Saccharomyces cerevisiae

LOCUS       YSCPLC1      2643 bp    DNA             PLN       29-AUG-1997

DEFINITION  S. cerevisiae phospholipase C gene, complete cds.
ACCESSION   D12738
NID         g287611
KEYWORDS    phospholipase C.
SOURCE      Saccharomyces cerevisiae (strain:X2180-1A) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota;
            Hemiascomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Yoko-o,T., Matsui,Y., Yagisawa,H., Nojima,H., Uno,I. and Toh-e,A.
  TITLE     The putative phosphoinositide-specific phospholipase C gene, PLC1,
            of the yeast Saccharomyces cerevisiae is important for cell growth
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 90 (5), 1804-1808 (1993)
  MEDLINE   93189586
COMMENT     Submitted (29-JUL-1992) to DDBJ by:
            Takehiko Yoko-o
            Dept. of Biology, Faculty of Science
            The University of Tokyo
            3-1,Hongo  7-chome
            Bunkyo-ku, Tokyo 113
            Japan
            Phone:  03-3812-2111 x4467
            Fax:    03-5684-9420.
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Saccharomyces cerevisiae"
                     /strain="X2180-1A"
                     /db_xref="taxon:4932"
     gene            4..2613
                     /gene="PLC1"
     CDS             4..2613
                     /gene="PLC1"
                     /EC_number="3.1.4.11"
                     /codon_start=1
                     /product="phospholipase C"
                     /db_xref="PID:d1002725"
                     /db_xref="PID:g287612"
                     Translation="MTESAIDDQRFNLTKELQRHSCRDQGKITQKDDALDFISYSSFQ
                     SSFNTDQKSANNGSTVRRSIRSIFRRAAELPRVHMGPLTYSHGINELVNKKLRKDCDL
                     STLCRVLQRGIRMIRMTRRRRKFYEFKLINNNGQIIWKDGSKYLELDSVKDIRIGDTA
                     STYQEEVDPKRLRSDSKLWIAIIYKVSNKLKALHVVALNELDFNTFLSCICGLVKLRR
                     ELMESILLPDNSQFARIHWQITVSEKEEDEKKDTLSFADVKKLCDKFHIYVSTGQLLE
                     FFQLADINHNGLLNYFEFEKFIKILKNRKEVNMIWSKFTKPPHSHLSFENFFQFLITE
                     QHEQVDRQTAWSYFIKYREPTQLTMGQDGFTKFLKEQPYLVEVKEELYSKPLNHYFIA
                     SSHNTYLLGKQIAETPSVEGYIQVLQQGCRCVEIDIWDGENGPVVCHGFLTSAIPLKT
                     VIRVIKKYAFITSPYPLIISLEINCNKDNQKLASLIMREVLAEQLYFVGTRTDKLPSP
                     RELKHKILLKSKKTSEATRGLSVNEPFPSSFSSSYESANEQELRMKDDSTNSSSATNS
                     SSMQRIKRIGLKKHADIINDVSNISGIHGIKFRNFSLPESKTIAHCFSLNERKVEYMI
                     KDKHLKLSLDKHNRRYLMRVYPHVLRYKSSNFNPIPFWKAGVQMVATNWQTNDIGQQL
                     NLAMFQILDHQPDGSFKSGYVLKPKKLLPVVTKAKMIPLIYEHFENGSDPVTVKIRIL
                     STQLLPRLNDTSPSRNNTNSFVKVEFHTDDEPTMPISIDKGTRISATEASTKSSQGNG
                     FNPIWDAEVSITLKDTDLTFIKFMVISEETQIASVCLKLNYLRMGYRHIPLFNMEGEQ
                     YIFCTLFIHTQIL"
BASE COUNT      930 a    426 c    519 g    768 t
ORIGIN
        1 tgaatgactg aaagtgctat agatgaccaa aggtttaatc ttacaaagga gttacaacga
       61 cactcatgca gagatcaagg gaaaataaca caaaaggatg atgctttaga ttttataagt
      121 tacagttctt ttcaatcctc ttttaatact gaccaaaaaa gcgcgaacaa tggaagtact
      181 gtgagaagaa gcataaggtc gatttttaga agagcagccg aattgcctag agtccatatg
      241 gggcctctta cttattcaca tgggataaat gagcttgtta acaagaaatt aagaaaagac
      301 tgtgatctca gcacgctatg tcgcgtattg caaagaggaa tcaggatgat taggatgaca
      361 agaagaagaa ggaagttcta tgaatttaaa ttaatcaata acaacgggca aataatatgg
      421 aaagatggtt cgaagtatct ggaattagac tccgttaaag acatcagaat cggtgatacg
      481 gcgagtactt atcaagagga agtagatccg aaaagattga gatcggacag taaactttgg
      541 attgctatta tttataaggt ttccaacaaa ttgaaagcgt tacacgtagt tgctttgaat
      601 gaattggact ttaacacatt cttaagctgc atttgcggtt tagtgaagtt aaggagggaa
      661 ttaatggaaa gcatactttt gcctgataat tcgcaattcg ctaggataca ttggcaaatt
      721 actgtttctg aaaaagagga agatgagaaa aaggacacat tgagctttgc agatgtgaag
      781 aaactatgtg ataaatttca tatttatgtg tcaactggtc aattgttaga gtttttccaa
      841 ttagcagaca tcaatcataa cggactatta aattattttg aatttgaaaa gttcattaaa
      901 atcttaaaga ataggaaaga ggtaaatatg atttggtcta aatttacaaa acctccacac
      961 tcccacttat catttgaaaa tttttttcaa tttttaatta cagagcagca tgagcaagtg
     1021 gatcgtcaaa ctgcatggtc atattttatt aagtacagag aaccgactca gctgacgatg
     1081 gggcaagatg gttttaccaa gttcttgaag gagcaaccat atttggtgga ggttaaagag
     1141 gagttatatt caaagccatt aaaccattat tttattgcat cttcacataa tacttattta
     1201 ttggggaaac aaattgcaga aactccatct gtggagggat atattcaagt cctacaacaa
     1261 ggttgtaggt gcgtagaaat tgatatatgg gatggtgaaa atgggccagt ggtatgccat
     1321 ggatttttaa cgtcagctat tcccttaaaa acagttatac gcgtgataaa gaagtacgct
     1381 tttataacgt ctccgtatcc tttgattatt tcactggaaa taaactgtaa caaggataat
     1441 caaaaattgg caagcctgat aatgagagag gttttagcag aacagctgta ctttgttggc
     1501 acaagaactg ataaactgcc atcaccaagg gaattaaaac ataaaatact gttgaaatcc
     1561 aagaagacga gtgaagcaac tagaggtctt agcgttaatg agccatttcc atcttctttt
     1621 agttcttctt acgaatcagc taatgaacag gaattaagaa tgaaagatga ctccacgaac
     1681 agtagtagcg caacaaattc ttctagtatg caacgcatca aaagaattgg attaaaaaag
     1741 cacgctgata taattaatga tgtttctaac atttctggaa tacatggcat caagttcagg
     1801 aatttctctt taccagagtc caaaacgata gcgcattgtt tttcactgaa tgagcgcaaa
     1861 gtagaataca tgataaaaga caaacactta aaattatcgt tggataaaca taatagaaga
     1921 tatttgatga gagtctatcc tcacgtttta cgatacaaat catcaaattt taacccaata
     1981 cctttttgga aggccggtgt acaaatggtg gctacgaatt ggcaaactaa tgatattgga
     2041 cagcaactta atctggcaat gtttcaaata ttagatcacc aacctgatgg aagttttaaa
     2101 tcaggatacg tgctaaaacc aaaaaagttg ctacctgtag taacaaaggc gaaaatgatt
     2161 ccattaattt acgaacattt tgaaaatggt agtgatcctg ttactgttaa aattcgaatt
     2221 ttatctacac agttattgcc cagattgaat gatacatcgc caagcagaaa taacaccaat
     2281 tcctttgtta aagtggagtt tcataccgat gacgagccga caatgcccat ctcaatcgat
     2341 aagggtacaa ggatttctgc cactgaagca tccactaaga gttctcaagg gaatggattc
     2401 aacccaatat gggatgctga agtttctatc acattgaaag atacagacct cacatttata
     2461 aaattcatgg ttatctctga agagacccag attgcatctg tttgccttaa gctgaactat
     2521 ttgaggatgg gatatcgtca cattccgttg tttaacatgg agggggaaca atacatattt
     2581 tgcactttat ttattcatac acaaatttta taggcgatct ttttccggcc aaatacacat
     2641 att