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LOCUS       HUMACTSM1     976 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 1 & ivs flanks.
ACCESSION   K01741
NID         g178059
KEYWORDS    actin.
SEGMENT     1 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
COMMENT     [1] the amino acid sequences deduced from these nucleotide
            sequences are identical to those reported for bovine aortic smooth
            muscle actin (Vandekerckhove,J., and Weber,K. (1979) 14, 123-133),
            except for codon 309, in which codon gcn for alanine is replaced by
            codon ftc for valine.
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     exon            <200..328
                     /gene="ACTA2"
                     /note="actin prepeptide; G00-125-197"
     intron          329..>976
                     /gene="ACTA2"
                     /note="actin intron 1"
BASE COUNT      295 a    205 c    193 g    283 t
ORIGIN      1 base upstream of HindIII site.
        1 aagcttatag tgtaactaaa gtacttccag atacatgcta tattagataa gggtcagagg
       61 atagcactag gacacagagg agggccccca aacccagcct tggacgtggg agttgtcagg
      121 taagtgccac aaatgcccaa ttacagctga ggctgcttcc tccctgtttt ctatagaatc
      181 ctgtgaagca gctccagcta tgtgtgaaga agaggacagc actgccttgg tgtgtgacaa
      241 tggctctggg ctctgtaagg ccggctttgc tggggacgat gctcccaggg ctgttttccc
      301 atccattgtg ggacgtccca gacatcaggt gaggaaaatt atgattggaa cataacagga
      361 cacagattag gttcctctgt gttcatgttt atccccagat tgtctatctc tttctgccca
      421 gaagttatgt aaccttgtta caaatagacc tctaatgatt aaggtccagg agtaaatgcc
      481 aattcacata taatgtagac agattatctg atgggcatct atcagataca aagtctgccc
      541 ctttttcatg tcctttttgt ctaaatatag tcattatcat catcatcatc atcatcaaat
      601 catttcatca ccatcagaaa tgcttataca ttatcctgat gtataccaaa gctactgttt
      661 ggaaagaaac taaaataaaa gtccaggtca cttaaccata cagggctgat gttagatgaa
      721 agcaagcatc gataccaaat gcaattttac ataatattac ctgtcaacaa aatatatttg
      781 gacagccgca tggtaatttt acacattatg tgtaaacaaa gtattggtgg catcacatgg
      841 taaaaactca gtaatttcac ctcagaaatt ctcctccaca ccagaaatgt agtttgtgca
      901 ttgaggctat ctgattgatg tttatgcctc tctgcttggg atatattcat gagaataaat
      961 aatagaaacc tctccc
//
LOCUS       HUMACTSM2     292 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 2 and ivs flanks.
ACCESSION   K01742
NID         g178060
KEYWORDS    actin.
SEGMENT     2 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     intron          <1..149
                     /gene="ACTA2"
                     /note="actin intron 1"
     exon            150..278
                     /gene="ACTA2"
                     /note="G00-125-197"
                     /number=2
     intron          279..>292
                     /gene="ACTA2"
                     /note="actin intron 2"
BASE COUNT       88 a     54 c     78 g     72 t
ORIGIN      45 bases upstream of MboII site.
        1 catagtggca atgagactca atatgtttat tgttgctgat aaccagaaga ggaacttccc
       61 atctcaatca cttaaccttt tgtggcttgg ctgtaactga ttcacaagtt ttgggagatg
      121 ctgactcata atgtgttgaa cctttttagg gggtgatggt gggaatggga caaaaagaca
      181 gctacgtggg tcacgaagca cagagcaaaa gaggaatcct gaccctgaag tacccgatag
      241 aacatggcat catcaccaac tgggacgaca tggaaaaggt atgggaggtt gc
//
LOCUS       HUMACTSM3     223 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 3 and ivs flanks.
ACCESSION   K01743
NID         g178061
KEYWORDS    actin.
SEGMENT     3 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     intron          <1..43
                     /gene="ACTA2"
                     /note="actin intron 2"
     exon            44..154
                     /gene="ACTA2"
                     /note="G00-125-197"
                     /number=3
     intron          155..>223
                     /gene="ACTA2"
                     /note="actin intron 3"
BASE COUNT       57 a     70 c     48 g     48 t
ORIGIN      42 bases upstream of BglII site.
        1 acagcacatc attgtgtttc tcctctgtcc cccaactcac tagatctggc accactcttt
       61 ctacaatgag cttcgtgttg cccctgaaga gcatcccacc ctgctcacgg aggcacccct
      121 gaaccccaag gccaaccggg agaaaatgac tcaagtaagt atgctacccc agcacacaac
      181 actgtggggt ctggggaaac ttctgttaga aggaggctat gca
//
LOCUS       HUMACTSM4     203 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 4 and ivs flanks.
ACCESSION   K01744
NID         g178062
KEYWORDS    actin.
SEGMENT     4 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     intron          <1..74
                     /gene="ACTA2"
                     /note="actin intron 3"
     exon            75..159
                     /gene="ACTA2"
                     /note="G00-125-197"
                     /number=4
     intron          160..>203
                     /gene="ACTA2"
                     /note="actin intron 5"
BASE COUNT       35 a     53 c     56 g     59 t
ORIGIN      50 bases upstream of the DdeI site.
        1 ccaggtgtgc tctcctgacc attctttgtc agatgggcac cttcactaac tcagctcttt
       61 ggattgcttt gcagattatg tttgagactt tcaatgtccc agccatgtat gtggctatcc
      121 aggcggtgct gtctctctat gcctctggac gcacaactgg taggtggctg ggcgggacag
      181 caagctggtt ggagcgcact gcc
//
LOCUS       HUMACTSM5     530 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 5 and ivs flanks.
ACCESSION   K01745
NID         g178063
KEYWORDS    actin.
SEGMENT     5 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     intron          <1..313
                     /gene="ACTA2"
                     /note="actin intron 4"
     exon            314..475
                     /gene="ACTA2"
                     /note="G00-125-197"
                     /number=5
     intron          476..>530
                     /gene="ACTA2"
                     /note="actin intron 6"
BASE COUNT      106 a    153 c    113 g    158 t
ORIGIN      48 bases upstream of the BglII site.
        1 cggactggag ttgagttaga atggacacgg ctgaacccag ccctaaaaga tcttcttcta
       61 gtgttcaaaa taagcccttg atctcatatt tctagcagaa cttttttcta ctgttttcct
      121 cttcctgttc ttttactttt tttcatcttc cccgagccct gtcccacctc acctctccta
      181 tcacatgccc tgttttctct ttttaaataa gaggctcgga aattccctct cccccagggc
      241 ttccctctac ttgtccagca agtaaccaca tgccagctgc catggtgact tatcacgccc
      301 aattgtctcc caggcatcgt gctggactct ggagatggtg tcacccacaa tgtccccatc
      361 tatgagggct atgccttgcc ccatgccatc atgcgtctgg atctggctgg ccgagatctc
      421 actgactacc tcatgaagat cctgactgag cgtggctatt ccttcgttac tactggtgag
      481 gcctgtggga gataaggggg agaggggagc agtgtgttgc tcagtacctc
//
LOCUS       HUMACTSM6     269 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 6 and ivs flanks.
ACCESSION   K01746
NID         g178064
KEYWORDS    actin.
SEGMENT     6 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     intron          <1..42
                     /gene="ACTA2"
                     /note="actin intron 5"
     exon            43..234
                     /gene="ACTA2"
                     /note="G00-125-197"
                     /number=6
     intron          235..>269
                     /gene="ACTA2"
                     /note="actin intron 6"
BASE COUNT       65 a     65 c     72 g     67 t
ORIGIN      43 bases upstream of the DdeI site.
        1 cagaccctaa tgtttgccac aatgtttgta tctggttgac agctgagcgt gagattgtcc
       61 gggacatcaa ggagaaactg tgttatgtag ctctggactt tgaaaatgag atggccactg
      121 ccgcatggtc atcctccctt gagaagagtt acgagttgcc tgatgggcaa gtgatcacca
      181 tcggaaatga acgtttccgc tgcccagaga ccctgttcca gccatccttc atcggtaggc
      241 aacaaacgcc cttttcctgg gaaggggag
//
LOCUS       HUMACTSM7     408 bp    DNA             PRI       30-OCT-1994
DEFINITION  Human smooth muscle actin gene (aortic): exon 7 and ivs flanks.
ACCESSION   K01747
NID         g178065
KEYWORDS    actin.
SEGMENT     7 of 7
SOURCE      Human DNA, clone lambda-Ha201.
  ORGANISM  Homo sapiens
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Ueyama,H., Hamada,H., Battula,N. and Kakunaga,T.
  TITLE     Structure of a human smooth muscle actin gene (aortic type) with a
            unique intron site
  JOURNAL   Mol. Cell. Biol. 4 (6), 1073-1078 (1984)
  MEDLINE   84245506
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="10q22-q24"
     gene            join(K01741:200..976,K01742:1..292,K01743:1..223,
                     K01744:1..203,K01745:1..530,K01746:1..269,1..279)
                     /gene="ACTA2"
     CDS             join(K01741:200..328,K01742:150..278,K01743:44..154,
                     K01744:75..159,K01745:314..475,K01746:43..234,98..279)
                     /partial
                     /gene="ACTA2"
                     /note="actin prepeptide"
                     /codon_start=1
                     /db_xref="GDB:G00-125-197"
                     /db_xref="PID:g178067"
                     /translation="MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQG
                     VMVGMGQKDSYVGHEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEE
                     HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG
                     VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKL
                     CYVALDFENEMATAAWSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGI
                     HETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIVDRMQKEITALAPSTMKIK"
     exon            98..279
                     /gene="ACTA2"
                     /note="actin prepeptide (exon 6); putative"
     exon            98..>279
                     /gene="ACTA2"
                     /note="actin prepeptide; G00-125-197"
                     /number=6
     intron          280..>408
                     /note="intron 7"
BASE COUNT      110 a    102 c     97 g     99 t
ORIGIN      43 bases upstream of the DdeI site.
        1 cactgcgaag atcatccaga tgttcaagtc tggtcttcca tgtctagcct aggttttact
       61 ctccacttaa ggaccatggc ctgtgtctct tttccaggga tggagtctgc tggcatccat
      121 gaaaccacct acaacagcat catgaagtgt gatattgaca tcaggaagga cctctatgct
      181 aacaatgtcc tatcaggggg caccactatg taccctggca ttgtcgaccg aatgcagaag
      241 gagatcacgg ccctagcacc cagcaccatg aagatcaagg tactgttcag tgacagaccc
      301 agtggcaatg ccgccagcag tgtcagccca taaccacaga taagaactct tcagagactg
      361 tattttggtg acatgcaatt gtgggtttta ggggtaggta atgacaca
//
 
 
 
 

        the above report in   format