Gene Networks Database


Strongylocentrotus purpuratus Genes in Development: Cell-type specific genes


Spec2c


Function

Spec2c is the aboral ectoderm specific gene.
It codes for a calcium-binding protein from the troponin C superfamily (Xiang et al., 1988).
The function of this protein is unknown, its accumulation in the aboral ectoderm cells marks a commitment to a pathway of histospecific differentiation in the embryo (Gan et al., 1993).

Protein

Spec2c is a calcium-binding proteins with the molecular mass of 14,000-17,000 Da (Xiang et al., 1988).
SWISS_PROT: P04111

Subcellular location


Expression Pattern

mRNA level

Temporal accumulation*

Method: RNA blot hybridization
Reference: Hardin et al., 1988

Stage
Egg
Cleavage
Hatching blastula
Midgastrula
Pluteus
Level
-
-
-
+
+
* The probe was specific for Spec2c, Spec2a and other Spec2 related transcripts. Significant accumulation of Spec2c, Spec2a and related Spec2 transcripts is not seen until the gastrula stage and by pluteus stage Spec2a/Spec2c messages decreased to about 65% of their peak abundance.

Spatial localization*

Method: in situ hybridization
Reference: Hardin et al., 1988

Stage
18 hr hatching blastula
22 hr mesenchyme blastula
44 hr late gastrula
60 hr prism/pluteus
75 hr pluteus
Tissue
-
AE
AE
AE
AE
*Spec2c probe hybridizes to both the Spec2c/Spec2a and Spec2d subfamilies, however authors believe that in situ hybridizations with this probe monitor primary the much more abundant Spec2a/Spec2c transcripts.


Sequences

GenBank:

Regulatory Regions


Regulatory Connections

Upstream Genes

Spec2c

Downstream Genes


Evolutionary Homologues


Links


Bibliography


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