Gene Networks Database
Heliocidaris erythrogramma Genes in Development:
Primary mesenchyme-specific genes
msp130 is a sceletogenic mesenchyme lineage-specific gene (Klueg et al., 1997).
The function of msp130 remains unclear, however it is evident
that it has been implicated in major primary mesenchyme cell functions (Parr et al., 1989).
There is also some experimental evidence that msp130
may play a role in the entrance of calcium ions into the
PMCs (Carson et al., 1985; Kabakoff et all., 1992).
msp130 is a single copy-gene in Heliocidaris (Klueg et al., 1995).
A probe made from a single msp130 exon from this species
hybridizes strongly to three transcripts of approximately
3.8, 3.0, and 1.8 kb.
Expression of the msp130 is not seen in blastulae
or during gastrulation. All three transcripts
are present after gastrulation, in larval stages.
Very low levels of the three msp130 transcripts
are detected at 36 and 48 hr (Klueg and Raff, unpublished data).
This is consistent with the observation that
only a small subset of mesenchyme cells express
msp130 protein during the early stages of sceletogenesis in
The levels of each of these transcripts are not
coincident throughout these later stages of development.
As development progresses, the relative level
of the 3.8 kb transcript increases, and the relative levels
of the 3.0- and 1.8-kb transcripts decrease.
The results of RNA blots suggest that the
3.0- and 1.8-kb transcripts are products of alternative splicing (Klueg et al., 1997).
In H. erythrogramma the msp130 protein is not expressed
in mesenchyme blastula or early gastrula stages.
It is first detected over 10 hr later in a small
subset in mesenchyme cells between late gastrula and
early larval stages and continues to be expressed
during adult rudiment formation (Parks et al., 1988).
This pattern of protein expression correlates
with the timing of the remnant larval and adult
sceleton secretion in H. erythrogramma (Parks et al., 1988;
Emlet, 1995). The delayed expression pattern
of msp130 is an example of a molecular heterochrony
associated with a the change in developmental mode (Klueg et al., 1997).
Method: RNA gel blot hybridization
Reference: Klueg et al., 1997
||16-hr early gastrula
||36 hr larva
||48 hr larva
- msp130 Heliocidaris tuberculata
- msp130 Eucidaris tribuloides
- msp130 Phyllacanthus parvispinus
- msp130 Asthenosoma ijimai
- msp130 Dendraster excentricus
- msp130 Mellita isometra
- msp130 Arabicia punctulata
- msp130 Strongylocentrotus droebachiensis
- msp130 Strongylocentrotus purpuratus
- msp130 Lytechinus variegatus
- msp130 Lytechinus pictus
UrchiNet Search the GeNet
Comments are welcome to Sveta Surkova
Copyright © 1997 GeNet Team