LOCUS       SCU34636     2079 bp    DNA             PLN       16-AUG-1996
DEFINITION  Saccharomyces cerevisiae cytochrome P450 gene, complete cds.
ACCESSION   U34636
NID         g1493800
KEYWORDS    .
SOURCE      baker's yeast.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota;
            Hemiascomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Skaggs,B.A., Alexander,J.F., Pierson,C.A., Schweitzer,K.S.,
            Chun,K.T., Koegel,C., Barbuch,R. and Bard,M.
  TITLE     Cloning and characterization of the Saccharomyces cerevisiae C-22
            sterol desaturase gene, encoding a second cytochrome P-450 involved
            in ergosterol biosynthesis
  JOURNAL   Gene 169 (1), 105-109 (1996)
  MEDLINE   96186912
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Skaggs,B.A., Alexander,J.F., Pierson,C.A., Schweitzer,K.S.,
            Chun,K.T., Koegel,C., Barbuch,R. and Bard,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-1995) Beth Skaggs, Biology, Indiana University-
            Purdue University at Indianapolis, 723 West Michigan Street,
            Indianapolis, IN 46202, USA
COMMENT     On Aug 16, 1996 this sequence version replaced gi:1235990.
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Saccharomyces cerevisiae"
                     /db_xref="taxon:4932"
                     /chromosome="XIII"
     CDS             376..1992
                     /function="C-22 sterol desaturase"
                     /codon_start=1
                     /product="cytochrome P450"
                     /db_xref="PID:g1235991"
                     /translation="MSSVAENIIQHATHNSTLHQLAKDQPSVGVTTAFSILDTLKSMS
                     YLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWPIIGPFLESLDPKFEEYKAKWAS
                     GPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT
                     DYRKSLNGLFTKQALAQYLPSLEQIMDKYMDKFVRLSKENNYEPQVFFHEMREILCAL
                     SLNSFCGNYITEDQVRKIADDYYLVTAALELVNFPIIIPYTKTWYGKKTADMAMKIFE
                     NCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDDSRIYHREFTNKEISEAVFTFL
                     FASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMV
                     IKETLRYRPPVLMVPYVVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIP
                     ERWVEGSKASEAKKNWLVFGCGPHVCLGQTYVMITFAALLGKFALYTDFHHTVTPLSE
                     KIKVFATIFPKDDLLLTFKKRDPITGEVFE"
BASE COUNT      616 a    391 c    383 g    689 t
ORIGIN
        1 ccctcgttta agtctgcgaa gtctcgtacc ttttatcgct gctgacaaaa catagcccaa
       61 acctgcgtct atatgctgcc ggtgtttata tcgctattga agagagctca tgtttcggtt
      121 ttgaaaagat tttttatcga atcggttgta aaagatcgtc cttattgtct tcaggcaaag
      181 ggactgattg gtttatatac gcaagcaggg aatcttgttg gaagaaaaaa attatatata
      241 agctaattgt ctattgttcc aaatatctta ttcttactca tttctttctt gttatattgt
      301 ttccttaatt tttatcacaa taaacaaaac atcacatttt gctattccaa tagacaataa
      361 atacctttta acaaaatgag ttctgtcgca gaaaatataa tacaacatgc cactcataat
      421 tctacgctac accaattggc taaagaccag ccctctgtag gcgtcactac tgccttcagt
      481 atcctggata cacttaagtc tatgtcatat ttgaaaatat ttgctacttt aatctgtatt
      541 cttttggttt gggaccaagt tgcatatcaa atcaagaaag gttccatcgc aggtccaaag
      601 tttaagttct ggcccatcat cggtccattt ttggaatcct tagatccaaa gtttgaagaa
      661 tataaggcta agtgggcatc cggtccactt tcatgtgttt ctattttcca taaatttgtt
      721 gttatcgcat ctactagaga cttggcaaga aagatcttgc aatcttccaa attcgtcaaa
      781 ccttgcgttg tcgatgttgc tgtgaagatc ttaagacctt gcaattgggt ttttttggac
      841 ggtaaagctc atactgatta cagaaaatca ttaaacggtc ttttcactaa acaagctttg
      901 gctcaatact taccttcatt ggaacaaatc atggataagt acatggataa gtttgttcgt
      961 ttatctaagg agaataacta cgagccccag gtctttttcc atgaaatgag agaaattctt
     1021 tgcgccttat cattgaactc tttctgtggt aactatatta ccgaagatca agtcagaaag
     1081 attgctgatg attactattt ggttacagca gcattggaat tagtcaactt cccaattatt
     1141 atcccttaca ctaaaacatg gtatggtaag aaaactgcag acatggccat gaagattttc
     1201 gaaaactgtg ctcaaatggc taaggatcat attgctgcag gtggtaagcc agtttgtgtt
     1261 atggatgctt ggtgtaagtt gatgcacgat gcaaagaata gtaacgatga tgattctaga
     1321 atctaccaca gagagtttac taacaaggaa atctccgaag ctgttttcac tttcttattt
     1381 gcttctcaag atgcctcttc ttctttagct tgttggttgt tccaaattgt tgctgaccgt
     1441 ccagatgtct tagctaagat cagagaagaa caattggctg ttcgtaacaa tgacatgtct
     1501 accgaattga acttggattt gattgagaaa atgaagtaca ccaatatggt cataaaagaa
     1561 actttgcgtt acagacctcc tgtcttgatg gttccatatg ttgttaagaa gaatttccca
     1621 gtttccccta actataccgc accaaagggc gctatgttaa ttccaacctt atacccagct
     1681 ttacatgatc ctgaagttta cgaaaatcct gatgagttca tccctgaaag atgggtagaa
     1741 ggctctaagg ctagtgaagc aaagaagaat tggttggttt ttggttgtgg tccacacgtt
     1801 tgcttaggtc aaacatatgt catgattacc ttcgccgctt tgttgggtaa atttgcacta
     1861 tatactgatt tccatcatac agtgactcca ttaagtgaaa aaatcaaggt tttcgctaca
     1921 attttcccaa aagatgattt gttactgact ttcaaaaaga gagacccaat tactggagaa
     1981 gtcttcgaat aatggggaaa aacagaactt tgtccagaca ataaatcata tttacttcat
     2041 taagggttgg cgttttgaat acttttattt tcttccctt