Gene Networks Database
Strongylocentrotus purpuratus Genes in
Development: Transcription Factors
SpRunt1 is a member of the runt domain family of
transcription factors and a positive regulator of the
aboral ectoderm-specific CyIIIa gene.
Blots of genomic DNA indicate that SpRunt1 is represented as a single gene per gaploid genome
(Coffman J.A. et al.,1996).
SpRunt1 was initially identifired as the 21-kDa polypeptide
(Coffman J.A. et al.,1992), however, conceptual translation
of the SpRunt1 cDNA predicted an approximately 60-kDa product,
which was verified by in vitro translation (Coffman J.A. et. al.,1996).
Since SpRunt1 appears to be encoded in a single
embryonic message, it is likely
that the 21-kDa protein represents a proteolytic
fragment of the full-length translation product.
DNA-binding activity of the 60-kDa protein resides in the
N-terminal half, which includes the runt domain. (Coffman J.A. et. al.,1996).
No maternal SpRunt1 transcripts could be detected.
Transcripts are present in the early embryo
at a constant, low level (approximately 700 per embryo)
throughout early and late cleavage stages.
After the late blastula stage (24 hr of development)
SpRunt1 transcripts rapidly accumulate to an
approximately 10-fold higher level.
Thus the SpRunt1 gene is zygotically activated during
cleavage, and the later sharp increase in the level of SpRunt1 mRNA depends on further
transcriptional activation or/and mRNA
stabilization (Coffman J.A. et. al.,1996).
Temporal accumulation (transcripts/embryo)
Method: probe excess titration
Reference: Coffman J.A. et al., 1996
- runt Drosophila melanogaster
- PEBP2aA Mouse
- PEBP2aB Mouse
- AMLI Homo sapiens
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