Adapted from: Siddappa et al., 2007, Biotechniques
1) When you are finished with the gel purification from Qiagen, place the used columns into the paired, used collection tubes.
2) Fill used column with 700 mL of 1 M HCl.
3) Store with cap closed in an airtight container for at least 24 hours (up to 30 days).
4) Once you have a bunch of columns and collection tubes in the 1 M HCl, to prepare a column for use:
Note: After 9 rounds of regeneration, columns have shown optimal performance in a series of assays (Siddappa et al. 2007).
FW HCl = 36.47; 37% HCl = 370 g/L » 10 M
Note: for HCl, 1 Molar = 1 Normal
Qiagen Buffer QBT (Equilibration Buffer)
[750 mM NaCl, 50 mM MOPS (pH 7.0),15% ethanol, 0.15% Triton X-100]
Dissolve 43.83g NaCl, 10.46g MOPS (free acid) in 800 mL dH2O. Adjust pH to 7.0. Add 150 mL 100% ethanol and 15 mL 10% triton X-100 solution. Adjust volume to 1 liter.
Store at room temperature.
© Copyright 2007 Department of Biology, Davidson College, Davidson, NC 28036
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