LOCUS       SPSPE2D1      536 bp    DNA             INV       19-JUN-1992
DEFINITION  Strongylocentrotus purpuratus Spec2d gene 5'-flank and exon 1.
ACCESSION   X12773
NID         g10305
KEYWORDS    calcium binding protein; Spec2 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Hardin,P.E., Angerer,L.M., Hardin,S.H., Angerer,R.C. and Klein,W.H.
  TITLE     Spec2 genes of Strongylocentrotus purpuratus. Structure and
            differential expression in embryonic aboral ectoderm cells
  JOURNAL   J. Mol. Biol. 202 (3), 417-431 (1988)
  MEDLINE   89011969
COMMENT     see x12773, x14539 - x14543 for SpecD gene exon seq. Data kindly
            reviewed (05-May-1989) by Klein W.H.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone_lib="lambda EMBL3."
     promoter        385..391
                     /note="TATA-box"
     precursor_RNA   415..>536
                     /note="primary transcript"
     mRNA            415..530
                     /note="Exon 1"
     CDS             528..530
                     /note="Spec2d protein (AA 1 - 1)"
                     /codon_start=1
                     /db_xref="PID:g578510"
                     /db_xref="SWISS-PROT:P15844"
                     /translation="M"
     intron          531..>536
                     /note="Intron I (approx. 4.9 kb)"
BASE COUNT      180 a    107 c     87 g    161 t      1 others
ORIGIN
        1 catcgaggta acgcctccaa cattacaaag tacgtcaaac cttgtttcta aaaatcctag
       61 aaagatgttc taagtatcat gattacgggg cgaactattt ttgcacttcc cacaaacttt
      121 tggttatcaa ctttctcctt gtcactggta ctggtcctgg gcaaattgta ttattangac
      181 ggatatcacg attatgctat tactaaacta aatagcaaaa taatgaaaga gtaatatagg
      241 aaacatcttg gcaaatacta tagaccttga ttcgttcatt ggcgcaaatg atcactacgg
      301 actataaaac tggtcttaaa actaaagtaa caacaacaac aacaacaaca ataccagcat
      361 atggtcacgt gatttatatg tccgtataat actctttgac atcactgcac ccttgtcact
      421 ttttgaaagc aagtcaacca taagagaggt ataggtaggc tacctgagat ccagagaaat
      481 tttgcttttt atcttcaatt ttgccggatc tatcccaatt tctaaagatg gtaagt
//