Gene Networks Database

LOCUS       Pr_SPHE        398 bp    DNA             INV       27-APR-1998
DEFINITION  Strongylocentrotus purpuratus SpHE promoter
DBSOURCE    GENBANK:  name S79598, accession S79598 
KEYWORDS    .
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Wei,Z., Angerer,L.M., Gagnon,M.L. and Angerer,R.C.
  TITLE     Characterization of the SpHE promoter that is spatially regulated
            along the animal-vegetal axis of the sea urchin embryo
  JOURNAL   Dev. Biol. 171 (1), 195-211 (1995)
  MEDLINE   96018753
  REMARK    GeNet staff at the IHPC&DB created this entry from the
            original journal article. This sequence comes from Fig. 8.
REFERENCE   2  (bases 1 to 398)
  AUTHORS   Wei,Z., Angerer,L.M., and Angerer,R.C.
  TITLE     Multiple positive cis elements regulate the assymetric expression
            of the SpHE gene along the sea urchin embryo animal-vegetal axis
  JOURNAL   Dev. Biol. 187 (1), 71-78 (1997)
  MEDLINE   97367908
REFERENCE   3  (bases 1 to 398)
  AUTHORS   Wei,Z., Angerer,R.C., and Angerer,L.M.
  TITLE     Identification of a new sea urchin Ets protein, spEts4, by yeast             one-hybrid screening with the hatching enzyme promoter
  JOURNAL   Mol. Cell. Biol. 19 (2), 1271-1278 (1999)
  MEDLINE   
COMMENTS    SpHE promoter is sufficient for high-level, spatially correct
            regulation in early embryo. However neither this nor a longer
            upstream region (-1255 to +96) is sufficienet to shut down the
            transcription from the endogenous SpHE gene shortly after very 
            blastula stage (1). The nonvegetal transcription of SpHE is 
            controlled predominantly, and probably exclusively, by positive
            elements. Positively acting elements appear to be redundunt with
            respect to not only promoter strength but also spatial activity
            of transgenes (1,2).              
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     promoter        1..398
                     /partial
                     /note="hatching enzyme"
                     /gene="SpHE"
     protein_bind    31..41
                     /citation=[1]                     
                     /site="IA"
                     /bound_moiety="I/IV/VII factor"
                     /evidence=EMSA and DNase I footprinting
     protein_bind    45..50
                     /citation=[1]                     
                     /site="IB"
                     /bound_moiety="SpOTx"
                     /evidence=EMSA and DNase I footprinting
     protein_bind    53..70
                     /citation=[3]                    
                     /site="IC"
                     /bound_moiety="SpEts4"
                     /evidence=EMSA and DNase I footprinting   
     protein_bind    71..77
                     /citation=[1]                    
                     /site="IC"
                     /bound_moiety="ets-related factor"
                     /evidence=EMSA and DNase I footprinting
     protein_bind    70..88
                     /citation=[1]
                     /site="II"
                     /evidence=DNase I footprinting
     protein_bind    106..140;
                     /citation=[1]
                     /site="III"
                     /evidence=EMSA
     protein_bind    153..157
                     /citation=[1]
                     /site="IV"
                     /bound_moiety="I/IV/VII factor"
                     /evidence=EMSA and DNase I footprinting
     protein_bind    159..195
                     /citation=[1]
                     /site="V"
                     /bound_moiety=&CCAAT binding protein"
                     /evidence=DNase I footprinting
     protein_bind    194..211
                     /citation=[3]                    
                     /site="IC"
                     /bound_moiety="SpEts4"
                     /evidence=EMSA and DNase I footprinting        
     protein_bind    199..234
                     /citation=[1]
                     /site="VI"
                     /bound_moiety="CCAAT binding protein"
                     /evidence=DNase I footprinting
     protein_bind    296..306
                     /citation=[1]
                     /site="VII"
                     /bound_moiety="I/IV/VII factor"
                     /evidence=EMSA and DNase I footprinting
     mRNA            311..>398
                     /gene="SpHE"

BASE COUNT      130 a     85 c     73 g    110 t
ORIGIN      
        1 gatccgtcct aaaaaatcca tacatttcaa tttctttgtc agggtaatcc aattcaaacc
       61 cggaactacg tagctaggca acaagaaaca tcatccgcag ttccgggaga agattgacat
      121 cctatagcga cgttggtcaa tagtttacta ttgtccgtac aatagtcgtc caatcaaaaa
      181 ggagcttacc aggtacgcgt acggaacagg ccaatcagag cagcgtttgt tagggcgcgg
      241 ttaactagaa acccactttt actgggaatc aaaatcgaat aaaaagccag tgaattgttt
      301 gtgaaaatca ttatcacttc ttatcatcaa gctgtaaaca agctcataca agttagaaag
      361 ttgttttcat ttcaacttaa atcatcgttc gctcttta