How to Recycle QIAquick Purple Spin Columns

Adapted from: Siddappa et al., 2007, Biotechniques

1) When you are finished with the gel purification from Qiagen, place the used columns into the paired, used collection tubes.

2) Fill used column with 700 mL of 1 M HCl.

3) Store with cap closed in an airtight container for at least 24 hours (up to 30 days).

4) Once you have a bunch of columns and collection tubes in the 1 M HCl, to prepare a column for use:

Note: After 9 rounds of regeneration, columns have shown optimal performance in a series of assays (Siddappa et al. 2007).

1M HCl

FW HCl = 36.47; 37% HCl = 370 g/L » 10 M

Note: for HCl, 1 Molar = 1 Normal

Qiagen Buffer QBT (Equilibration Buffer)

© Copyright 2007 Department of Biology, Davidson College, Davidson, NC 28036
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