
TITLE Cloning of a complete protein-coding
sequence of human
platelet-type phosphofructokinase isozyme from pancreatic islet
JOURNAL Biochem. Biophys. Res. Commun. 198 (3),
990-998 (1994)
MEDLINE 94161770
REMARK SEQUENCE FROM N.A.
TISSUE=PANCREATIC ISLETS
REFERENCE 2 (residues 1 to 784)
AUTHORS P6Simpson,C.J. and Fothergill-Gilmore,L.A.
TITLE Isolation and sequence of a cDNA
encoding human platelet
phosphofructokinase
JOURNAL Biochem. Biophys. Res. Commun. 180 (1),
197-203 (1991)
MEDLINE 92028938
REMARK SEQUENCE OF 484-784 FROM N.A.
COMMENT On Jun 1, 1996 this sequence version
replaced gi:400096.
[CATALYTIC ACTIVITY] ATP + D-FRUCTOSE 6-PHOSPHATE = ADP +
D-FRUCTOSE 1,6-BISPHOSPHATE.
[COFACTOR] REQUIRES MAGNESIUM ION.
[ENZYME REGULATION] ALLOSTERIC ENZYME ACTIVATED BY ADP, AMP, OR
FRUCTOSE BISPHOSPHATE AND INHIBITED BY ATP OR CITRATE.
[PATHWAY] KEY CONTROL STEP OF GLYCOLYSIS.
[SUBUNIT] TETRAMER. MUSCLE IS M4, LIVER IS L4, AND RED CELL IS M3L,
M2L2, OR ML3. A SUBUNIT COMPOSITION WITH A HIGHER PROPORTION OF
PLATELET TYPE SUBUNITS IS FOUND IN PLATELETS, BRAIN AND
FIBROBLASTS.
IN HUMAN PFK EXISTS AS A SYSTEM OF 3 TYPES OF SUBUNITS, PFKM
(MUSCLE), PFKL (LIVER) AND PFKP (PLATELET) ISOENZYMES.
FEATURES
Location/Qualifiers
source
1..784
/organism="Homo sapiens"
/db_xref="taxon:9606"
1..784
Protein
1..784
/product="6-PHOSPHOFRUCTOKINASE, TYPE C"
/EC_number="2.7.1.11"
Region
484..485
/note="PG -> IP (IN REF. 2)."
/region_name="Conflict"
Region
498
/note="MISSING (IN REF. 2)."
/region_name="Conflict"
Region
699
/note="A -> E (IN REF. 2)."
/region_name="Conflict"
ORIGIN
1 mdaddsrapk gslrkflehl
sgagkaigvl tsggdaqgmn aavravvrmg iyvgakvyfi
61 yegyqgmvdg gsniaeadwe svssilqvgg
tiigsarcqa frtregrlka acnllqrgit
121 nlcviggdgs ltganlfrke wsglleelar
ngqidkeavq kyaylnvvgm vgsidndfcg
181 tdmtigtdsa lhriievvda imttaqshqr
tfvlevmgrh cgylalvsal acgadwvflp
241 esppeegwee qmcvklsenr arkkrlniii
vaegaidtqn kpitsekike lvvtqlgydt
301 rvtilghvqr ggtpsafdri lasrmgveav
ialleatpdt pacvvslngn havrlplmec
361 vqmtqdvqka mderrfqdav rlrgrsfagn
lntykrlaik lpddqipktn cnvavinvga
421 paagmnaavr savrvgiadg hrmlaiydgf
dgfakgqike igwtdvggwt gqggsilgtk
481 rvlpgkylee iatqmrthsi nalliiggfe
aylgllelsa arekheefcv pmvmvpatvs
541 nnvpgsdfsi gadtalntit dtcdrikqsa
sgtkrrvfii etmggycgyl anmgglaaga
601 daayifeepf dirdlqsnve hltekmktti
qrglvlrnes csenyttdfi yqlyseegkg
661 vfdcrknvlg hmqqggapsp fdrnfgtkis
aramewitak lkeargrgkk fttddsicvl
721 giskrnvifq pvaelkkqtd fehripkeqw
wlklrplmki lakykasydv sdsgqlehvq
781 pwsv