Gene Networks Database
pictus Genes in Development: Actins
LpC1 gene codes for the cytosceletal type actin (Fang et al., 1996). Actin represents one of the most abundunt and best characterized structural
components in eucaryotic cells, involved in a wide variety of cytoskeletal, locomotive, and tension-generating functions (Davidson, 1989).
Each haploid L. pictus genome contains a single copy of each of the actin genes LpC1, LpC2, LpC3, LpC4, and LpM.
Southern blot analysis of genomic DNA clones have detected no close linkage of L.pictus actin genes in contrast to
the cytosceletal actins of S. purpuratus, which are clustered into two linkage groups of related genes (Fang et al., 1996).
LpC1 protein belongs to a family of the cytoskeletal
actins (Fang et al., 1996).
LpC1 mRNA is the most abundant actin mRNA expressed
At the mesenchyme blastula stage the 2.1-kb LpC1 transcripts
become detectable in several cells at the center of the
During gastrulation, the LpC1 actin gene is
expressed exclusively in the cells located on the tip of
the elongating archenteron, the prospective secondary
mesenchyme cells (SMCs).
Later in gastrulation, these stained cells ingress from
the tip of the archenteron to form secondary mesenchyme. Some, maybe all, of them
migrate along the ectodermal wall, then penetrate and are retained along the
ectodermal epithelium; these cells are presumably the pigment-forming SMCs.
Endodermal cells, especially near the blastopore, begin to accumulate
the LpC1 mRNA during gastrulation. A few primary mesenchyme cells (PMCs) are heavily
stained in gastrulae. Some heavily stained secondary mesenchyme cells are
not embedded in the ectodermal wall, but are scattered in the blastocoel.
These cells probably belong to the subpopulation of secondary mesenchyme
cells, such as the blastopore masenchyme cells.
The LpC1 gene is subsequently expressed in the ectoderm
cells (ECs) at very low levels in late gastrula and thereafter.
Most of the large increase in prevalence of LpC1 RNA
between prism and pluteus stages is due to accumulation in
the differentiated gut, especially in the stomach and
intestine (Fang et al., 1996).
Method: Nothern blot hybridization
Reference: Fang et al., 1996
Method: Whole mount in situ hybridization
Reference: Fang et al., 1996
||Several cells at the center of the vegetal plate
||Prospective SMCs at the tip of archenteron (presumably pigment-forming cells)
||SMCs (presumably pigment-forming cells), endoderm cells near the blastopore, few PMCs, some SMCs
not embedded in ectodermal wall, but scattered in blastocoel, ECs
||Gut (stomach, intestine), ECs
- CyI Strongylocentrotus purpuratus
- CyIIa Strongylocentrotus purpuratus
- CyIIb Strongylocentrotus purpuratus
- CyIIIa Strongylocentrotus purpuratus
- CyIIIb Strongylocentrotus purpuratus
- M actin Strongylocentrotus purpuratus
- LpC2 Lytechinus pictus
- LpC3 Lytechinus pictus
- LpC4 Lytechinus pictus
- LpM Lytechinus pictus
- Sf15a S. franciscanus
- Sf15b S. franciscanus
- PoCy P. ochraceus
- PoM P. ochraceus
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