LOCUS       SUSHISES3    2424 bp    DNA             INV       18-MAR-1994
DEFINITION  Sea urchin (S.purpuratus) early histone genes H2B ( H2B alpha ) 
            and H3.
ACCESSION   J01173 J01205 M10375 V01356
NID         g161503
KEYWORDS    histone H2B; histone H3.
            3 of 4
SOURCE      Sea urchin (S.purpuratus) DNA, clones pSp[2,17]; clone pSp2b-1 [4].
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1868 to 1953)
  AUTHORS   Sures,I., Maxam,A., Cohn,R.H. and Kedes,L.H.
  TITLE     Identification and location of the histone H2A and H3 genes by
            sequence analysis of sea urchin (S.purpuratus) DNA cloned in E.
            coli
  JOURNAL   Cell 9, 495-502 (1976)
  MEDLINE   77089442
REFERENCE   2  (bases 392 to 2424)
  AUTHORS   Sures,I., Lowry,J. and Kedes,L.H.
  TITLE     The DNA sequence of sea urchin (s. purpuratus) H2A, H2B and H3
            histone coding and spacer regions
  JOURNAL   Cell 15, 1033-1044 (1978)
  MEDLINE   79084134
REFERENCE   3  (bases 515 to 648)
  AUTHORS   Levy,S., Sures,I. and Kedes,L.H.
  TITLE     Sequence of the 5'-end of Strongylocentrotus purpuratus H2b histone
            mRNA and its location within histone DNA
  JOURNAL   Nature 279, 737-739 (1979)
  MEDLINE   79199774
REFERENCE   4  (bases 1 to 636)
  AUTHORS   Mauron,A., Levy,S., Childs,G. and Kedes,L.
  TITLE     Monocistronic transcription is the physiological mechanism of sea
            urchin embryonic histone gene expression
  JOURNAL   Mol. Cell. Biol. 1 (7), 661-671 (1981)
  MEDLINE   97425329
COMMENT     [3]  mRNA start site.
            All four segments of this entry are from a pair of clones pSp2 and
            pSp17 which cover the early histone gene repeat. This unit is about
            6.5 kb long, contains the H1, H4, H2b, H3 and H2A genes in that
            order, and is repeated several hundred times. The five coding
            regions are separated by DNA spacers not represented in polysomal
            mRNA; there are no reported introns.
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     mRNA            536..>1008
                     /note="H2b mRNA [3]"
     CDS             634..1008
                     /codon_start=1
                     /product="histone H2B"
                     /db_xref="PID:g161508"
                     /translation="MAPTAQVAKKGSKKAVKGTKTAXGGKKRNRKRKESYGIYIYKVL
                     KQVHPDTGISSRAMVIMNSXVXDIFERIAGESSRLAQYNKKXTXSSREIQTAVRLILP
                     GELAKHAVSEGTKAVTKYTTSK"
     CDS             1787..2197
                     /codon_start=1
                     /product="histone H3"
                     /db_xref="PID:g161509"
                     /translation="MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRP
                     GTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTELRFQSSAVMALQEASEAYLV
                     GXXXDTNLCAIHAKRVTIMPKDIQLARRIRGERA"
BASE COUNT      747 a    578 c    477 g    575 t     47 others
ORIGIN      About 300 bp after segment 2.
        1 gcgcctttag atatattata taggttccct ttttcatttg ctgtaaccaa ataattttga
       61 aatacaatgt caaagatatt tcattgctca tttctgttat atcattacgt ttattattat
      121 tgtctatcat tgtgtatatt gtgtgagaag aaatgaaaaa taaattcgct ctttacatat
      181 agagagagct actactacta ctatataatt atatatctcc gagataatgt caaaagtagg
      241 agtgannnnn nnnnnnnnnn tgcaagtatg tcttggataa aaagtctcga catgttccat
      301 attcccatca aaattcatcg tcttcttcaa ctttttcaca tttctcattc tttggggatt
      361 gaattgaaca atgcagacag annnnnnnnn ntgcccgtat gatcactatg tcgccatctc
      421 taggcagggg atggaacagg cactaanctg cgacgcctaa gaccaatgaa aggatcgaga
      481 ccgaggctca tttgcatacg gaccgcagca tacggatccg gccccgtgta taaaaaggaa
      541 aggttctcgc tggccattca cagtatccaa agaatatttg cttgacatac tcgtttcgct
      601 gcatctttac agaccagaaa acctcaattc atcatggctc caacagctca agttgctaag
      661 aaaggctcca agaaggcagt caaaggcacc aagacggccn gcggtggcaa gaagaggaac
      721 aggaaaagga aggagagtta tggaatctac atctacaaag tcctcaagca ggttcatcca
      781 gataccggca tctccagtcg ggccatggtc atcatgaaca gctnngtnna cgacatcttc
      841 gagcgaattg ccggcgaatc ttcccgcctc gctcagtaca acaaaaagtn nacnntcagc
      901 agtcgcgaga ttcagaccgc cgtccgcctc attctccccg gagagctggc aaagcacgct
      961 gtgagcgagg gtaccaaggc agtgacgaaa tacactacct ccaagtagac aggtcatatc
     1021 ctgctctaat tggacataat acaacggccn nacaaataat caagaaagaa tgatatccgt
     1081 agtaatgtag cgtagttgtt tattaaataa ataatgttgt ataacgaata ttaagatata
     1141 atcaagatta cacaatagta tgtaataata ttagccctat ataaatctca ctctcataaa
     1201 tctagactag agcatgaagt gtagtcatga tggtcggaag gaagacgaaa atagttgatt
     1261 aattaaataa aaagaaatac aattcgagat ggcaacatgt cctaaatgat atacgtccat
     1321 gcatcctttc cttccttgaa ngtnnacaga tgcaggttaa gtcttttctc atgattcatc
     1381 accacccagc tccctgcctg cctccctctc atccgccctc ttaagatcag ccgagcagac
     1441 tctcggtcaa acaccttaat catcatttgt tagaatgcat attttcgtaa ttatttggca
     1501 ccactgagtg cgtgagccga attcccggct tgccatgtaa cccatgttgc aaacggtggt
     1561 agcgccacct ttcgacacat tttattgact ttgcacatac ggcatcgcca agcccccttc
     1621 ccgtcacgcg ctaaacaaaa gagcaacccg gttgaccaat caagagagct ttacaaacgg
     1681 caccaggatc ccgcagcaca tataaatagc tgaaaattgc cagtggttct cattcatccc
     1741 gtcactcgta tttgaagtac tgaattacct actgtcccaa gcaactatgg cacgcaccaa
     1801 gcagaccgct cgcaaatcta caggagggaa ggctccccgc aagcagctgg caaccaaagc
     1861 tgccagaaag agtgcccccg ccactggagg agtcaagaag cctcatcgat acaggcctgg
     1921 cacagtcgcc ctgagagaga ttcgccgcta ccagaagagc actgagcttc tcatccgaaa
     1981 actgccattc cagcgtctag tgcgtgagat tgcacaggac ttcaagacag agctacgttt
     2041 ccagagttcc gctgtgatgg cccttcaaga agccagcgag gcatacctag ttggccnnnn
     2101 nngagacacc aacctgtgtg ccatccacgc caagagggtt accatcatgc ccaaagacat
     2161 ccagctcgcc cgtcgaatcc gcggagaacg cgcctagaac catcggtaca gcatgtagcc
     2221 catgcaccgc atacacaaac ggctcttttc agagccacca caaccccaag aaagantcat
     2281 tgatttctaa aatgaacacg atgcatgaca atcgacgcta ttataagaag aaagaaaaaa
     2341 acaacaacaa acatttgtag atcataattg agatgaacga tatgatgaaa tgtagaacat
     2401 aatttagata cacgatatga taaa
//