Gene Networks Database

LOCUS       PR_SPCYI     1164 bp    DNA             INV       27-MAY-1998
DEFINITION  Strongylocentrotus purpuratus cytoplasmic actin I SpCyI gene,
            promoter region.
DBSOURCE    GENBANK:  name SPU16263, accession U16263
KEYWORDS    CyI
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 1164, DNA binding proteins)
  AUTHORS   Ganster,R., Paul,H. and Katula,K.S.
  TITLE     Analysis of the DNA binding proteins interacting with specific
            upstream sequences of the S. purpuratus CyI actin gene
  JOURNAL   Mol. Reprod. Dev. 33 (4), 392-406 (1992)
  MEDLINE   93112404
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Kissinger,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-1994) Jessica C. Kissinger, Department of
            Biology, Indiana University, Jordan Hall, Bloomington, IN 47405,
            USA
COMMENTS    The CyI actin gene is regulated temporally and spatially within the 
            cells of the early embryo. Using DNase I footprinting, 10 protected 
            regions were identified containing both conserved and apparently 
            novel protein binding sites. Gel mobility shift competition assays 
            confirmed the presence of multiple protein factors which 
            specifically recognize CyI actin upstream sequences. Heterologous 
            gel shift competition assays, combined with other data, indicate 
            both unique and redundant protein-DNA interactions occur in the 
            region. The gel mobility shift analysis detected an increase in 
            binding activity for most of the factors, indicating that early 
            transcriptional activity of CyI actin may involve a general 
            increase in the amount or activity of specific transcription 
            factors [1].
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone="plasmid pCIpN5 derived from the Scheller et al.
                     1981 lambda-28 genomic clone."
     promoter        1..1027
                     /gene="SpCyI"
     TATA_signal     995..999
                     /gene="SpCyI"
     mRNA            1029..
     protein_bind    123..162
                     /evidence=DNase I footprinting
                     /site="RH-L"                      
                     /citation=[1]
                     /note="octamer element"
     protein_bind    219..250
                     /evidence=DNase I footprinting
                     /site="E8-K"                    
                     /citation=[1]
                     /note="octamer element"
     protein_bind    291..309
                     /site="E8-J"                    
                     /citation=[1]
     protein_bind    451..490
                     /evidence=DNase I footprinting
                     /site="C9-HI"                     
                     /citation=[1]
                     /note="homology to sites BC and G"
     protein_bind    553..582
                     /evidence=DNase I footprinting 
                     /site="C9-G"                     
                     /citation=[1]
                     /note="homology to sites BC and HI"
                     /note="octamer-like element"
     protein_bind    631..660
                     /evidence=DNase I footprinting
                     /site="BB-F"                     
                     /citation=[1]
     protein_bind    737..760
                     /evidence=DNase I footprinting
                     /site="BB-E"                     
                     /citation=[1]
     protein_bind    896..927
                     /evidence=DNase I footprinting
                     /site="TB-D"                   
                     /citation=[1]
                     /note="GC-rich"
     protein_bind    935..973
                     /evidence=DNase I footprinting
                     /site="TB-BC"                     
                     /citation=[1]
                     /note="SRE"
                     /note="CCAAT box homology"
                     /note="homology to sites HI and G"
     protein_bind    983..1005
                     /evidence=DNase I footprinting
                     /site="TB-A"                     
                     /citation=[1]
                     /note="TATA box"
ORIGIN      
        1 ataatnatag acgagggagt tatactacgc tgataataaa gcctttatta tgaccaaact
       61 tttattcaag taaaaaataa tcnggttcna ncatttcaag ttactgttat aggatgttca
      121 tcatgatcta tcacyattta gcataatgta tatancttga ggtataaaca ttatgttaac
      181 tttatttttt attattttta ttgtacattt atataatcat tcctgttttt atgcaaatat
      241 tatgtaaaca tcgtatgatt ggtccctgga ctactgcatt gtacattgtg ttggtattaa
      301 taaaccgaaa ttgaattgaa ttgaaatgtg cacattttat gctgtgtatc attcagcttg
      361 aaaaaaattg tgtttgttta ttatgataga tatgcctata atcctgaata ccttagatca
      421 tcttgctatt gctctacatc tgnaggtata gcagaatttc tgaaaaatac atctacattt
      481 tggctatttt cctataactt ctctcgaagg atttcgcata tataggaaac acatagcatg
      541 agatttcgtt ttgcaattcc tttcttacat tttagcatag aatttgaaat caaatgggta
      601 ctgcagcaaa agcatcacat catccatgtt acatttatac tagttatatt ccatgcgaaa
      661 cccatggaga gaagttaaag gcaaaatagc caaaaaaggg ataaaaagag aagaaatggg
      721 gtttgtaaag tgcttcgact atattgtaat aattcgtaaa cactctgcat aatggaactg
      781 agctcatatc atcatggaga gagcatgcct cacgtgtatt tgtgaccttt aaatcagcca
      841 cttgtgcgaa tcagaacctg cgtaggagta aaacagggtg gttttctgag gcgatccccg
      901 ataaggatat taatgaatgc gccgaggcat acgatgcgtc taaatttctc cttatatggt
      961 caaatatgtt tcacgtacaa cgtccggtca ttattataat acctgcttgc tcctgctcct
     1021 cagcctcatt ccaacctcaa ccaagcaaac ttgcaggtgt gatcattctc tctctctttc
     1081 ggtaagtctt taagaatttc ctacgattct cctacgattt ccttagctcs tctacaggat
     1141 catagctcca tagcagcgat tcgcatg