LOCUS BOVPHOSC 4651 bp mRNA MAM 20-AUG-1993
DEFINITION Bovine phospholipase C mRNA, complete cds.
ACCESSION L13937
NID g304242
KEYWORDS phospholipase; phospholipase C; retinal
protein; signal
transduction.
SOURCE Bos taurus (library: lambda ZAP
(Stratagene) and lambda gt10)
Female Adult Retina cDNA to mRNA.
ORGANISM Bos taurus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
Vertebrata; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovoidea;
Bovidae; Bovinae; Bos.
REFERENCE 1 (bases 1 to 4651)
AUTHORS Ferreira,P.A., Shortridge,R.D. and Pak,W.L.
TITLE Distinctive subtypes of bovine
phospholipase C that have
preferential expression in the retina and high homology to the
norpA protein of Drosophila
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 90, 6042-6046
(1993)
MEDLINE 93317620
FEATURES
Location/Qualifiers
source
1..4651
/organism="Bos taurus"
/db_xref="taxon:9913"
/dev_stage="Adult"
/sex="Female"
/tissue_type="Retina"
/tissue_lib="lambda ZAP (Stratagene) and lambda gt10"
CDS
487..3210
/standard_name="bovine retina PLC beta; class II, form A"
/note="preferentially expressed in bovine retina;
homologue of norpA gene of Drosophila"
/codon_start=1
/function="retina signal transduction"
/evidence=experimental
/product="phospholipase C"
/db_xref="PID:g304243"
Translation="MQIIEMYEPDEDLKKQGLISSDGFCRYLMSDEN
APVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQV
LLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFV
TSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRP
LPSPNDLKRKILIKKQTTETEVEKKQLEALKSMMEAGESAAPVNMLEDD
NEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDE
QAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMS
SFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWN
SGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDP
FSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRT
RMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLD
GLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKK
FLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSS
SELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELSS
LKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSN
CLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEIRDLLL
SQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDK
SIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEF
VEKQNEQAKEMQQMVKLEAEMDRRPATVV"
polyA_site 4651
BASE COUNT 1516 a 978 c
1020 g 1137 t
ORIGIN
cDNA
1 caatggcgtg tgtcttcaag
tagcccaggg gactgtctgt cttagaatca agtcacctga
61 ctggcacaac cagctctggt cagcagaggg
ccatttcagg agccagtttg gggagtgaaa
121 aatacccaga aaggagaagt gattttattt
tcctttttct tttcttctat cccaccatct
181 tggatctttg catcacagaa aacagaatta
atagcaactt ttttttttcc ttttctgccc
241 tagtattact agaacctttg catcggggaa
aacagaaaag gtgatctttc aagcactcaa
301 ggagctaggt cttcccagtg gaaagaatga
tgaaattgag cctgccgcat ttacttatga
361 aaagttctat gaactgacac aaaagatttg
tcctcggaca gatatagagg atcttttcaa
421 aaaaatcatc aacgagatcc tcgactgaat
gaaattttat tcccatttta tgatgctaaa
481 agggcaatgc agatcattga gatgtatgag
cccgatgaag atttgaagaa acaaggcctc
541 atatcaagtg atgggttttg cagatacctg
atgtcagatg aaaacgcccc cgtcttccta
601 gatcgtctag agctgtacca agaaatggac
caccctctgg ctcactactt catcagctct
661 tcccacaaca cctatctcac tggcagacag
tttggtggga aatcttcagt agaaatgtac
721 agacaggtcc tcctggctgg ttgcagatgt
gttgaacttg actgttggga tggaaaaggt
781 gaggaccagg aaccgataat aactcacggg
aaagcaatgt gcacagacat cctttttaag
841 gatgttattc aagccatcaa ggaaactgcc
tttgtcacat cggaatatcc tgtaattctc
901 tcctttgaaa atcactgcag caaatatcaa
cagtacaaga tgtccaaata ctgtgaagat
961 ctatttggag atctcctgtt gaaacaagca
cttgaatcac atccactgga accaggcaga
1021 cccttgccat ccccaaatga cctcaaaaga aaaatactca
tcaaaaagca aacgactgaa
1081 accgaggttg aaaaaaaaca gcttgaagct ttgaaaagca
tgatggaagc cggagaatcc
1141 gctgccccgg tgaacatgtt agaggatgat aatgaagagg
aaatagaaag tgctgagcaa
1201 gaggaggaag cccaccctga atacaaatat ggaaatgaac
tttctgcaga tgacttgggt
1261 cacaaggaag ctattgccaa tagtgtcaaa aagggcctgg
ttaccgtaga agatgagcag
1321 gcgtggatgg catcttataa atatgtgggt gctaccacta
atatccatcc gtatttgtcc
1381 acaatgatca actatgcaca gcctgtaaag tttcaaggtt
tccatgtggc tgaagaacgc
1441 aatattcatt ataacatgtc ttcctttaat gagtcagtcg
gtctaggcta cttgaagaca
1501 cacgccattg aatttgtgaa ttataataaa cggcaaatga
gtcgcattta ccccaaggga
1561 ggccgagtgg actccagtaa ttacatgcct cagattttct
ggaactctgg ctgccagatg
1621 gtttcgctga actatcaaac cccagattta gcgatgcagt
tgaatcaggg gaaatttgag
1681 tataatggat cgtgtgggta ccttctcaaa ccagatttca
tgagacggcc tgatcgaaca
1741 tttgatcctt tctctgaaac gcctgttgat ggggttattg
cagccacttg ctccgtgcag
1801 gtcatatcag gtcagttctt atcagataag aaaattggca
catatgtaga agtggatatg
1861 tatgggctgc ccacggacac tatccggaag gagttccgca
ctcgcatggt gatgaacaac
1921 ggactcaatc cggtttacaa tgaagaatca tttgtgtttc
ggaaggtgat cctccccgac
1981 ctggctgtct tgaggatcgc cgtgtatgat gacaacaaca
agctgatcgg ccagaggatt
2041 ctgcccctgg atggcctcca agcaggctat cgacacatat
ccctccggaa tgagggaaat
2101 aaacctttat cactgccaac cattttctgc aatattgtcc
taaaaacata tgtgcctgat
2161 ggattcggag atattgtgga tgctttatca gatccaaaga
aatttctttc cattacggaa
2221 aagagagcgg atcaaatgag agctatgggc attgaaacta
gtgacattgc cgatgtgccc
2281 agtgacactt caaagaacga caagaaaggg aaagccaaca
cagccaaagc gaatgtgacc
2341 cctcagagca gctcagagct caggccaacc accacagctg
ccctgggggc tggcctggag
2401 gccaagaaag gtattgaact gatccctcaa gtgaggatag
aagatttaaa gcagatgaag
2461 gcttacttga agcatttaaa gaaacaacag aaagagctca
gttctttaaa gaagaaacat
2521 gcaaaggagc acagtaccat gcagaaactc cattgcactc
aagttgacaa gattgtggca
2581 cagtatgaca aggagaaatt gactcacgag aaaatcctag
agaaggcaat gaagaagaaa
2641 gggggaagta attgtcttga aatgaagaaa gaaacagaaa
ttaaaattca gacgctgaca
2701 tcagatcaca aatctaaggt caaggagatt gtggcacagc
acacaaagga gtggtcagac
2761 atgatcaaca cccacagtgc ggaggagcag gagatccggg
acctgctcct cagccagcag
2821 tgcgagctgc tcagaaagct cctgatcagc gcccacgagc
agcagaccca gcagctgaaa
2881 ctgtcccatg acagggaaag caaagagatg cgagcacacc
aagctaagat ttctatggaa
2941 aatagcaaag ccatcagcca agataaatcc atcaaaaata
aagcagaacg ggaaaggaga
3001 gtcagggagt taaacagcag caacactaaa aagtttctgg
aagaaagaaa gcgactcgcc
3061 atgaagcagt ccaaagaaat ggatcagttg aaaaaagtcc
agcttgagca cctcgaattc
3121 gtagagaaac agaatgagca ggcgaaggag atgcagcaga
tggtgaaatt ggaagccgag
3181 atggaccgca gaccagcaac agttgtgtga aactccagga
cacaaactga aaccacagaa
3241 ccaccgcatc aaagagcact ccccagcttc cgaacacaga
ttccacggtg gaaagctgat
3301 gtcttctgcg tttccttttc atgtagacaa agggtcacct
gaaaccacag agactcactt
3361 ctcataaaca ttctgaacac taaatttcct tggccaacca
aaagtagtta caaatccccc
3421 aaattgcgac tccagtaagt cagagtttac ctgttgatca
taccacttca acatgtttgc
3481 tagaaaatat catcacaagt aaatgagctt ggtgttaaaa
actctgcttt gtgatgcgtt
3541 aagacatgtt tgagcagcag caaaagtagt gcattagcca
tgtaatagca agcacatgca
3601 ctggggttgg ggggagggaa actgtctaca gatttattag
cagcagtggt gatctgctag
3661 acaaataact ggggggcatt tttctacatc tgagttacct
catcagtaag tgccacgtct
3721 ccaccatgcc atacgaagct aatttcctgt aacagtcatg
gaaatggtta gaacaacaga
3781 aaaatagaac tgtgtccttg tgccaaaacc cgctgtgctc
acaaggagaa ctctcaggca
3841 cgtttaagaa gctgacatct gacattgctt cctaggaatt
gcttctgctg accctagatt
3901 actacagctc actcttacac cgtgaagatt gtgaagtggt
tattggcaac cgtttgtaaa
3961 tgtgaccatg taaaaagtat ttatgctcct aattcacact
gtcagagagc aaaatcatgt
4021 tacgtattgc cacatgacaa gattagtgac caggaataac
tatgtttgca tgacacacaa
4081 gcaccaatta tgactaatcc gtacacagtt aacctaatgc
aaatacatac tggttaagta
4141 actgtatggc acagaatata atttgactca caagactttt
agcataatga aaaagtctat
4201 atatatgtgt atatgaacta tgtgggcatt cttgatactt
caagttctag tttcaaaaaa
4261 aatacataac taatttaatt ttacacaaaa aatatttatg
cagattttca gaatttcata
4321 tcaggaaata actttttatg tctgttaaat atcaaaacaa
tttgctacag tgttaatctg
4381 catggtcgtt aagcctgctg tagttgcgtt gcagagagtg
catggaaaag tccctcctgg
4441 gatggagctg tgccccaatg cccagaggag cgcatggaaa
ctgcacttga gaactaatca
4501 gatcactgat ttagggcacc aaccaccaac cagattgttg
tatatgcttg tacagattga
4561 ttctgtgtgt tcctctgagt aaaacagaga attatgttac
catcaatatt gaaattaaac
4621 ttccaacttc tctaataaaa aatgaaaaca c