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The analysis portrayed by this figure further elaborated upon the patterns of functions of genes differentially expressed between PAH and PHH models. This was accomplished by multilateral pathway analysis (a approach which uses databases of molecular interaction to group individual genes with larger cellular pathways of a general known function) using the acquired RNA-seq data.
Darker blue squares (based on the scale in the upper right) indicate greater likelihood of statistical significance (lower p values) of change of transcript "enrichment," which can indicate a greater magnitude of either up- or down-regulation depending on the column heading.
Each of the five groups (I-V) corresponds largely to a specific manner by which differentially expressed genes were expressed and a family of gene function. Group I genes (all involved in muscle contraction and metabolism), for example, were largely down-regulated in PAH models – which may more directly explain some negative health consequences of PAH – and group III genes, tended to be down-regulated in PHH models and were all similarly implicated in autoimmunity, perhaps implying a means by which the rigor of this tissue type may be preserved.
Overall, this figure elaborates on reports of previous figures by distinguishing five fairly distinct patterns of gene expression that correlate with certain important categories of cellular functions implicated in the differentiation of PAH and PHH, thus providing a clearer link between gene alteration and changes in cell function, between PAH and PHH.References
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