Gene Networks Database
Strongylocentrotus purpuratus Genes in Development:
Cell type specific genes; Actins
Cytoskeletal actin IIIb
The CyIIIb actin gene codes for a cytoskeletal type
actin (Flutzanis et al., 1989). Actin represents one of the most abundunt and best characterized
structural components in eucaryotic cells, involved in a wide variety
of cytoskeletal, locomotive, and tension-generating functions. Five functional cytoskeletal
actin genes are organized in two linkage groups within the genome:
CyI-CyIIa-CyIIb and CyIIIa-CyIIIb.
CyIIIb protein belongs to a family of the cytoskeletal
actins (Flutzanis et al., 1989).
CyIIIb sequence differs
significantly (approx.50%) from the sequences of other sea urchin
cytoskeletal actin proteins within an amino acid region
from position 257 to 267.
Developmental studies of actin mRNA prevalence have been caried out using
several sensitive methods including in situ hybridization (Cox et al., 1986), molecular
titration with single-strand RNA probes (Lee et al., 1986), RNA gel blot hybridization, and
cDNA analysis (Shott et al., 1984; Crain et al., 1981; Garcia et al., 1984).
All of the S. purpuratus cytoskeletal actin genes are
activated at 8-9 hr of development, a time when 64-128
cell embryos are predominant in large cultures.
CyIIIb message accumulation is transcriptionally regulated (Lee et al., 1992).
CyIIIb mRNA accumulation is first observed at 10 hr postfertilization.
Considerable amounts of mRNA do not accumulate until well
into the pluteus stage 3 days later.
The CyIIIb actin gene is exclusively expressed in the
aboral ectoderm by a combinatorial repression in all
other cell lineages of the developing embryo (Flytzanis et al., 1989).
Method: in situ hybridization
Reference: Cox et al., 1986
Upstream regulatory region
- CyI S.purpuratus
- CyIIa S.purpuratus
- CyIIb S.purpuratus
- CyIIIa S.purpuratus
- M actin S.purpuratus
- other actins
UrchiNetSearch the GeNet
Comments are welcome to Kate G. Savostyanova
Copyright © 1997 GeNet Team