Paracentrotus lividus Genes in Development: DNA MTase
DNA (cytosine-5)-methyltransferase (DNA MTase)
DNA MTases are the enzymes involved in the modification of the C-5 position of cytosines in the DNA. It is a particularly interesting group of proteins for the implications of DNA methylations in the control of gene expression in eukaryotes (Aniello et al., 1996).
DNA MTase open reading frame potentially encodes a protein of 1612 aa
residues with the molecular mass of 181 kDa.
The putative enzyme sequence shows about 110 additional
aa residues at the N-terminus not found in the enzymes
of other organisms (Aniello et al., 1996).
Nothern blot analysis revealed a single 7.5-kb RNA band.
It is present at low level in the unfertilized eggs
and at a much higher concentration immediately after
fertilization with peak value at the 4-blastomere stage.
The concentration decreases at later stages
until morula and is nearly zero at the blastula
The transcript is again present at a low concentration
at the young gastrula stage and the amount is
nearly constant at all later stages (Aniello et al., 1996).
Method: Nothern blot analysis
Reference: Aniello et al., 1996
- DNA MTase H. sapiens
- DNA MTase Mouse
- DNA MTase Chicken
Comments are welcome to Sveta Surkova
Copyright © 1997 GeNet Team