LOCUS       SPSPE2D3      146 bp    DNA             INV       19-JUN-1992
DEFINITION  Strongylocentrotus purpuratus Spec2d exon 3.
NID         g10308
KEYWORDS    calcium binding protein; Spec2 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 146)
  AUTHORS   Hardin,P.E., Angerer,L.M., Hardin,S.H., Angerer,R.C. and Klein,W.H.
  TITLE     Spec2 genes of Strongylocentrotus purpuratus. Structure and
            differential expression in embryonic aboral ectoderm cells
  JOURNAL   J. Mol. Biol. 202 (3), 417-431 (1988)
  MEDLINE   89011969
COMMENT     see x12773, x14539 - x14543 for Spec2D gene exon seq. Data kindly
            reviewed (05-May-1989) by Klein W.H.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Strongylocentrotus purpuratus"
                     /clone_lib="lambda EMBL3."
     intron          <1..5
                     /note="Intron II (approx. 1.0 kb)"
     precursor_RNA   <1..>146
                     /note="primary transcript"
     CDS             6..140
                     /note="Spec2d protein (AA 14 - 58) (6 is 2nd base in
                     codon) (140 is 1st base in codon)"
     mRNA            6..140
                     /note="Exon 3"
     intron          141..>146
                     /note="Intron III (approx. 1.1 kb)"
BASE COUNT       43 a     27 c     34 g     42 t
        1 tctagaatac aagacaaaat tcgatgcctt tgacaggaat aacgatggca acttccctac
       61 tatgttcttg ggaaatgcga tgaaatcagt gggtcatgtc cttactgcgg ctgagcttga
      121 aaattctaga cgtgttcgta gtaagt