LOCUS SUSENDO16C 4692 bp mRNA INV 19-JUL-1994
DEFINITION Strongylocentrotus purpuratus calcium-binding protein (endo16)
mRNA, complete cds.
ACCESSION L34680
NID g511893
KEYWORDS calcium-binding protein.
SOURCE Strongylocentrotus purpuratus (library: lambda gt11) gastrula stage
cDNA to mRNA.
ORGANISM Strongylocentrotus purpuratus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Strongylocentrotidae; Strongylocentrotus.
REFERENCE 1 (bases 1 to 4692)
AUTHORS Soltysik-Espanola,M., Klinzing,D.C., Pfarr,K., Burke,R.D. and
Ernst,S.G.
TITLE Endo16, a large multi-domain protein found on the surface and ECM
of endodermal cells during sea urchin gastrulation, binds calcium
JOURNAL Dev. Biol. (1994) In press
FEATURES Location/Qualifiers
source 1..4692
/organism="Strongylocentrotus purpuratus"
/db_xref="taxon:7668"
/cell_type="endoderm/mesoderm"
/dev_stage="gastrula stage"
/tissue_lib="lambda gt11"
gene 7..4689
/gene="endo16"
CDS 7..4689
/gene="endo16"
/codon_start=1
/product="calcium-binding protein"
/db_xref="PID:g511894"
/translation="MMRLNILLFAVLAVARSMPTELQCDIDEAHTNVGTPRVVDDDSN
SLMISNFELSLDEVDEGFLPEIEYAVSLKSNSDLFQEFLVTAHNIDPHCDHGTMIDKY
DIGTPDEDCPHVIRQAIVSDMRRISFSWKAPACGCVSFRATVKTAEHVYSESSSEKGS
LFKEVCLIGMNKSDEEEEMEAVEEEEVLEEVPFYDEELYGNIERLCEEGNFRGTILPA
VIKECCLLPVQQRRMCLVRVIPGEEDDIHRLDTFCSKIAGNFFNGHPVLADAEGVRRC
CELTSDSDRMVCLSEEKRMHYDEICSLSGASVNNTMEDRLPGRNCCSLQGDLRYNCME
KKALRENQKKRLEERRNMRRKVEKVKRLCNAVDQTPEGDRDSEDYQTCCMVEDVDDAL
ACFREVERDNFDGLCKKQAFYSNGKKERVHPCCRLESEERYECFLVKRSNNQQSRINS
TRAPVPNTQGSTRPTFMQRLCAKLRKDDADDTLIACCQQSSFLKKKRCMDKRQNYALA
KICNASNVSVSYAIVEGESFNAAKKNLHPEHECCILNEEERMSCIRTMRISMSKLRRQ
DSSEKPVRVEAAREQKEQRWVKEEGRQNKAICAAVREDVDMIDEQVVRCCENDGESRR
ECLAVLRRNHFTAICNTDDLFQIDIFSTILRTEDPINDEHRCCMRPNADIRAACFSRI
DRQVAMLSYSPPVIEEVQDIRTELLAVAEFNRIDRVCQESKSATSVELKGRWPVKKCC
RFQNNRRYTCLMDAATKLADQSCDGPPPRVYFPMGFYGEYIPSDYTGPFLDPSHPCCS
LTSDMRQDCIQEASMAVLALRLPMIQVKPLVEFFKELPSDTRFDANHQEDICQAAVDF
QSLYADLENGLDVELNQPSRIQTELQELQDLLAEELTEEEEEQLEQLAEQNEEQLELL
AEQEEEQAELLAEQEEERLEILEEQAQQLAEQNQQNEEEEEEEEEEEEEEEDNRRRKR
RKATRFRRPTANKVRAAAGRSIYEVDSLVHCCQENFVAERNSCMTNFWVQLLDEACAA
LPYLPDSSSATSTGLEYASELAQADNNVLAPAPLTFGGRGDIRTSKAVKSISESTVVS
NYDEDNFRSCCALFGEAQLKCFQMHYSPNAPSSQMESEGSANPSTIGSVSEGYVSESQ
GQESERSESEGSEGEDSEETEGAEEPHEQMESEGGDQEESEGAEQPQEQMESEGQEPE
SEEDVDSGEITDGSDMNMPGPGSSDGGVPLGLTEVDEQTSDGEEPEEPEEPGGEQGPH
EQMESEGEGNESEEEEVEEPQEVIESEGQQSESEDPEFEGEEPEESEGEQEPQEQMES
IVQEPESGADVDSGEVTDGSDMNMPGQGSSDGAGGVPLGLVEVKTSNQEGSEVQEESQ
DPQEQMEGEGQGSESEGEEPEEPEGEQGPQEQVESEGEENESEEEEVEEPQGVMESEG
QQSESEEPEFEGEEPEESEGEQEPQDQMESEGQEPESEEDVDSGEVTDGSDMNMPGQG
SSDGAGGVPLGLVEVKTSNQEGSEVQEESQDPQEQTEGEGQGSESEEEEPEEPEEPEK
EKKKDHSG"
BASE COUNT 1510 a 951 c 1320 g 911 t
ORIGIN
1 gagacgatga tgaggttaaa tattttgctg ttcgcggttt tggccgtggc gcggtcaatg
61 cccacagagt tgcaatgtga catcgacgag gcccatacga atgttggaac accacgagta
121 gtcgacgatg actctaactc actcatgatc tccaactttg agctttcact cgacgaggtc
181 gacgagggct tcctacccga gatcgaatat gcagtcagtc tcaaaagcaa cagcgacctc
241 tttcaagagt tcctggtcac tgcacacaac atcgatcctc attgcgatca cggaacgatg
301 attgataagt acgatatcgg aacgccagat gaagactgcc ctcatgtgat cagacaggca
361 atagtttcgg acatgcgcag aatatcgttc agctggaagg cacccgcatg tggttgcgtt
421 tcttttagag ctactgtcaa gactgctgaa cacgtgtact ctgagtcttc atcggagaag
481 ggctccctct tcaaagaagt gtgtttaatt ggcatgaaca agtcagatga agaagaggag
541 atggaagcgg tggaagagga agaggttttg gaagaagtgc cgttctatga cgaggagctc
601 tatgggaaca ttgaacgact atgcgaggaa ggaaacttta ggggaaccat tctacctgca
661 gtcatcaagg aatgttgtct gcttccagtt caacaacgca gaatgtgtct ggtgcgagtc
721 atcccaggtg aagaagatga tatccacagg ttggatacct tctgctccaa gattgcagga
781 aacttcttca acggtcaccc cgtcttagcc gacgcagagg gcgtgcgacg atgctgtgag
841 ctgactagcg attcggatcg tatggtttgc ctgagcgagg agaaaagaat gcactatgac
901 gaaatctgca gtttgagtgg ggctagcgtg aacaacacca tggaagatcg tttgcccggt
961 cgcaactgtt gcagcctcca gggtgatctc aggtacaact gcatggaaaa gaaggcactg
1021 agggagaatc aaaagaaacg tctggaggag aggcgcaaca tgaggcgcaa ggtggaaaag
1081 gtgaagcgtc tttgcaacgc tgttgaccaa acaccagaag gtgataggga cagtgaggat
1141 taccaaacgt gctgcatggt tgaggacgtg gacgatgcat tggcgtgttt cagggaagtc
1201 gagagggaca actttgatgg attgtgcaag aagcaagcct tctacagcaa cggtaaaaag
1261 gaaagagttc atccgtgctg cagattggag agtgaagaaa ggtatgaatg tttcttggta
1321 aaacgatcga acaaccaaca atcaagaatt aactcaacaa gagcaccagt accgaatact
1381 caaggttcga ctcgaccgac attcatgcag aggttatgtg caaagcttcg aaaggacgat
1441 gcggatgaca cactcatcgc ttgttgtcag caatcatcat ttctcaaaaa gaagagatgc
1501 atggacaaga gacaaaatta tgctttggcc aaaatttgca acgccagcaa cgtaagcgtt
1561 tcatacgcca ttgttgaggg agagagtttc aacgctgcca agaagaacct tcatcccgag
1621 cacgaatgct gtattctgaa cgaagaggag cggatgtcct gcatcagaac aatgaggatt
1681 tcaatgtcca agcttagacg tcaggattca tctgagaagc cggtgcgtgt agaagctgca
1741 cgtgagcaaa aggaacagcg atgggtgaag gaggagggcc gtcagaataa agccatttgc
1801 gctgctgtga gagaagacgt tgatatgata gacgagcagg tcgtccgttg ctgcgaaaac
1861 gacggtgaaa gtcggcggga atgtctagct gttctccgta ggaaccactt taccgctata
1921 tgcaataccg atgacttgtt ccaaatagac attttcagta ccattttgcg cacagaagac
1981 ccaatcaatg acgaacatag gtgttgcatg agaccgaacg ccgatataag agctgcgtgc
2041 ttctctcgca tagatcgcca ggtcgcgatg ctgtcctaca gtcccccagt tattgaagag
2101 gtgcaagata ttcgaacaga gctcctcgcc gttgcagagt tcaacagaat cgacagagtc
2161 tgccaagaat cgaaaagtgc gacctccgtt gaactaaagg gacgatggcc ggtgaaaaag
2221 tgttgtaggt tccagaacaa tcgaaggtat acttgcctga tggacgccgc aacaaaacta
2281 gctgaccaat cgtgcgacgg acctcctcct agagtatact ttccaatggg attctacgga
2341 gaatacatcc caagtgacta cactggccct ttcctcgatc cttcccaccc atgctgttca
2401 ttgaccagtg acatgcgaca agactgcatc caggaggcca gcatggctgt tcttgcattg
2461 cgactaccca tgattcaggt caagccactg gttgagtttt ttaaagagtt gccatctgat
2521 acgcgttttg atgccaatca ccaggaagac atttgccagg ctgcagtaga tttccaaagc
2581 ttatacgctg atcttgaaaa cggcctcgac gtagaactga accagccttc aagaatccag
2641 actgaattac aggagcttca ggaccttcta gcagaagaac tgacagaaga ggaagaggaa
2701 caattggaac aacttgccga gcagaatgaa gaacagttag aattgttagc tgagcaagaa
2761 gaggaacagg cagaattgct ggctgagcaa gaagaggagc gtcttgaaat acttgaggaa
2821 caggctcaac agctagcaga gcagaatcaa cagaatgaag aagaggagga agaggaggag
2881 gaagaggagg aagaggagga agacaatcgc cgacggaaac gaagaaaagc tactaggttc
2941 cggagaccta ctgctaacaa ggtgcgtgcg gctgcgggcc gcagtatcta tgaggtcgac
3001 agtttagttc attgttgcca ggaaaatttt gtggcggaaa gaaattcttg catgacaaac
3061 ttttgggtgc aactgttaga tgaagcatgt gcagcccttc cttacttacc tgattcttct
3121 tctgctacgt ctactggcct ggaatatgct tcagaacttg cacaagccga taataatgtg
3181 ctcgccccag ctccattgac gtttggagga cgcggagaca ttcgaacctc taaagcagta
3241 aagtcaatct cagaatctac agtcgtttcg aattatgatg aagacaactt ccgatcatgc
3301 tgtgctctct ttggggaagc ccaactgaaa tgcttccaaa tgcactatag tcctaatgct
3361 cctagctcac aaatggaaag tgagggatct gcgaatccgt caacgatagg cagtgtgtcc
3421 gagggatatg tatctgaaag ccaggggcaa gaaagtgaga gatctgaaag tgaaggttct
3481 gaaggggagg attccgaaga gacagagggc gctgaagaac cacatgagca aatggaaagt
3541 gaagggggag atcaggaaga gtcagagggc gctgaacaac cacaggagca aatggaaagt
3601 gaaggacaag aacccgagtc agaggaagat gtcgattctg gagaaataac tgatggatcg
3661 gacatgaaca tgccaggacc gggatcatct gacggaggtg ttccactagg actaacggaa
3721 gtcgatgaac aaacatccga tggagaggag cccgaggaac cagaagagcc aggaggagaa
3781 caaggaccac acgaacagat ggagagcgag ggagaaggaa atgaatctga agaagaggag
3841 gttgaggaac cccaggaagt gatagaaagt gaaggacaac agtctgaatc agaagaccca
3901 gaatttgaag gagaagaacc ggaagaatca gaaggagaac aagaaccgca agaacagatg
3961 gaaagtatag tacaagaacc cgagtcgggg gcagatgtcg attctggaga agtaactgac
4021 ggatcggaca tgaacatgcc aggacaggga tcgtctgacg gagcaggagg tgttccacta
4081 ggactagtgg aagtcaaaac atccaaccaa gaggggtctg aagtacaaga agaatcgcag
4141 gacccacagg aacaaatgga aggtgaaggt caaggaagtg aatcggaggg agaagaacca
4201 gaagaaccag aaggagaaca aggaccacaa gaacaggtgg agagcgaagg agaagaaaat
4261 gaatctgaag aagaggaggt ggaagaaccc cagggagtga tggaaagtga aggacaacag
4321 tctgaatcag aagaaccaga atttgaagga gaagaaccgg aagaatcaga aggagaacaa
4381 gaaccacagg accagatgga aagtgaagga caagaacccg agtcagagga agatgtcgat
4441 tctggagaag taactgacgg atcggacatg aacatgccag gacagggatc gtctgacgga
4501 gcaggaggtg ttccactagg actagtggaa gtcaaaacat ccaaccaaga ggggtctgaa
4561 gtacaagaag aatcgcagga cccacaggaa caaacggaag gtgaaggtca aggaagtgaa
4621 tcggaggaag aagaaccaga agaaccagaa gaaccagaaa aagaaaaaaa aaaagatcac
4681 tcaggctgat ga
//