Gene Networks Database


Lytechinus variegatus Genes in Development: Primary mesenchyme-specific genes


Lvmsp130


Function

Lvmsp130 is a PMC marker gene. PMC-specific gene products participate in the synthesis of sceleton (Guss et al., 1997).
Msp130 is a sulfated cell-surface glycoprotein that has been implicated in calcium uptake or deposition (Carson et al., 1985; Leaf et al., 1987).

Protein

Subcellular location


Expression Pattern

Nothern blot analysis recognized the Lvmsp130 transcript of approximately 4kb, which is detectable by the hatched blastula stage. Expression levels peak at the mid gastrula stage and decrease through the late pluteus stage.
By whole-mount in situ hybridization, expression is detectable in the vegetal plate of hatched blastula-stage embryos, prior to PMC ingression. All PMCs in the embryo appear to express equal levels of the Lvmsp130 through the late gastrula stage. By the prism stage, however, a gradual reduction in transcript level begins in PMC throughout the embryo except those located in the ventrolateral clusters. By the pluteus larva stage, transcript levels are highest in cells at the tips of the elongating anterolateral and postoral rods, while intermediate levels of expression are visible in cells in the sheitel. At this stage the Lvmsp130 transcript is no longer detectable in cells distributed along the shafts of the sceletal rods (Guss et al., 1997).

mRNA level

Temporal accumulation (persent maximum abundance*)

Method 1: Nothern blot hybridization
Reference: Guss et al., 1997

Stage
Hatched blastula
Early mesenchyme blastula
Late mesenchyme blastula
Midgastrula
Late gastrula
Prism
Early pluteus
Late pluteus
Level
39
51
80
100
51
39
38
22

*Values were corrected for relative amounts of hybridizable RNA based on ubiquitin expression levels.

Spatial localization

Method 1: Whole-mount in situ hybridization
Reference: Guss et al., 1997

Stage
Hatched blastula (prior to PMCs ingression)
Late mesenchyme blastula
Late gastrula
Prism
Early pluteus
Late pluteus
Tissue
vegetal plate
all PMCs
all PMCs
PMCs in the ventrolateral clusters, particularly along the anonymous rods (highest level)
PMCs at the tips of the budding postoral arms (highest level)
PMCs at the tips of the postoral and anterolateral rods (highest level). Intermediate expression in the scheitel


Sequences


Regulatory Regions


Regulatory Connections

Upstream Genes

Lvmsp130

Downstream Genes


Evolutionary Homologues


Links


Bibliography


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Comments are welcome to Sveta Surkova
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