LOCUS YSCRAD25A 3955
bp DNA
PLN 09-MAY-1993
DEFINITION Saccharomyces cerevisiae DNA helicase (RAD25) gene,
complete cds.
ACCESSION L01414
NID g172326
KEYWORDS DNA helicase; RAD25 gene; homologue.
SOURCE Saccharomyces cerevisiae (library:
EMBL3 lambda) DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota;
Hemiascomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 3955)
AUTHORS Park,E.K., Guzder,S.N., Weeda,G., Hoeijmakers,J.H.J.,
Prakash,S.,
Prakash,L., Koken,M.H.N. and Jaspers-Dekker,I.
TITLE RAD25 (SSL2), the yeast homolog
of the human xeroderma pigmentosum
group B DNA repair gene, is essential for viability
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 89, 11416-11420
(1992)
MEDLINE 93087538
FEATURES
Location/Qualifiers
source
1..3955
/organism="Saccharomyces cerevisiae"
/db_xref="taxon:4932"
/tissue_lib="EMBL3 lambda"
gene
720..3251
/gene="RAD25"
CDS
720..3251
/gene="RAD25"
/note="domain I (Walker type A nucleotide binding domain),
aa 383..397; domain I-a, aa 405..425; domain II, aa
479..493; domain III, aa 511..529; domain IV, aa 623..639;
domain V, aa 645..671; domain VI, aa 679..695; homologous
to human ERCC3 gene product; putative"
/codon_start=1
/product="DNA helicase"
/db_xref="PID:g172327"
/translation="MTDVEGYQSKSKGKIFPDMGESFFSSDEDSPATDAEIDENYDDN
RETLEGRGERDTGAMVTGLKKPRKKTKSSRHTAADSSMNQMDAKDKALLQDTNSDIPA
DFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLAEQAQDFLVTI
AEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRLSKVPVAESIINFIKGATI
SYGKVKLVIKHNRYFVETTQADILQMLLNDSVIGPLRIDSDHQVQPPEDVLQQQLQQT
AGKPATNVNPNDVEAVFSAVIGGDNEREEEDDDIDAVHSFEIANESVEVVKKRCQEID
YPVLEEYDFRNDHRNPDLDIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGK
TLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTES
GLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAH
AKLGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFY
QEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSDNVYALQEYALKMGK
PFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQ
EAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLH
GMENIPNLAYASPRERRELLQEVLLKNEEAAGIEVGDDADNSVGRGSNGHKRFKSKAV
RGEGSLSGLAGGEDMAYMEYSTNKNKELKEHHPLIRKMYYKNLKK"
BASE COUNT 1197 a 754 c
806 g 1198 t
ORIGIN
1 agatcttcac ggaacatggt
cttatcatga gagttgtcaa cagccgcttt cgctaacgca
61 tccaatgcgg ctgctgaagc tagtcggtaa
ccttcaataa tagtttgagg gtggattttg
121 gattgatcaa tcaacttctc tgcttctcta
agcaactctg cgctcaaaac agtaacacta
181 gtggtaccgt ctccaacttc gtcatcctgc
accttactga tgttcaccaa cactttggct
241 gccggattat ccagcggaat tgacttcaga
attgtagtca ccatcgtttg ttaccatgca
301 agtgttactg gacgcacttt gcaataattt
atccatccct tttgggccta atgtgctttt
361 caccaaatca cccacggcaa tggcgccgac
aaacgccgac aaacgggcgt tttccgctct
421 ttcttctgta acctggtctc caaatatctg
tacactcatc ctgaatcttt tgagctttgt
481 acgactgact cttcttgcct atttaatggc
cacctttcat cctctttttt attgctctgc
541 tcttgtcttt taaactcgcc accgcgaaga
gaaaaaaatt tcagatcgtt agaaacgagg
601 tttttctttt aagaagtata aggaaaaagg
tccttagtaa ggataggtaa tagtaaggat
661 atctacatta tttagtaaaa gtagcatcta
ctggcgttgg aatcagtgga agataaatca
721 tgacggacgt tgaaggctac caatctaaga
gcaaggggaa aatctttcca gatatgggtg
781 agtctttttt ctcatcagat gaggattcac
cagctactga tgcagagatc gatgagaact
841 atgatgataa cagggaaacg ttagaaggac
gtggagaaag ggataccggc gccatggtaa
901 cagggttgaa gaagcctcgg aaaaagacca
agagtagcag gcatacagcg gcggattcct
961 ctatgaatca aatggacgct aaggataaag
cgcttttgca ggacactaat agcgatatac
1021 cagcagattt cgttccagat tccgtctctg gaatgttcag
aagccatgac tttagttatt
1081 tgcggttaag accagaccat gcttccaggc ccctttggat
ctcgccaagt gacggtagga
1141 tcattctgga gagtttctct ccattagcag aacaggctca
ggactttctg gtcaccattg
1201 cagagcctat aagtagaccc tcccatattc atgaatacaa
gatcacagca tactctttgt
1261 atgcagctgt ttctgtaggg ttggagacgg atgatatcat
ttcggttctg gatagattat
1321 ccaaagtacc cgttgctgaa tcgataatca acttcattaa
aggggctacc atttcatacg
1381 gtaaagtgaa actagttatt aagcataata gatatttcgt
ggagactaca caagcagata
1441 ttctgcaaat gttgctgaat gattccgtca tcgggcccct
aagaatagat agcgatcatc
1501 aagtacagcc accagaggat gtattacaac aacaacttca
gcaaaccgct ggaaaacccg
1561 ctactaatgt taatccgaac gatgtggaag ccgtgtttag
tgcggttata ggtggtgata
1621 atgagcggga agaagaagat gatgatattg atgccgttca
ctcctttgaa attgccaatg
1681 agtctgttga agtcgtaaag aaaagatgtc aagaaatcga
ttatcccgtg ctggaagaat
1741 acgactttag aaacgaccat agaaatccgg atttagatat
tgatttgaaa ccttccactc
1801 aaatcagacc ctatcaagaa aaatcgctga gtaaaatgtt
tggtaatggg cgtgctcgtt
1861 cagggattat tgttttgcca tgtggcgcag gtaaaacttt
ggtcggtatt accgcagcat
1921 gtactataaa aaaatccgta attgttttat gtacttcatc
agtctccgtc atgcaatgga
1981 ggcagcaatt tttacaatgg tgtaccttac aacctgaaaa
ttgtgctgtt ttcacctctg
2041 ataataagga aatgttccag acagaatccg gtttggttgt
ttccacttat tcaatggtgg
2101 caaacacaag aaacaggtct catgattcac aaaaagttat
ggatttcttg accggtaggg
2161 aatgggggtt cattattctt gacgaagttc atgtggtgcc
cgccgcaatg ttccgtaggg
2221 tggtctccac catcgcagca cacgccaaat tgggactgac
cgcaacgctg gttagagaag
2281 atgataaaat tggtgatctg aattttttaa ttggtccaaa
actttatgaa gcaaattgga
2341 tggaattatc ccaaaaaggg catattgcaa atgtccaatg
tgcagaggtt tggtgtccca
2401 tgacagcaga attttaccaa gagtatttaa gagaaactgc
aaggaaaaga atgctactgt
2461 atatcatgaa tccaacaaag tttcaggcat gccaattctt
aattcaatat cacgaaagga
2521 ggggtgataa gatcatcgtt ttttcagata atgtttacgc
cttacaagaa tatgctttga
2581 aaatgggaaa accatttatt tatggttcca caccacaaca
agagcgtatg aacattctgc
2641 aaaatttcca gtacaatgac caaatcaata ccatatttct
atcaaaagtt ggtgatactt
2701 ccatcgattt accagaagct acctgtttaa ttcaaatatc
ttcgcactat gggtctcgtc
2761 gtcaagaagc ccaaagatta ggaagaattt taagagctaa
aagacgtaat gacgaaggtt
2821 tcaatgcgtt cttttattct ctggtttcta aggatacgca
agaaatgtac tactcaacaa
2881 agagacaagc gtttttggta gatcaaggtt acgcattcaa
agtcattaca catttacacg
2941 gaatggagaa cattccaaat ttggcatatg cttcacccag
agaacgtaga gagctattgc
3001 aagaagtatt attgaagaac gaagaagctg ctggtatcga
ggtcggtgat gacgctgaca
3061 attccgttgg gagaggttca aatggccaca aaagatttaa
atcaaaggcc gttagaggtg
3121 aaggttcatt gtctggtctt gctggcggtg aagatatggc
atatatggaa tacagtacca
3181 ataaaaacaa agaactgaag gaacatcatc cattaatcag
aaagatgtat tataagaatt
3241 tgaagaagtg aataggcttg gtttcattgt caccttccta
ttttgaatct attcagtcat
3301 aatacctttt tgttcccacc gtcttcttat tctttcgtcg
ctgttccttc ttttattgat
3361 tcgtcacgtc cattttcttc cataatataa aaaataaaaa
aaaaatagaa cattcctaat
3421 taaagcatcc aaatatactt acatatattt attttaaaaa
taactacata tcaatactta
3481 cttaaattgg tttggaatac ttgagaaggg gagtgagggt
ggaattgaac aaaatgcagg
3541 ttattaaaat gcggaaaggt ggggctgagc tttgctgtag
attgctcggg tattatatat
3601 catttatttt attaatttgt tacgttatgt tcagtttcaa
actccaaatt tcgtaactct
3661 tcaatgacgt ttcccaattc gttttcagag ttttccaatg
acgattgaat attaatttta
3721 aatttacttg ttatatcaat aacatgtatc acttgttggt
gtagcttgta tcgtttcctg
3781 tttaagtcaa cttttaattc ttccaatttt agttccgttg
aagttaccaa ttcctcagca
3841 tctgaaattt tagttttcat caagagattt gaatcattaa
tttttttttc cattttttca
3901 atctcgattc tttgcgctac taataatcgt tcgttctctt
gcttagagcg gatcc