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LOCUS       YSCPRKINAS   2970 bp    DNA             PLN       08-MAR-1996
DEFINITION  Saccharomyces cerevisiae protein kinase (RPK1) gene, complete cds.
ACCESSION   L08909
NID         g1197057
KEYWORDS    RPK1 gene; protein kinase.
SOURCE      Saccharomyces cerevisiae (strain FL100) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota;
            Hemiascomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Poch,O., Schwob,E., de Fraipont,F., Camasses,A., Bordonne,R. and
            Martin,R.P.
  TITLE     RPK1, an essential yeast protein kinase involved in the regulation
            of the onset of mitosis, shows homology to mammalian
            dual-specificity kinases
  JOURNAL   Mol. Gen. Genet. 243 (6), 641-653 (1994)
  MEDLINE   94301294
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Saccharomyces cerevisiae"
                     /strain="FL100"
                     /db_xref="taxon:4932"
     gene            304..2896
                     /gene="RPK1"
     TATA_signal     304..310
                     /gene="RPK1"
                     /note="putative"
     mRNA            322..2896
                     /gene="RPK1"
     CDS             454..2748
                     /gene="RPK1"
                     /codon_start=1
                     /product="protein kinase"
                     /db_xref="PID:g1197058"
                     /translation="MSTNSFHDYVDLKSRTNTRQFSDDEEFTTPPKLSNFGSALLSHT
                     EKTSASEILSSHNNDKIANRLEEMDRSSSRSHPPPSMGNLTSGHTSTSSHSTLFGRYL
                     RNNHQTSMTTMNTSDIEINVGNSLDKSFERIRNLRQNMKEDITSKYAERRSKRFLISN
                     RTTKLGPAKRAMTLTNIFDEDVPNSPNQPINARETVELPLEDSHQTNFKETKRNTDYD
                     SIDFGDLNPIQYIKKHNLPTSDLPLISQIYFDKQREENRQAALRKHSSRELLYKSRSS
                     SSSLSSNNLLANKDNSITSNNGSQPRRKVSTGSSSSKSSIEIRRALKENIDTSNNSNF
                     NSPIHKIYKGISRNKDSDSEKREVLRNISINANHADNLLQQENKRLKRSLDDAITNEN
                     INSKNLEVFYHRPAPKPPVTKKVEIVEPAKSASLSNNRNIITVNDSQYEKIELLGRGG
                     SSRVYKVKGSGNRVYALKRVSFDAFDDSSIDGFKGEIELLEKLKDQKRVIQLLDYEMG
                     DGLLYLIMECGDHDLSQILNQRSGMPLDFNFVRFYTKEMLLCIKVVHDAGIVHSDLKP
                     ANFVLVKGILKIIDFGIANAVPEHTVNIYRETQIGTPNYMAPEALVAMNYTQNSENQH
                     EGNKWKVGRPSDMWSCGCIIYQMIYGKPPYGSFQGQNRLLAIMNPDVKIPFPEHTSNN
                     EKIPKSAIELMKACLYRNPDKRWTVDKVLSSTFLQPFMISGSIMEDLIRNAVRYGSEK
                     PHISQDDLNDVVDTVLRKFADYKI"
     misc_signal     2801..2873
                     /gene="RPK1"
                     /note="transcription/termination signal"
BASE COUNT     1105 a    512 c    541 g    812 t
ORIGIN
        1 aacatttcaa aaataaacga caaaaacact gaatacaccc taaaagagta aagaacatta
       61 aagtttttgt ttgagcatta ctgtgatgcc acaatttata tatcatacat gtttatatat
      121 tctgaccaat acctttattt atggtgtgca gtgacacaaa ataatttttg gtgtcacttc
      181 catcgtatta aatgataata tcaattagaa atagtaaaca aaaccaaaaa aaactaccaa
      241 gggttgcaaa gagtaatagt gatgtttatg tgaaggcaag cttaaagaga atttaagatc
      301 tactatatta atagtaacct acaaaattta tagaagccta cttgacccag cccactgaag
      361 tctgaatagg aatattgcag ttgtttatag aagtagcttt acaaccaaaa taataaattg
      421 tttctgcaag aactggatga tctcaaaaca aacatgtcaa caaactcatt ccatgattat
      481 gtggatttaa aatcgagaac taatacacga cagttttcag atgacgaaga gttcactacg
      541 cctccaaaac taagcaattt cggatcagct ttactttccc acacagaaaa aacttcagca
      601 tcagagatat tatcaagtca taataatgac aagatcgcaa atcgattaga agaaatggac
      661 aggagttcat caaggagtca ccctccaccg tcaatgggca atttgacatc cggtcatact
      721 agtacctcat cgcattcaac cttgttcgga cgatatctga gaaataatca ccagactagc
      781 atgacgacga tgaacactag tgacatagag ataaatgttg gaaatagtct tgataagagt
      841 tttgaaagga taaggaattt gcgacaaaat atgaaagaag atattacgtc aaagtatgct
      901 gaaaggagaa gtaagagatt tttaatatcc aataggacaa cgaagctggg tcctgcaaag
      961 agagcgatga ctttgacaaa tatctttgat gaggatgtgc ctaactctcc aaaccagcca
     1021 ataaatgcaa gggagacagt ggaattacca cttgaggatt ctcaccaaac aaactttaaa
     1081 gagacgaaga gaaatacgga ttatgattca attgattttg gagatttgaa tcctatccag
     1141 tatattaaaa aacataatct tcccacaagt gaccttccac taatatctca aatctacttt
     1201 gataaacaaa gagaagaaaa tagacaagca gcactccgaa aacatagttc cagagaattg
     1261 ctttataaaa gtaggtcttc ttcctcttca ctttctagta acaacttatt ggcaaacaag
     1321 gacaattcta taacatccaa taatggttct cagcccaggc gaaaagtttc taccggatca
     1381 tcttcatcta agtcatcgat cgaaataaga agagctctca aggagaatat tgatactagc
     1441 aataacagca atttcaacag cccaattcat aaaatttata aaggaatttc cagaaataaa
     1501 gattccgact ccgaaaaaag agaagtactg cgaaacataa gcataaacgc aaatcacgct
     1561 gataatctcc ttcaacaaga gaataagaga ctaaaacgat cattggatga tgcaataacg
     1621 aatgagaata taaacagtaa aaatctagaa gtattttacc atcgacctgc tcccaaacct
     1681 ccagtcacca agaaagttga aattgttgaa cctgcaaagt ccgcttcttt atcgaataat
     1741 agaaatataa ttacagtaaa tgactcccag tacgaaaaaa tagaactttt gggtagaggt
     1801 ggatcctcca gagtttacaa ggtgaaagga tctggcaata gggtatacgc gctcaaaagg
     1861 gtgtcttttg acgcttttga cgattcaagt attgatggat tcaaaggaga aatagaactt
     1921 ctggaaaaat tgaaagacca aaagcgtgta atccaactac tagattatga aatgggggat
     1981 ggtttattgt atttgataat ggaatgtggt gatcatgatt tgtcacaaat ccttaaccaa
     2041 agaagcggca tgccactgga ttttaatttt gttagattct atacaaagga aatgttgctg
     2101 tgcattaagg tagttcatga tgcgggcata gttcattcgg atttaaaacc tgcaaatttt
     2161 gttttagtga aaggtatctt aaaaatcatt gattttggta tagcaaacgc ggtaccggaa
     2221 catacggtga atatatatcg tgaaactcaa attgggactc caaattatat ggcaccagaa
     2281 gcactagttg ctatgaatta cacacaaaat agtgagaacc aacatgaggg aaacaagtgg
     2341 aaagtgggga gaccatctga tatgtggtca tgcggttgta ttatatatca gatgatttac
     2401 gggaaacccc catatggcag tttccaaggc caaaataggc tgttggctat tatgaatcct
     2461 gatgtgaaaa tcccatttcc tgaacatact agcaataatg aaaagattcc aaagtctgcc
     2521 attgaattaa tgaaagcatg tctgtacagg aacccagaca aaagatggac tgtggataaa
     2581 gtcctgagta gcactttcct tcaacctttt atgatatccg gatcgatcat ggaagacctt
     2641 attaggaatg ccgttagata tggctctgag aagcctcata tatcacaaga tgatctcaat
     2701 gatgtggtag acactgtttt aaggaaattt gcagattaca aaatttagaa gagccgcata
     2761 aggaagaaaa gcatgtgcca gttatgaaca taaatacatt tagagtttgc catgcatgta
     2821 ctgatttatt tactttgata aactcttttt tacatattta tatatattca tttaaataaa
     2881 tcatcaatca atttttcgac acagacactg aatacttgaa ataccaagaa aaggataact
     2941 atcctctaac ggcgctcagt gaaatattat
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